Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy

Pregnancy is a period in a woman’s life associated with an increased risk of developing lower extremity chronic venous disease (CVD). Pregnancy-associated CVD is associated with changes in placental villi. We investigated angiogenesis and lymphangiogenesis in the placental villi of women with CVD du...

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Autores: Ortega, Miguel Ángel, Saez, M.A., Fraile-Martínez, O., Asúnsolo, Ángel, Pekarek, L., Bravo, C., Coca, S., Sainz, F., Álvarez-Mon, Melchor, García-Honduvilla, N.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/219229
Acceso en línea:http://hdl.handle.net/10261/219229
Access Level:acceso abierto
Palabra clave:Chronic venous disease
pregnancy
CD31
podoplanin (D2-40), Flt-1
PIGF
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spelling Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancyOrtega, Miguel ÁngelSaez, M.A.Fraile-Martínez, O.Asúnsolo, ÁngelPekarek, L.Bravo, C.Coca, S.Sainz, F.Álvarez-Mon, MelchorGarcía-Honduvilla, N.Chronic venous diseasepregnancyCD31podoplanin (D2-40), Flt-1PIGFPregnancy is a period in a woman’s life associated with an increased risk of developing lower extremity chronic venous disease (CVD). Pregnancy-associated CVD is associated with changes in placental villi. We investigated angiogenesis and lymphangiogenesis in the placental villi of women with CVD during pregnancy compared with healthy controls with no history of CVD (HC). An observational, analytical, and prospective cohort study was conducted on 114 women in their third trimester of pregnancy (32 weeks). Sixty-two participants were clinically diagnosed with CVD. In parallel, 52 controls with no history of CVD (HC) were studied. Gene and protein expression of CD31, podoplanin (D2-40), Flt-1, and placental growth factor (PIGF) was analysed by real-time polymerase chain reaction (RT-qPCR) and immunohistochemistry. CD31 and D2-40 gene expression was significantly greater in the placental villi of women with CVD, as were the numbers of vessels positive for CD31 and D2-40. Significantly higher gene and protein expression of Flt-1 and PIGF was observed in the placental villi of women with CVD. Histological analysis showed more placental villi with periodic acid of Schiff (PAS)-positive material in women with CVD. Our results show a connection between pregnancy-associated CVD and leading to higher proangiogenic and lymphangiogenic activity in placental villi.Molecular Diversity Preservation InternationalConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/219229reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Españolhttp://dx.doi.org/10.3390/ijms21072487Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2192292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
title Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
spellingShingle Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
Ortega, Miguel Ángel
Chronic venous disease
pregnancy
CD31
podoplanin (D2-40), Flt-1
PIGF
title_short Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
title_full Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
title_fullStr Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
title_full_unstemmed Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
title_sort Increased angiogenesis and lymphangiogenesis in the placental villi of women with chronic venous disease during pregnancy
dc.creator.none.fl_str_mv Ortega, Miguel Ángel
Saez, M.A.
Fraile-Martínez, O.
Asúnsolo, Ángel
Pekarek, L.
Bravo, C.
Coca, S.
Sainz, F.
Álvarez-Mon, Melchor
García-Honduvilla, N.
author Ortega, Miguel Ángel
author_facet Ortega, Miguel Ángel
Saez, M.A.
Fraile-Martínez, O.
Asúnsolo, Ángel
Pekarek, L.
Bravo, C.
Coca, S.
Sainz, F.
Álvarez-Mon, Melchor
García-Honduvilla, N.
author_role author
author2 Saez, M.A.
Fraile-Martínez, O.
Asúnsolo, Ángel
Pekarek, L.
Bravo, C.
Coca, S.
Sainz, F.
Álvarez-Mon, Melchor
García-Honduvilla, N.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chronic venous disease
pregnancy
CD31
podoplanin (D2-40), Flt-1
PIGF
topic Chronic venous disease
pregnancy
CD31
podoplanin (D2-40), Flt-1
PIGF
description Pregnancy is a period in a woman’s life associated with an increased risk of developing lower extremity chronic venous disease (CVD). Pregnancy-associated CVD is associated with changes in placental villi. We investigated angiogenesis and lymphangiogenesis in the placental villi of women with CVD during pregnancy compared with healthy controls with no history of CVD (HC). An observational, analytical, and prospective cohort study was conducted on 114 women in their third trimester of pregnancy (32 weeks). Sixty-two participants were clinically diagnosed with CVD. In parallel, 52 controls with no history of CVD (HC) were studied. Gene and protein expression of CD31, podoplanin (D2-40), Flt-1, and placental growth factor (PIGF) was analysed by real-time polymerase chain reaction (RT-qPCR) and immunohistochemistry. CD31 and D2-40 gene expression was significantly greater in the placental villi of women with CVD, as were the numbers of vessels positive for CD31 and D2-40. Significantly higher gene and protein expression of Flt-1 and PIGF was observed in the placental villi of women with CVD. Histological analysis showed more placental villi with periodic acid of Schiff (PAS)-positive material in women with CVD. Our results show a connection between pregnancy-associated CVD and leading to higher proangiogenic and lymphangiogenic activity in placental villi.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/219229
url http://hdl.handle.net/10261/219229
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/ijms21072487
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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