Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease

Glial vulnerability to prions is assessed in murine Creutzfeldt-Jakob disease (CJD) using the tg340 mouse line expressing four-fold human PrP M129 levels on a mouse PrP null background at different days following intracerebral inoculation of sCJD MM1 brain tissues homogenates. The mRNA expression of...

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Autores: Andrés Benito, Pol, Carmona Murillo, Margarita, Douet, Jean Yves, Cassard, Hervé, Andreoletti, Olivier, Ferrer, Isidro (Ferrer Abizanda)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/179297
Acceso en línea:https://hdl.handle.net/2445/179297
Access Level:acceso abierto
Palabra clave:Malaltia de Creutzfeldt-Jakob
Malalties per prions
Astròcits
Creutzfeldt-Jakob disease
Prion diseases
Astrocytes
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spelling Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob diseaseAndrés Benito, PolCarmona Murillo, MargaritaDouet, Jean YvesCassard, HervéAndreoletti, OlivierFerrer, Isidro (Ferrer Abizanda)Malaltia de Creutzfeldt-JakobMalalties per prionsAstròcitsCreutzfeldt-Jakob diseasePrion diseasesAstrocytesGlial vulnerability to prions is assessed in murine Creutzfeldt-Jakob disease (CJD) using the tg340 mouse line expressing four-fold human PrP M129 levels on a mouse PrP null background at different days following intracerebral inoculation of sCJD MM1 brain tissues homogenates. The mRNA expression of several astrocyte markers, including glial fibrillary acidic protein (gfap), aquaporin-4 (aqp4), solute carrier family 16, member 4 (mct4), mitochondrial pyruvate carrier 1 (mpc1) and solute carrier family 1, member 2 (glial high-affinity glutamate transporter, slc1a2) increases at 120 and 180 dpi. In contrast, the mRNA expression of oligodendrocyte and myelin markers oligodendrocyte transcription factor 1 (olig1), olig2, neural/glial antigen 2 (cspg), solute carrier family 16, member 1 (mct1), myelin basic protein (mbp), myelin oligodendrocyte glycoprotein (mog) and proteolipid protein 1 (plp1) is preserved. Yet, myelin regulatory factor (myrf) mRNA is increased at 180 dpi. In the striatum, a non-significant increase in the number of GFAP-positive astrocytes and Iba1-immunoreactive microglia occurs at 160 dpi; a significant increase in the number of astrocytes and microglia, and a significant reduction in the number of Olig2-immunoreactive oligodendrocytes occur at 180 dpi. A decrease of MBP, but not PLP1, immunoreactivity is also observed in the striatal fascicles. These observations confirm the vulnerability and the reactive responses of astrocytes, together with the microgliosis at middle stages of prion diseases. More importantly, these findings show oligodendrocyte vulnerability and myelin alterations at advanced stages of murine CJD. They confirm oligodendrocyte involvement in the pathogenesis of CJD.Informa UK Limited2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/179297Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1080/19336896.2021.1935105Prion, 2021, vol. 15, num. 1, p. 112–120https://doi.org/10.1080/19336896.2021.1935105cc by (c) Andrés Benito, Pol et al., 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1792972026-05-27T06:46:51Z
dc.title.none.fl_str_mv Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
title Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
spellingShingle Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
Andrés Benito, Pol
Malaltia de Creutzfeldt-Jakob
Malalties per prions
Astròcits
Creutzfeldt-Jakob disease
Prion diseases
Astrocytes
title_short Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
title_full Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
title_fullStr Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
title_full_unstemmed Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
title_sort Differential astrocyte and oligodendrocyte vulnerability in murine Creutzfeldt-Jakob disease
dc.creator.none.fl_str_mv Andrés Benito, Pol
Carmona Murillo, Margarita
Douet, Jean Yves
Cassard, Hervé
Andreoletti, Olivier
Ferrer, Isidro (Ferrer Abizanda)
author Andrés Benito, Pol
author_facet Andrés Benito, Pol
Carmona Murillo, Margarita
Douet, Jean Yves
Cassard, Hervé
Andreoletti, Olivier
Ferrer, Isidro (Ferrer Abizanda)
author_role author
author2 Carmona Murillo, Margarita
Douet, Jean Yves
Cassard, Hervé
Andreoletti, Olivier
Ferrer, Isidro (Ferrer Abizanda)
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Malaltia de Creutzfeldt-Jakob
Malalties per prions
Astròcits
Creutzfeldt-Jakob disease
Prion diseases
Astrocytes
topic Malaltia de Creutzfeldt-Jakob
Malalties per prions
Astròcits
Creutzfeldt-Jakob disease
Prion diseases
Astrocytes
description Glial vulnerability to prions is assessed in murine Creutzfeldt-Jakob disease (CJD) using the tg340 mouse line expressing four-fold human PrP M129 levels on a mouse PrP null background at different days following intracerebral inoculation of sCJD MM1 brain tissues homogenates. The mRNA expression of several astrocyte markers, including glial fibrillary acidic protein (gfap), aquaporin-4 (aqp4), solute carrier family 16, member 4 (mct4), mitochondrial pyruvate carrier 1 (mpc1) and solute carrier family 1, member 2 (glial high-affinity glutamate transporter, slc1a2) increases at 120 and 180 dpi. In contrast, the mRNA expression of oligodendrocyte and myelin markers oligodendrocyte transcription factor 1 (olig1), olig2, neural/glial antigen 2 (cspg), solute carrier family 16, member 1 (mct1), myelin basic protein (mbp), myelin oligodendrocyte glycoprotein (mog) and proteolipid protein 1 (plp1) is preserved. Yet, myelin regulatory factor (myrf) mRNA is increased at 180 dpi. In the striatum, a non-significant increase in the number of GFAP-positive astrocytes and Iba1-immunoreactive microglia occurs at 160 dpi; a significant increase in the number of astrocytes and microglia, and a significant reduction in the number of Olig2-immunoreactive oligodendrocytes occur at 180 dpi. A decrease of MBP, but not PLP1, immunoreactivity is also observed in the striatal fascicles. These observations confirm the vulnerability and the reactive responses of astrocytes, together with the microgliosis at middle stages of prion diseases. More importantly, these findings show oligodendrocyte vulnerability and myelin alterations at advanced stages of murine CJD. They confirm oligodendrocyte involvement in the pathogenesis of CJD.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/179297
url https://hdl.handle.net/2445/179297
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1080/19336896.2021.1935105
Prion, 2021, vol. 15, num. 1, p. 112–120
https://doi.org/10.1080/19336896.2021.1935105
dc.rights.none.fl_str_mv cc by (c) Andrés Benito, Pol et al., 2021
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Andrés Benito, Pol et al., 2021
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Informa UK Limited
publisher.none.fl_str_mv Informa UK Limited
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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