Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure

Wastewater-based epidemiology (WBE) relies on the assessment and interpretation of levels of biomarkers in wastewater originating from a well-defined community. It has provided unique information on spatial and temporal trends of licit and illicit drug consumption, and has also the potential to give...

ver descrição completa

Detalhes bibliográficos
Autores: Campos-Mañas, Marina, Fabregat-Safont, David, Hernández, Félix, de Rijke, Eva, de Voogt, Pim, van Wezel, Annemarie, Bijlsma, Lubertus
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/55444
Acesso em linha:http://hdl.handle.net/10230/55444
http://dx.doi.org/10.1016/j.chemosphere.2022.135684
Access Level:Acceso aberto
Palavra-chave:Exposure
Pesticide urinary metabolites
UHPLC-MS/MS
Wastewater surveillance
Wastewater-based epidemiology
id ES_abb7aee14cc77bcc4614abe4561d7e8d
oai_identifier_str oai:recercat.cat:10230/55444
network_acronym_str ES
network_name_str España
repository_id_str
spelling Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposureCampos-Mañas, MarinaFabregat-Safont, DavidHernández, Félixde Rijke, Evade Voogt, Pimvan Wezel, AnnemarieBijlsma, LubertusExposurePesticide urinary metabolitesUHPLC-MS/MSWastewater surveillanceWastewater-based epidemiologyWastewater-based epidemiology (WBE) relies on the assessment and interpretation of levels of biomarkers in wastewater originating from a well-defined community. It has provided unique information on spatial and temporal trends of licit and illicit drug consumption, and has also the potential to give complementary information on human exposure to chemicals. Here, we focus on the accurate quantification of pesticide biomarkers (i.e., predominantly urinary metabolites) in influent wastewater at the ng L-1 level to be used for WBE. In the present study, an advanced analytical methodology has been developed based on ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), for the simultaneous determination of 11 specific human biomarkers of triazines, urea herbicides, pyrethroids and organophosphates in urban wastewater. The sample treatment consisted of solid-phase extraction using Oasis HLB cartridges. Direct injection of the samples was also tested for all compounds, as a simple and rapid way to determine these compounds without sample manipulation (i.e., minimizing potential analytical errors). However, if extraction recoveries are satisfactory, SPE is the preferred approach that allow reaching lower concertation levels. Six isotopically labelled internal standards were evaluated and used to correct for matrix effects. Due to the difficulties associated with this type of analysis, special emphasis has been placed on the analytical challenges encountered. The satisfactory validated methodology was applied to urban wastewater samples collected from different locations across Europe revealing the presence of 2,6-EA, 3,4-DCA, 3-PBA and 4-HSA i.e, metabolites of metolachlor-s, urea herbicides, pyrethroids and chlorpropham, respectively. Preliminary data reported in this paper illustrate the applicability of this analytical approach for assessing human exposure to pesticides through WBE.Elsevier202320232022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/55444http://dx.doi.org/10.1016/j.chemosphere.2022.135684reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglés0045-6535/ © 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/554442026-05-29T05:05:01Z
dc.title.none.fl_str_mv Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
title Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
spellingShingle Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
Campos-Mañas, Marina
Exposure
Pesticide urinary metabolites
UHPLC-MS/MS
Wastewater surveillance
Wastewater-based epidemiology
title_short Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
title_full Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
title_fullStr Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
title_full_unstemmed Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
title_sort Analytical research of pesticide biomarkers in wastewater with application to study spatial differences in human exposure
dc.creator.none.fl_str_mv Campos-Mañas, Marina
Fabregat-Safont, David
Hernández, Félix
de Rijke, Eva
de Voogt, Pim
van Wezel, Annemarie
Bijlsma, Lubertus
author Campos-Mañas, Marina
author_facet Campos-Mañas, Marina
Fabregat-Safont, David
Hernández, Félix
de Rijke, Eva
de Voogt, Pim
van Wezel, Annemarie
Bijlsma, Lubertus
author_role author
author2 Fabregat-Safont, David
Hernández, Félix
de Rijke, Eva
de Voogt, Pim
van Wezel, Annemarie
Bijlsma, Lubertus
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Exposure
Pesticide urinary metabolites
UHPLC-MS/MS
Wastewater surveillance
Wastewater-based epidemiology
topic Exposure
Pesticide urinary metabolites
UHPLC-MS/MS
Wastewater surveillance
Wastewater-based epidemiology
description Wastewater-based epidemiology (WBE) relies on the assessment and interpretation of levels of biomarkers in wastewater originating from a well-defined community. It has provided unique information on spatial and temporal trends of licit and illicit drug consumption, and has also the potential to give complementary information on human exposure to chemicals. Here, we focus on the accurate quantification of pesticide biomarkers (i.e., predominantly urinary metabolites) in influent wastewater at the ng L-1 level to be used for WBE. In the present study, an advanced analytical methodology has been developed based on ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), for the simultaneous determination of 11 specific human biomarkers of triazines, urea herbicides, pyrethroids and organophosphates in urban wastewater. The sample treatment consisted of solid-phase extraction using Oasis HLB cartridges. Direct injection of the samples was also tested for all compounds, as a simple and rapid way to determine these compounds without sample manipulation (i.e., minimizing potential analytical errors). However, if extraction recoveries are satisfactory, SPE is the preferred approach that allow reaching lower concertation levels. Six isotopically labelled internal standards were evaluated and used to correct for matrix effects. Due to the difficulties associated with this type of analysis, special emphasis has been placed on the analytical challenges encountered. The satisfactory validated methodology was applied to urban wastewater samples collected from different locations across Europe revealing the presence of 2,6-EA, 3,4-DCA, 3-PBA and 4-HSA i.e, metabolites of metolachlor-s, urea herbicides, pyrethroids and chlorpropham, respectively. Preliminary data reported in this paper illustrate the applicability of this analytical approach for assessing human exposure to pesticides through WBE.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/55444
http://dx.doi.org/10.1016/j.chemosphere.2022.135684
url http://hdl.handle.net/10230/55444
http://dx.doi.org/10.1016/j.chemosphere.2022.135684
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416293496520704
score 15.812455

Registros relacionados