Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes

Our objective is to evaluate the regional regenerative potential of calvarial bone in critical- sized defects in a rabbit model using novel nanostructured silica-loaded membranes doped with zinc or doxycycline. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5 wt% of SiO2 n...

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Autores: Toledano, Manuel, Vallecillo, Cristina, Gutiérrez Corrales, Aída, Torres-Lagares, Daniel, Toledano-Osorio, Manuel, Serrera Figallo, María de los Ángeles
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/154746
Acceso en línea:https://hdl.handle.net/11441/154746
https://doi.org/10.3390/polym14102078
Access Level:acceso abierto
Palabra clave:Zinc
Doxycycline
Silica
Guided bone regeneration
Bone
Osteoid
Polymer
Membrane
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spelling Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped MembranesToledano, ManuelVallecillo, CristinaGutiérrez Corrales, AídaTorres-Lagares, DanielToledano-Osorio, ManuelSerrera Figallo, María de los ÁngelesZincDoxycyclineSilicaGuided bone regenerationBoneOsteoidPolymerMembraneOur objective is to evaluate the regional regenerative potential of calvarial bone in critical- sized defects in a rabbit model using novel nanostructured silica-loaded membranes doped with zinc or doxycycline. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5 wt% of SiO2 nanoparticles (HOOC-Si-Membranes) were doped with zinc (Zn-HOOC-Si- Membrane) or doxycycline (Dox-HOOC-Si-Membrane). Critical bone defects were created on six New-Zealand-breed rabbit skulls and covered with the membranes. A sham defect without a membrane was used as the control. After six weeks, a histological analysis (toluidine blue technique) was employed to determine the area percentages of newly formed bone, osteoid bone, and soft tissue. The measurements were performed by dividing the total defect area into top (close to the membrane) and bottom (close to the dura mater) regions, or peripheral (adjacent to the old bone) and central (the sum of the remaining zones) regions. The peripheral regions of the defects showed higher osteogenic capacity than the central areas when the membranes were present. The proportion of new bone adjacent to the dura was similar to that adjacent to the membrane only when the HOOC-Si-Membranes and Zn-HOOC-Si-Membranes were used, indicating a direct osteoinductive effect of the membranes.MDPIEstomatología2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/154746https://doi.org/10.3390/polym14102078reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)Ingléspoymers, 14 (10), 2078.https://www.mdpi.com/2073-4360/14/10/2078info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1547462026-06-17T12:51:07Z
dc.title.none.fl_str_mv Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
title Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
spellingShingle Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
Toledano, Manuel
Zinc
Doxycycline
Silica
Guided bone regeneration
Bone
Osteoid
Polymer
Membrane
title_short Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
title_full Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
title_fullStr Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
title_full_unstemmed Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
title_sort Histomorphometric Analysis of Differential Regional Bone Regeneration Induced by Distinct Doped Membranes
dc.creator.none.fl_str_mv Toledano, Manuel
Vallecillo, Cristina
Gutiérrez Corrales, Aída
Torres-Lagares, Daniel
Toledano-Osorio, Manuel
Serrera Figallo, María de los Ángeles
author Toledano, Manuel
author_facet Toledano, Manuel
Vallecillo, Cristina
Gutiérrez Corrales, Aída
Torres-Lagares, Daniel
Toledano-Osorio, Manuel
Serrera Figallo, María de los Ángeles
author_role author
author2 Vallecillo, Cristina
Gutiérrez Corrales, Aída
Torres-Lagares, Daniel
Toledano-Osorio, Manuel
Serrera Figallo, María de los Ángeles
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Estomatología
dc.subject.none.fl_str_mv Zinc
Doxycycline
Silica
Guided bone regeneration
Bone
Osteoid
Polymer
Membrane
topic Zinc
Doxycycline
Silica
Guided bone regeneration
Bone
Osteoid
Polymer
Membrane
description Our objective is to evaluate the regional regenerative potential of calvarial bone in critical- sized defects in a rabbit model using novel nanostructured silica-loaded membranes doped with zinc or doxycycline. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5 wt% of SiO2 nanoparticles (HOOC-Si-Membranes) were doped with zinc (Zn-HOOC-Si- Membrane) or doxycycline (Dox-HOOC-Si-Membrane). Critical bone defects were created on six New-Zealand-breed rabbit skulls and covered with the membranes. A sham defect without a membrane was used as the control. After six weeks, a histological analysis (toluidine blue technique) was employed to determine the area percentages of newly formed bone, osteoid bone, and soft tissue. The measurements were performed by dividing the total defect area into top (close to the membrane) and bottom (close to the dura mater) regions, or peripheral (adjacent to the old bone) and central (the sum of the remaining zones) regions. The peripheral regions of the defects showed higher osteogenic capacity than the central areas when the membranes were present. The proportion of new bone adjacent to the dura was similar to that adjacent to the membrane only when the HOOC-Si-Membranes and Zn-HOOC-Si-Membranes were used, indicating a direct osteoinductive effect of the membranes.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/154746
https://doi.org/10.3390/polym14102078
url https://hdl.handle.net/11441/154746
https://doi.org/10.3390/polym14102078
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv poymers, 14 (10), 2078.
https://www.mdpi.com/2073-4360/14/10/2078
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
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