Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari

A set of 16 microsatellite markers was characterized for Lear’s macaw (Anodorhynchus leari) using DNA samples from cap-tive individuals. Extending this molecular toolkit, including the use of samples from wild individuals, is expected to provide the required power of resolution for pedigree inferenc...

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Autores: Pacífico, Erica C., Sánchez-Montes, Gregorio, Miyaki, Cristina Y., Tella, José Luis
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/239259
Acceso en línea:http://hdl.handle.net/10261/239259
Access Level:acceso abierto
Palabra clave:Microsatellite loci
Parrots
Conservation genetics
Lear’s macaw
Endangered species
http://metadata.un.org/sdg/15
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
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spelling Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leariPacífico, Erica C.Sánchez-Montes, GregorioMiyaki, Cristina Y.Tella, José LuisMicrosatellite lociParrotsConservation geneticsLear’s macawEndangered specieshttp://metadata.un.org/sdg/15Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity lossA set of 16 microsatellite markers was characterized for Lear’s macaw (Anodorhynchus leari) using DNA samples from cap-tive individuals. Extending this molecular toolkit, including the use of samples from wild individuals, is expected to provide the required power of resolution for pedigree inference of both wild and captive individuals, and could support research on the genetic structure of wild populations. We characterize a set of 15 microsatellite markers optimized for the Lear’s macaw, developed from a microsatellite-enriched library in a three-step procedure. Primer pairs were initially designed for 62 micro-satellites with > 7 tandem repetitions. After amplification of DNA of five wild individuals from different localities, 22 loci seemed to be polymorphic and were further tested on 12 wild nestling samples. Fifteen unlinked loci showed unambiguous peaks and low to moderate polymorphism levels. The combination of the four most polymorphic markers allowed individual identification even of putative sibs.These markers complement previously described microsatellites developed for A. leariand constitute a fundamental genetic toolkit for the investigation of the genetics of both wild and captive populations, thus assisting integrated management plans for the conservation of this globally endangered species.Loro Parque Fundación (101-2015) funded this genetic labo-ratory work. ECP was supported by an international PhD fellowship granted by CAPES (the Brazilian Federal Agency for the Support and Evaluation of Graduate Education). CYM was granted by CNPq (303713/2015-1, 306204/2019-3).Peer reviewedSpringer NatureLoro Parque FundaciónCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/239259reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://link.springer.com/article/10.1007/s11033-020-05812-wSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2392592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
title Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
spellingShingle Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
Pacífico, Erica C.
Microsatellite loci
Parrots
Conservation genetics
Lear’s macaw
Endangered species
http://metadata.un.org/sdg/15
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
title_short Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
title_full Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
title_fullStr Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
title_full_unstemmed Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
title_sort Isolation and characterization of 15 new microsatellite markers for the globally endangered Lear’s macaw Anodorhynchus leari
dc.creator.none.fl_str_mv Pacífico, Erica C.
Sánchez-Montes, Gregorio
Miyaki, Cristina Y.
Tella, José Luis
author Pacífico, Erica C.
author_facet Pacífico, Erica C.
Sánchez-Montes, Gregorio
Miyaki, Cristina Y.
Tella, José Luis
author_role author
author2 Sánchez-Montes, Gregorio
Miyaki, Cristina Y.
Tella, José Luis
author2_role author
author
author
dc.contributor.none.fl_str_mv Loro Parque Fundación
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Microsatellite loci
Parrots
Conservation genetics
Lear’s macaw
Endangered species
http://metadata.un.org/sdg/15
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
topic Microsatellite loci
Parrots
Conservation genetics
Lear’s macaw
Endangered species
http://metadata.un.org/sdg/15
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
description A set of 16 microsatellite markers was characterized for Lear’s macaw (Anodorhynchus leari) using DNA samples from cap-tive individuals. Extending this molecular toolkit, including the use of samples from wild individuals, is expected to provide the required power of resolution for pedigree inference of both wild and captive individuals, and could support research on the genetic structure of wild populations. We characterize a set of 15 microsatellite markers optimized for the Lear’s macaw, developed from a microsatellite-enriched library in a three-step procedure. Primer pairs were initially designed for 62 micro-satellites with > 7 tandem repetitions. After amplification of DNA of five wild individuals from different localities, 22 loci seemed to be polymorphic and were further tested on 12 wild nestling samples. Fifteen unlinked loci showed unambiguous peaks and low to moderate polymorphism levels. The combination of the four most polymorphic markers allowed individual identification even of putative sibs.These markers complement previously described microsatellites developed for A. leariand constitute a fundamental genetic toolkit for the investigation of the genetics of both wild and captive populations, thus assisting integrated management plans for the conservation of this globally endangered species.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/239259
url http://hdl.handle.net/10261/239259
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://link.springer.com/article/10.1007/s11033-020-05812-w
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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