The Reverse Transcriptases Associated with CRISPR-Cas Systems

CRISPR (clustered regularly interspaced short palindromic repeats) and associated proteins (Cas) act as adaptive immune systems in bacteria and archaea. Some CRISPR-Cas systems have been found to be associated with putative reverse transcriptases (RT), and an RT-Cas1 fusion associated with a type II...

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Detalhes bibliográficos
Autores: Toro, Nicolás, Martínez-Abarca, Francisco, González-Delgado, Alejandro
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/167437
Acesso em linha:http://hdl.handle.net/10261/167437
Access Level:Acceso aberto
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spelling The Reverse Transcriptases Associated with CRISPR-Cas SystemsToro, NicolásMartínez-Abarca, FranciscoGonzález-Delgado, AlejandroCRISPR (clustered regularly interspaced short palindromic repeats) and associated proteins (Cas) act as adaptive immune systems in bacteria and archaea. Some CRISPR-Cas systems have been found to be associated with putative reverse transcriptases (RT), and an RT-Cas1 fusion associated with a type III-B system has been shown to acquire RNA spacers in vivo. Nevertheless, the origin and evolutionary relationships of these RTs and associated CRISPR-Cas systems remain largely unknown. We performed a comprehensive phylogenetic analysis of these RTs and associated Cas1 proteins, and classified their CRISPR-Cas modules. These systems were found predominantly in bacteria, and their presence in archaea may be due to a horizontal gene transfer event. These RTs cluster into 12 major clades essentially restricted to particular phyla, suggesting host-dependent functioning. The RTs and associated Cas1 proteins may have largely coevolved. They are, therefore, subject to the same selection pressures, which may have led to coadaptation within particular protein complexes. Furthermore, our results indicate that the association of an RT with a CRISPR-Cas system has occurred on multiple occasions during evolution.This work was supported by research grant BIO2014-51953-P from the Spanish Ministerio de Ciencia e Innovación, now known as the Ministerio de Economía y Competitividad, including ERDF (European Regional Development Funds). A.G.-D. was supported by a FPU predoctoral fellowship from the Ministerio de Economía y Competitividad.Peer ReviewedNature Publishing GroupEuropean CommissionMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2018201820172018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/167437reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#MINECO/BIO2014-51953-PSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1674372026-05-22T06:33:51Z
dc.title.none.fl_str_mv The Reverse Transcriptases Associated with CRISPR-Cas Systems
title The Reverse Transcriptases Associated with CRISPR-Cas Systems
spellingShingle The Reverse Transcriptases Associated with CRISPR-Cas Systems
Toro, Nicolás
title_short The Reverse Transcriptases Associated with CRISPR-Cas Systems
title_full The Reverse Transcriptases Associated with CRISPR-Cas Systems
title_fullStr The Reverse Transcriptases Associated with CRISPR-Cas Systems
title_full_unstemmed The Reverse Transcriptases Associated with CRISPR-Cas Systems
title_sort The Reverse Transcriptases Associated with CRISPR-Cas Systems
dc.creator.none.fl_str_mv Toro, Nicolás
Martínez-Abarca, Francisco
González-Delgado, Alejandro
author Toro, Nicolás
author_facet Toro, Nicolás
Martínez-Abarca, Francisco
González-Delgado, Alejandro
author_role author
author2 Martínez-Abarca, Francisco
González-Delgado, Alejandro
author2_role author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description CRISPR (clustered regularly interspaced short palindromic repeats) and associated proteins (Cas) act as adaptive immune systems in bacteria and archaea. Some CRISPR-Cas systems have been found to be associated with putative reverse transcriptases (RT), and an RT-Cas1 fusion associated with a type III-B system has been shown to acquire RNA spacers in vivo. Nevertheless, the origin and evolutionary relationships of these RTs and associated CRISPR-Cas systems remain largely unknown. We performed a comprehensive phylogenetic analysis of these RTs and associated Cas1 proteins, and classified their CRISPR-Cas modules. These systems were found predominantly in bacteria, and their presence in archaea may be due to a horizontal gene transfer event. These RTs cluster into 12 major clades essentially restricted to particular phyla, suggesting host-dependent functioning. The RTs and associated Cas1 proteins may have largely coevolved. They are, therefore, subject to the same selection pressures, which may have led to coadaptation within particular protein complexes. Furthermore, our results indicate that the association of an RT with a CRISPR-Cas system has occurred on multiple occasions during evolution.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
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info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/167437
url http://hdl.handle.net/10261/167437
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
MINECO/BIO2014-51953-P
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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