A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp

It is almost 50 years since amoeba-associated granulomatosis was first described in goldfish (Carassius auratus), but the aetiologic agent has never been identified. In this study, a new archamoeba species, Endolimax carassius, is characterised as the causative agent of systemic granulomatous diseas...

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Autores: Constenla Matalobos, Maria|||0000-0002-7672-123X, Palenzuela Ruiz, Oswaldo|||0000-0001-7702-6098
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:273172
Acceso en línea:https://ddd.uab.cat/record/273172
https://dx.doi.org/urn:doi:10.3390/ani13050935
Access Level:acceso abierto
Palabra clave:Parasites
Fish
Archamoebae
Systemic granulomatosis
Carassius auratus
Iodamoeba
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spelling A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax sppConstenla Matalobos, Maria|||0000-0002-7672-123XPalenzuela Ruiz, Oswaldo|||0000-0001-7702-6098ParasitesFishArchamoebaeSystemic granulomatosisCarassius auratusIodamoebaIt is almost 50 years since amoeba-associated granulomatosis was first described in goldfish (Carassius auratus), but the aetiologic agent has never been identified. In this study, a new archamoeba species, Endolimax carassius, is characterised as the causative agent of systemic granulomatous disease in goldfish. Phylogenetic analyses determined the new goldfish parasite species as a genotype sister of Endolimax piscium, the causative agent of systemic granulomatous disease in Senegalese sole. The fish genotypes clustered with other available genotypes from mammals in a well-supported Endolimax clade within the Mastigamoebidae. Endolimax is a genus of intestinal amoebae which stands among the least known human protists. Previous studies on amoebic systemic granulomatosis of a marine fish (Solea senegalensis) resulted in the unexpected characterization of a new organism which was related to Endolimax and named E. piscium. The existence of multiple reports of systemic granulomatosis caused presumptively by unidentified amoebae in goldfish lead us to investigate the organism involved in goldfish disease. Analysed goldfish presented small whitish nodules in the kidney, which correspond to chronic granulomatous inflammatory reactions with a ring-layer of amoebae in the periphery. Amoebae were amitochondriate and were located in a parasitophorous vacuole within macrophages, as previous studies on this condition in goldfish and other freshwater fish pointed out. SSU rDNA characterization confirmed a new Endolimax lineage which appears closely related to E. piscium, but the molecular evidence, distinct pathological features and lack of ecological overlapping between the hosts support their assignment to a new species, E. carassius. The results support the existence of a considerable unexplored diversity of Endolimax spp. among fish, and their proper characterization can contribute to an understanding of Archamoebae evolution and pathogenic potential. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/273172https://dx.doi.org/urn:doi:10.3390/ani13050935reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2731722026-06-06T12:50:31Z
dc.title.none.fl_str_mv A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
title A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
spellingShingle A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
Constenla Matalobos, Maria|||0000-0002-7672-123X
Parasites
Fish
Archamoebae
Systemic granulomatosis
Carassius auratus
Iodamoeba
title_short A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
title_full A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
title_fullStr A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
title_full_unstemmed A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
title_sort A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp
dc.creator.none.fl_str_mv Constenla Matalobos, Maria|||0000-0002-7672-123X
Palenzuela Ruiz, Oswaldo|||0000-0001-7702-6098
author Constenla Matalobos, Maria|||0000-0002-7672-123X
author_facet Constenla Matalobos, Maria|||0000-0002-7672-123X
Palenzuela Ruiz, Oswaldo|||0000-0001-7702-6098
author_role author
author2 Palenzuela Ruiz, Oswaldo|||0000-0001-7702-6098
author2_role author
dc.subject.none.fl_str_mv Parasites
Fish
Archamoebae
Systemic granulomatosis
Carassius auratus
Iodamoeba
topic Parasites
Fish
Archamoebae
Systemic granulomatosis
Carassius auratus
Iodamoeba
description It is almost 50 years since amoeba-associated granulomatosis was first described in goldfish (Carassius auratus), but the aetiologic agent has never been identified. In this study, a new archamoeba species, Endolimax carassius, is characterised as the causative agent of systemic granulomatous disease in goldfish. Phylogenetic analyses determined the new goldfish parasite species as a genotype sister of Endolimax piscium, the causative agent of systemic granulomatous disease in Senegalese sole. The fish genotypes clustered with other available genotypes from mammals in a well-supported Endolimax clade within the Mastigamoebidae. Endolimax is a genus of intestinal amoebae which stands among the least known human protists. Previous studies on amoebic systemic granulomatosis of a marine fish (Solea senegalensis) resulted in the unexpected characterization of a new organism which was related to Endolimax and named E. piscium. The existence of multiple reports of systemic granulomatosis caused presumptively by unidentified amoebae in goldfish lead us to investigate the organism involved in goldfish disease. Analysed goldfish presented small whitish nodules in the kidney, which correspond to chronic granulomatous inflammatory reactions with a ring-layer of amoebae in the periphery. Amoebae were amitochondriate and were located in a parasitophorous vacuole within macrophages, as previous studies on this condition in goldfish and other freshwater fish pointed out. SSU rDNA characterization confirmed a new Endolimax lineage which appears closely related to E. piscium, but the molecular evidence, distinct pathological features and lack of ecological overlapping between the hosts support their assignment to a new species, E. carassius. The results support the existence of a considerable unexplored diversity of Endolimax spp. among fish, and their proper characterization can contribute to an understanding of Archamoebae evolution and pathogenic potential.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/273172
https://dx.doi.org/urn:doi:10.3390/ani13050935
url https://ddd.uab.cat/record/273172
https://dx.doi.org/urn:doi:10.3390/ani13050935
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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