Early anther ablation triggers parthenocarpic fruit development in tomato

Fruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male-sterile transgenic plants that produce...

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Autores: Medina, Mónica, Roque Mesa, Edelín Marta, Pineda, Benito, Cañas, Luis, Rodriguez-Concepcion, Manuel, Pío Beltrán, José, Gómez Mena, Concepción
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/248558
Acceso en línea:http://hdl.handle.net/10261/248558
Access Level:acceso abierto
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spelling Early anther ablation triggers parthenocarpic fruit development in tomatoMedina, MónicaRoque Mesa, Edelín MartaPineda, BenitoCañas, LuisRodriguez-Concepcion, ManuelPío Beltrán, JoséGómez Mena, ConcepciónFruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male-sterile transgenic plants that produce parthenocarpic fruits in two tomato cultivars (Micro-Tom and Moneymaker). We generated male-sterility using the cytotoxic gene barnase targeted to the anthers with the PsEND1 anther-specific promoter. The ovaries of these plants grew in the absence of fertilization producing seedless, parthenocarpic fruits. Early anther ablation is essential to trigger the developing of the transgenic ovaries into fruits, in the absence of the signals usually generated during pollination and fertilization. Ovaries are fully functional and can be manually pollinated to obtain seeds. The transgenic plants obtained in the commercial cultivar Moneymaker show that the parthenocarpic development of the fruit does not have negative consequences in fruit quality. Throughout metabolomic analyses of the tomato fruits, we have identified two elite lines which showed increased levels of several health promoting metabolites and volatile compounds. Thus, early anther ablation can be considered a useful tool to promote fruit set and to obtain seedless and good quality fruits in tomato plants. These plants are also useful parental lines to be used in hybrid breeding approaches.This work was supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICINN, AGL2009-07617 to M.M.) and the Ramón y Cajal Program (RYC-2007-00627 to C.G.-M.).Peer reviewedWiley-VCHMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/248558reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1111/pbi.12069Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2485582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Early anther ablation triggers parthenocarpic fruit development in tomato
title Early anther ablation triggers parthenocarpic fruit development in tomato
spellingShingle Early anther ablation triggers parthenocarpic fruit development in tomato
Medina, Mónica
title_short Early anther ablation triggers parthenocarpic fruit development in tomato
title_full Early anther ablation triggers parthenocarpic fruit development in tomato
title_fullStr Early anther ablation triggers parthenocarpic fruit development in tomato
title_full_unstemmed Early anther ablation triggers parthenocarpic fruit development in tomato
title_sort Early anther ablation triggers parthenocarpic fruit development in tomato
dc.creator.none.fl_str_mv Medina, Mónica
Roque Mesa, Edelín Marta
Pineda, Benito
Cañas, Luis
Rodriguez-Concepcion, Manuel
Pío Beltrán, José
Gómez Mena, Concepción
author Medina, Mónica
author_facet Medina, Mónica
Roque Mesa, Edelín Marta
Pineda, Benito
Cañas, Luis
Rodriguez-Concepcion, Manuel
Pío Beltrán, José
Gómez Mena, Concepción
author_role author
author2 Roque Mesa, Edelín Marta
Pineda, Benito
Cañas, Luis
Rodriguez-Concepcion, Manuel
Pío Beltrán, José
Gómez Mena, Concepción
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Fruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male-sterile transgenic plants that produce parthenocarpic fruits in two tomato cultivars (Micro-Tom and Moneymaker). We generated male-sterility using the cytotoxic gene barnase targeted to the anthers with the PsEND1 anther-specific promoter. The ovaries of these plants grew in the absence of fertilization producing seedless, parthenocarpic fruits. Early anther ablation is essential to trigger the developing of the transgenic ovaries into fruits, in the absence of the signals usually generated during pollination and fertilization. Ovaries are fully functional and can be manually pollinated to obtain seeds. The transgenic plants obtained in the commercial cultivar Moneymaker show that the parthenocarpic development of the fruit does not have negative consequences in fruit quality. Throughout metabolomic analyses of the tomato fruits, we have identified two elite lines which showed increased levels of several health promoting metabolites and volatile compounds. Thus, early anther ablation can be considered a useful tool to promote fruit set and to obtain seedless and good quality fruits in tomato plants. These plants are also useful parental lines to be used in hybrid breeding approaches.
publishDate 2013
dc.date.none.fl_str_mv 2013
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/248558
url http://hdl.handle.net/10261/248558
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/pbi.12069
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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