NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex
15 p.-6 fig.-2 tab.
| Autores: | , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/390044 |
| Acceso en línea: | http://hdl.handle.net/10261/390044 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacterial cell division FtsZ MinC NMR Protein complex prediction |
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NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complexMachado, Luciana E. S. F.Castellen, PatriciaBlasios, ValdirRibeiro-Filho, Helder V.Bisson-Filho, Alexandre W.Benites Pariente, Jhonatan S.Nogueira, Maria L. C.Sforça, MaurizioHonorato, Rodrigo V.Lopes-de-Oliveira, Paulo S.Salinas, Roberto K.Andreu, José ManuelZeri, Ana C.Gueiros-Filho, Frederico J.Bacterial cell divisionFtsZMinCNMRProtein complex prediction15 p.-6 fig.-2 tab.The Min system is a key spatial regulator of cell division in rod-shaped bacteria and the first FtsZ-negative modulator to be recognized. Nevertheless, despite extensive genetic and in vitro studies, the molecular mechanism used by MinC to inhibit Z-ring formation remains incompletely understood. The crystallization of FtsZ in complex with other negative regulators such as SulA and MciZ has provided important structural information to corroborate in vitro experiments and establish the mechanism of Z-ring antagonism by these modulators. However, MinC and FtsZ have so far eluded co-crystallization, probably because their complex is too unstable to be crystallized. To gain structural insight into the mechanism of action of MinC, we determined the solution structure of the N-terminal domain of Bacillus subtilis MinC, and through NMR titration experiments and mutagenesis identified the binding interfaces involved in the MinCN-FtsZ interaction. By using our experimental results as restraints in docking, we also constructed a molecular model for the FtsZ:MinCN complex and validated it by molecular dynamics. The model shows that MinCN binding overlaps with the FtsZ polymerization interface on the C-terminal globular subdomain of FtsZ and, thus, provides a structural basis for MinCN inhibition of FtsZ filament formation. Given that the C-terminal polymerization interface of FtsZ corresponds to the plus end of FtsZ filaments, we propose that capping is the main mechanism employed by MinC to antagonize FtsZ polymerization.This work was supported by FAPESP grants 10/51866-0, 16/05203-5, 20/02061-0 (to F.J.G.F) and fellowships 17/17692-3 (to L.E.S.F.M.) and 10/51870-7 (to P.C.), and by the Spanish Ministry of Science (MCIN) grant BFU2014-51823-R (to J.M.A).Peer reviewedElsevierFundação de Amparo à Pesquisa do Estado de São PauloMinisterio de Ciencia e Innovación (España)Castellen, Patricia [0000-0003-4718-740X]Ribeiro-Filho, Helder V. [0000-0001-8471-207X]Bisson-Filho, Alexandre W. [0000-0002-5940-7230]Benites Pariente, Jhonatan S. [0000-0001-6595-0478]Salinas, Roberto K. [0000-0003-1032-7683]Andreu, José Manuel [0000-0001-8064-6933]Gueiros-Filho, Frederico J. [0000-0001-5425-2439]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/390044reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//BFU2014-51823-Rhttps://doi.org/10.1016/j.jbc.2025.108169Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3900442026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| title |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| spellingShingle |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex Machado, Luciana E. S. F. Bacterial cell division FtsZ MinC NMR Protein complex prediction |
| title_short |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| title_full |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| title_fullStr |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| title_full_unstemmed |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| title_sort |
NMR study of the interaction between MinC and FtsZ and modeling of the FtsZ:MinC complex |
| dc.creator.none.fl_str_mv |
Machado, Luciana E. S. F. Castellen, Patricia Blasios, Valdir Ribeiro-Filho, Helder V. Bisson-Filho, Alexandre W. Benites Pariente, Jhonatan S. Nogueira, Maria L. C. Sforça, Maurizio Honorato, Rodrigo V. Lopes-de-Oliveira, Paulo S. Salinas, Roberto K. Andreu, José Manuel Zeri, Ana C. Gueiros-Filho, Frederico J. |
| author |
Machado, Luciana E. S. F. |
| author_facet |
Machado, Luciana E. S. F. Castellen, Patricia Blasios, Valdir Ribeiro-Filho, Helder V. Bisson-Filho, Alexandre W. Benites Pariente, Jhonatan S. Nogueira, Maria L. C. Sforça, Maurizio Honorato, Rodrigo V. Lopes-de-Oliveira, Paulo S. Salinas, Roberto K. Andreu, José Manuel Zeri, Ana C. Gueiros-Filho, Frederico J. |
| author_role |
author |
| author2 |
Castellen, Patricia Blasios, Valdir Ribeiro-Filho, Helder V. Bisson-Filho, Alexandre W. Benites Pariente, Jhonatan S. Nogueira, Maria L. C. Sforça, Maurizio Honorato, Rodrigo V. Lopes-de-Oliveira, Paulo S. Salinas, Roberto K. Andreu, José Manuel Zeri, Ana C. Gueiros-Filho, Frederico J. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Fundação de Amparo à Pesquisa do Estado de São Paulo Ministerio de Ciencia e Innovación (España) Castellen, Patricia [0000-0003-4718-740X] Ribeiro-Filho, Helder V. [0000-0001-8471-207X] Bisson-Filho, Alexandre W. [0000-0002-5940-7230] Benites Pariente, Jhonatan S. [0000-0001-6595-0478] Salinas, Roberto K. [0000-0003-1032-7683] Andreu, José Manuel [0000-0001-8064-6933] Gueiros-Filho, Frederico J. [0000-0001-5425-2439] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Bacterial cell division FtsZ MinC NMR Protein complex prediction |
| topic |
Bacterial cell division FtsZ MinC NMR Protein complex prediction |
| description |
15 p.-6 fig.-2 tab. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/390044 |
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http://hdl.handle.net/10261/390044 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//BFU2014-51823-R https://doi.org/10.1016/j.jbc.2025.108169 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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