Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study

Purpose: To develop a predictable and reproducible model of knee osteoarthritis after medial meniscus posterior root release. Methods: Posteromedial meniscal root tears were created in 12 White New Zealand rabbit knees. The contralateral limbs were used as healthy controls. The animals were euthaniz...

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Detalhes bibliográficos
Autores: Dzidzishvili, Lika, Lopez Torres, Irene Isabel, Guerrero, Carlos Carnero, Calvo Crespo, Emilio
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/714218
Acesso em linha:http://hdl.handle.net/10486/714218
https://dx.doi.org/10.1186/s40634-022-00501-y
Access Level:acceso abierto
Palavra-chave:animal model
early osteoarthritis
knee osteoarthritis
meniscal root tear
rabbit model
Medicina
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spelling Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo studyDzidzishvili, LikaLopez Torres, Irene IsabelGuerrero, Carlos CarneroCalvo Crespo, Emilioanimal modelearly osteoarthritisknee osteoarthritismeniscal root tearrabbit modelMedicinaPurpose: To develop a predictable and reproducible model of knee osteoarthritis after medial meniscus posterior root release. Methods: Posteromedial meniscal root tears were created in 12 White New Zealand rabbit knees. The contralateral limbs were used as healthy controls. The animals were euthanized at 16 weeks postoperatively; tissue samples of femoral and tibial articular cartilage were collected and processed for macro and microscopic analyses to detect signs of early degeneration. Clinical evaluation of the weight-bearing status on the affected knee was conducted at 0-, 4-, 8-, and 16-weeks postoperatively. Results: Early and severe osteoarthritic changes were the hallmark and the main findings after 16-weeks post-surgery. Macroscopically, extensive osteoarthritic changes were observed across the femoral condyle and tibial plateau. Microscopic finding included ulcerations, fissures, fibrillations, pitting, and loss of the superficial layer. Cellularity was diminished, the normal pattern of distribution in columns was lost, and subchondral bone exposure was also evident. Conclusions: This study describes a novel model of knee osteoarthritis that may guide the development of tailored interventions to delay or prevent knee osteoarthritis. This knowledge could shift the current treatment paradigm toward more conservative and knee salvageable treatment options and increase surgeons’ awareness of this injury pattern. Such considerations may have a positive impact on clinical decision-making and subsequent patient-reported clinical outcomes. Design: Controlled laboratory study. Level of evidence: IIWiley Open AccessDepartamento de MedicinaFacultad de Medicina20222022-12-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/714218https://dx.doi.org/10.1186/s40634-022-00501-yreponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7142182026-06-23T12:46:27Z
dc.title.none.fl_str_mv Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
title Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
spellingShingle Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
Dzidzishvili, Lika
animal model
early osteoarthritis
knee osteoarthritis
meniscal root tear
rabbit model
Medicina
title_short Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
title_full Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
title_fullStr Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
title_full_unstemmed Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
title_sort Developing an experimental model of early knee osteoarthritis after medial meniscus posterior root release: an in vivo study
dc.creator.none.fl_str_mv Dzidzishvili, Lika
Lopez Torres, Irene Isabel
Guerrero, Carlos Carnero
Calvo Crespo, Emilio
author Dzidzishvili, Lika
author_facet Dzidzishvili, Lika
Lopez Torres, Irene Isabel
Guerrero, Carlos Carnero
Calvo Crespo, Emilio
author_role author
author2 Lopez Torres, Irene Isabel
Guerrero, Carlos Carnero
Calvo Crespo, Emilio
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Medicina
Facultad de Medicina
dc.subject.none.fl_str_mv animal model
early osteoarthritis
knee osteoarthritis
meniscal root tear
rabbit model
Medicina
topic animal model
early osteoarthritis
knee osteoarthritis
meniscal root tear
rabbit model
Medicina
description Purpose: To develop a predictable and reproducible model of knee osteoarthritis after medial meniscus posterior root release. Methods: Posteromedial meniscal root tears were created in 12 White New Zealand rabbit knees. The contralateral limbs were used as healthy controls. The animals were euthanized at 16 weeks postoperatively; tissue samples of femoral and tibial articular cartilage were collected and processed for macro and microscopic analyses to detect signs of early degeneration. Clinical evaluation of the weight-bearing status on the affected knee was conducted at 0-, 4-, 8-, and 16-weeks postoperatively. Results: Early and severe osteoarthritic changes were the hallmark and the main findings after 16-weeks post-surgery. Macroscopically, extensive osteoarthritic changes were observed across the femoral condyle and tibial plateau. Microscopic finding included ulcerations, fissures, fibrillations, pitting, and loss of the superficial layer. Cellularity was diminished, the normal pattern of distribution in columns was lost, and subchondral bone exposure was also evident. Conclusions: This study describes a novel model of knee osteoarthritis that may guide the development of tailored interventions to delay or prevent knee osteoarthritis. This knowledge could shift the current treatment paradigm toward more conservative and knee salvageable treatment options and increase surgeons’ awareness of this injury pattern. Such considerations may have a positive impact on clinical decision-making and subsequent patient-reported clinical outcomes. Design: Controlled laboratory study. Level of evidence: II
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-12-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/714218
https://dx.doi.org/10.1186/s40634-022-00501-y
url http://hdl.handle.net/10486/714218
https://dx.doi.org/10.1186/s40634-022-00501-y
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley Open Access
publisher.none.fl_str_mv Wiley Open Access
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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