PCR: una nueva herramienta para el estudio de hongos ectomicorrícicos
[EN]PCR (Polymerase Chain Reaction) is a simple technique that allows the specific amplification of particular regions of the genome of living organisms to facilitate their study. In the last few years, this and other complementary techniques have opened the field of the molecular mycology, whose ap...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 1997 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/45815 |
| Acceso en línea: | http://hdl.handle.net/10261/45815 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA PCR RFLP técnicas moleculares hongos ectomicorrizas |
| Sumario: | [EN]PCR (Polymerase Chain Reaction) is a simple technique that allows the specific amplification of particular regions of the genome of living organisms to facilitate their study. In the last few years, this and other complementary techniques have opened the field of the molecular mycology, whose applications in detection, identification and classification of fungi are increasing every day. In this article, the first of a series dealing with the Molecular Techniques that can be used in Mycology, we explain the basic principles of both the PCR and two other complementary techniques commonly used for the molecular analysis of the amplified regions: RFLPs (Restriction Fragment Length Polymorphisms)and sequencing (determination of the primary structure of the nucleic acids). |
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