TFIIIC binding to alu elements controls gene expression via chromatin looping and histone acetylation

How repetitive elements, epigenetic modifications, and architectural proteins interact ensuring proper genome expression remains poorly understood. Here, we report regulatory mechanisms unveiling a central role of Alu elements (AEs) and RNA polymerase III transcription factor C (TFIIIC) in structura...

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Detalles Bibliográficos
Autores: Ferrari, Roberto, De Llobet, Lara Isabel, Di Vona, Chiara, 1981-, Le Dily, François, Vidal Ocabo, Enrique, Lloutas, Antonios, Quilez Oliete, Javier, Jochem, Laura, Cuts, Erin, Dieci, Giorgio, Vannini, Alessandro, Teichmann, Martin, de la Luna, Susana, Beato, Miguel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/44261
Acceso en línea:http://hdl.handle.net/10230/44261
http://dx.doi.org/10.1016/j.molcel.2019.10.020
Access Level:acceso abierto
Palabra clave:Expressió gènica
Cromatina
Histones
Descripción
Sumario:How repetitive elements, epigenetic modifications, and architectural proteins interact ensuring proper genome expression remains poorly understood. Here, we report regulatory mechanisms unveiling a central role of Alu elements (AEs) and RNA polymerase III transcription factor C (TFIIIC) in structurally and functionally modulating the genome via chromatin looping and histone acetylation. Upon serum deprivation, a subset of AEs pre-marked by the activity-dependent neuroprotector homeobox Protein (ADNP) and located near cell-cycle genes recruits TFIIIC, which alters their chromatin accessibility by direct acetylation of histone H3 lysine-18 (H3K18). This facilitates the contacts of AEs with distant CTCF sites near promoter of other cell-cycle genes, which also become hyperacetylated at H3K18. These changes ensure basal transcription of cell-cycle genes and are critical for their re-activation upon serum re-exposure. Our study reveals how direct manipulation of the epigenetic state of AEs by a general transcription factor regulates 3D genome folding and expression.

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