Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.

The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernize...

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Autores: Muñoz Santiago, Silvia, Méndez, Lucía, Dasilva, Gabriel, Torres, Josep Lluís, Ramos-Romero, Sara, Romeu, Marta, Nogués, M. R., Medina, Isabel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/171465
Acesso em linha:http://hdl.handle.net/10261/171465
Access Level:Acceso aberto
Palavra-chave:Oxidative stress
High-fat high-sucrose diet
Liver protein damage
Marine omega-3 fatty acids
Carbonylation
Fish oils
Sprague-dawley rat
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spelling Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.Muñoz Santiago, SilviaMéndez, LucíaDasilva, GabrielTorres, Josep LluísRamos-Romero, SaraRomeu, MartaNogués, M. R.Medina, IsabelOxidative stressHigh-fat high-sucrose dietLiver protein damageMarine omega-3 fatty acidsCarbonylationFish oilsSprague-dawley ratThe present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver.This research was founded by the Spanish Ministry of Science and Innovation (grant number AGL2013-49079-C2-1-R) and The Consejo Superior de Investigaciones Científicas (CSIC). Both are gratefully acknowledged for the doctoral fellowship to Silvia Muñoz Xunta de Galicia is also thankfully recognized for the financial support of the postdoctoral contract to Lucía Méndez The authors thank Lorena Barros, Salomé Lois and Maria Jesus González for their technical assistance.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Xunta de GaliciaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2018201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/171465reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-49079-C2-1-Rhttps://doi.org/10.3390/md16100353Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1714652026-05-22T06:33:51Z
dc.title.none.fl_str_mv Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
title Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
spellingShingle Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
Muñoz Santiago, Silvia
Oxidative stress
High-fat high-sucrose diet
Liver protein damage
Marine omega-3 fatty acids
Carbonylation
Fish oils
Sprague-dawley rat
title_short Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
title_full Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
title_fullStr Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
title_full_unstemmed Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
title_sort Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils.
dc.creator.none.fl_str_mv Muñoz Santiago, Silvia
Méndez, Lucía
Dasilva, Gabriel
Torres, Josep Lluís
Ramos-Romero, Sara
Romeu, Marta
Nogués, M. R.
Medina, Isabel
author Muñoz Santiago, Silvia
author_facet Muñoz Santiago, Silvia
Méndez, Lucía
Dasilva, Gabriel
Torres, Josep Lluís
Ramos-Romero, Sara
Romeu, Marta
Nogués, M. R.
Medina, Isabel
author_role author
author2 Méndez, Lucía
Dasilva, Gabriel
Torres, Josep Lluís
Ramos-Romero, Sara
Romeu, Marta
Nogués, M. R.
Medina, Isabel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Xunta de Galicia
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Oxidative stress
High-fat high-sucrose diet
Liver protein damage
Marine omega-3 fatty acids
Carbonylation
Fish oils
Sprague-dawley rat
topic Oxidative stress
High-fat high-sucrose diet
Liver protein damage
Marine omega-3 fatty acids
Carbonylation
Fish oils
Sprague-dawley rat
description The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/171465
url http://hdl.handle.net/10261/171465
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-49079-C2-1-R
https://doi.org/10.3390/md16100353
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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