A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus

Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due...

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Detalles Bibliográficos
Autores: Blasco, Lucía, Veiga Crespo, Patricia, Viñas, Miquel, Villa, Tomás G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/52756
Acceso en línea:https://hdl.handle.net/2445/52756
Access Level:acceso abierto
Palabra clave:Fermentació
Saccharomyces cerevisiae
Genòmica
Vi
Fermentation
Genomics
Wine
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spelling A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianusBlasco, LucíaVeiga Crespo, PatriciaViñas, MiquelVilla, Tomás G.FermentacióSaccharomyces cerevisiaeGenòmicaViFermentationSaccharomyces cerevisiaeGenomicsWineYeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.Spanish Society for Microbiology (SEM) and Viguera Editores SL2014201420112014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion6 p.application/pdfhttps://hdl.handle.net/2445/52756Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.149International Microbiology, 2011, vol. 14, p. 201-206http://dx.doi.org/10.2436/20.1501.01.149cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011http://creativecommons.org/licenses/by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/527562026-05-29T05:05:01Z
dc.title.none.fl_str_mv A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
title A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
spellingShingle A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
Blasco, Lucía
Fermentació
Saccharomyces cerevisiae
Genòmica
Vi
Fermentation
Saccharomyces cerevisiae
Genomics
Wine
title_short A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
title_full A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
title_fullStr A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
title_full_unstemmed A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
title_sort A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
dc.creator.none.fl_str_mv Blasco, Lucía
Veiga Crespo, Patricia
Viñas, Miquel
Villa, Tomás G.
author Blasco, Lucía
author_facet Blasco, Lucía
Veiga Crespo, Patricia
Viñas, Miquel
Villa, Tomás G.
author_role author
author2 Veiga Crespo, Patricia
Viñas, Miquel
Villa, Tomás G.
author2_role author
author
author
dc.subject.none.fl_str_mv Fermentació
Saccharomyces cerevisiae
Genòmica
Vi
Fermentation
Saccharomyces cerevisiae
Genomics
Wine
topic Fermentació
Saccharomyces cerevisiae
Genòmica
Vi
Fermentation
Saccharomyces cerevisiae
Genomics
Wine
description Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.
publishDate 2011
dc.date.none.fl_str_mv 2011
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/52756
url https://hdl.handle.net/2445/52756
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.149
International Microbiology, 2011, vol. 14, p. 201-206
http://dx.doi.org/10.2436/20.1501.01.149
dc.rights.none.fl_str_mv cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
http://creativecommons.org/licenses/by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
http://creativecommons.org/licenses/by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6 p.
application/pdf
dc.publisher.none.fl_str_mv Spanish Society for Microbiology (SEM) and Viguera Editores SL
publisher.none.fl_str_mv Spanish Society for Microbiology (SEM) and Viguera Editores SL
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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