Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging

Adenosine is a neuromodulator that has been involved in aging and neurodegenerative diseases as Alzheimer's disease (AD). In the present work, we analyzed the possible modulation of purine metabolites, 5'nucleotidase (5′NT) and adenosine deaminase (ADA) activities, and adenosine monophosph...

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Autores: Sánchez-Melgar, Alejandro, Albasanz, José Luis, Pallàs i Llibería, Mercè, 1964-, Martín, Mairena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/171435
Acceso en línea:https://hdl.handle.net/2445/171435
Access Level:acceso abierto
Palabra clave:Adenosina
Malalties neurodegeneratives
Envelliment
Adenosine
Neurodegenerative Diseases
Aging
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spelling Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during AgingSánchez-Melgar, AlejandroAlbasanz, José LuisPallàs i Llibería, Mercè, 1964-Martín, MairenaAdenosinaMalalties neurodegenerativesEnvellimentAdenosineNeurodegenerative DiseasesAgingAdenosine is a neuromodulator that has been involved in aging and neurodegenerative diseases as Alzheimer's disease (AD). In the present work, we analyzed the possible modulation of purine metabolites, 5'nucleotidase (5′NT) and adenosine deaminase (ADA) activities, and adenosine monophosphate (AMP)-activated protein kinase (AMPK) and its phosphorylated form during aging in the cerebral cortex. Three murine models were used: senescence-accelerated mouse-resistant 1 (SAMR1, normal senescence), senescence-accelerated mouse-prone 8 (SAMP8, a model of AD), and the wild-type C57BL/6J (model of aging) mice strains. Glutamate and excitatory amino acid transporter 2 (EAAT2) levels were also measured in these animals. HPLC, Western blotting, and enzymatic activity evaluation were performed to this aim. 5′-Nucleotidase (5′NT) activity was decreased at six months and recovered at 12 months in SAMP8 while opposite effects were observed in SAMR1 at the same age, and no changes in C57BL/6J mice. ADA activity significantly decreased from 3 to 12 months in the SAMR1 mice strain, while a significant decrease from 6 to 12 months was observed in the SAMP8 mice strain. Regarding purine metabolites, xanthine and guanosine levels were increased at six months in SAMR1 without significant differences in SAMP8 mice. In C57BL/6J mice, inosine and xanthine were increased, while adenosine decreased, from 4 to 24 months. The AMPK level was decreased at six months in SAMP8 without significant changes nor in SAMR1 or C57BL/6J strains. Glutamate and EAAT2 levels were also modulated during aging. Our data show a different modulation of adenosine metabolism participants in the cerebral cortex of these animal models. Interestingly, the main differences between SAMR1 and SAMP8 mice were found at six months of age, SAMP8 being the most affected strain. As SAMP8 is an AD model, results suggest that adenosinergic metabolism is involved in the neurodegeneration of AD.Ivyspring International2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/171435Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms21197300International Journal of Medical Sciences, 2020, vol. 21, num. 19, p. 7300-7320https://doi.org/10.3390/ijms21197300cc-by-nc (c) Ivyspring International, 2020http://creativecommons.org/licenses/by-nc/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1714352026-05-27T06:46:51Z
dc.title.none.fl_str_mv Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
title Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
spellingShingle Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
Sánchez-Melgar, Alejandro
Adenosina
Malalties neurodegeneratives
Envelliment
Adenosine
Neurodegenerative Diseases
Aging
title_short Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
title_full Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
title_fullStr Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
title_full_unstemmed Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
title_sort Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging
dc.creator.none.fl_str_mv Sánchez-Melgar, Alejandro
Albasanz, José Luis
Pallàs i Llibería, Mercè, 1964-
Martín, Mairena
author Sánchez-Melgar, Alejandro
author_facet Sánchez-Melgar, Alejandro
Albasanz, José Luis
Pallàs i Llibería, Mercè, 1964-
Martín, Mairena
author_role author
author2 Albasanz, José Luis
Pallàs i Llibería, Mercè, 1964-
Martín, Mairena
author2_role author
author
author
dc.subject.none.fl_str_mv Adenosina
Malalties neurodegeneratives
Envelliment
Adenosine
Neurodegenerative Diseases
Aging
topic Adenosina
Malalties neurodegeneratives
Envelliment
Adenosine
Neurodegenerative Diseases
Aging
description Adenosine is a neuromodulator that has been involved in aging and neurodegenerative diseases as Alzheimer's disease (AD). In the present work, we analyzed the possible modulation of purine metabolites, 5'nucleotidase (5′NT) and adenosine deaminase (ADA) activities, and adenosine monophosphate (AMP)-activated protein kinase (AMPK) and its phosphorylated form during aging in the cerebral cortex. Three murine models were used: senescence-accelerated mouse-resistant 1 (SAMR1, normal senescence), senescence-accelerated mouse-prone 8 (SAMP8, a model of AD), and the wild-type C57BL/6J (model of aging) mice strains. Glutamate and excitatory amino acid transporter 2 (EAAT2) levels were also measured in these animals. HPLC, Western blotting, and enzymatic activity evaluation were performed to this aim. 5′-Nucleotidase (5′NT) activity was decreased at six months and recovered at 12 months in SAMP8 while opposite effects were observed in SAMR1 at the same age, and no changes in C57BL/6J mice. ADA activity significantly decreased from 3 to 12 months in the SAMR1 mice strain, while a significant decrease from 6 to 12 months was observed in the SAMP8 mice strain. Regarding purine metabolites, xanthine and guanosine levels were increased at six months in SAMR1 without significant differences in SAMP8 mice. In C57BL/6J mice, inosine and xanthine were increased, while adenosine decreased, from 4 to 24 months. The AMPK level was decreased at six months in SAMP8 without significant changes nor in SAMR1 or C57BL/6J strains. Glutamate and EAAT2 levels were also modulated during aging. Our data show a different modulation of adenosine metabolism participants in the cerebral cortex of these animal models. Interestingly, the main differences between SAMR1 and SAMP8 mice were found at six months of age, SAMP8 being the most affected strain. As SAMP8 is an AD model, results suggest that adenosinergic metabolism is involved in the neurodegeneration of AD.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/171435
url https://hdl.handle.net/2445/171435
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms21197300
International Journal of Medical Sciences, 2020, vol. 21, num. 19, p. 7300-7320
https://doi.org/10.3390/ijms21197300
dc.rights.none.fl_str_mv cc-by-nc (c) Ivyspring International, 2020
http://creativecommons.org/licenses/by-nc/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Ivyspring International, 2020
http://creativecommons.org/licenses/by-nc/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Ivyspring International
publisher.none.fl_str_mv Ivyspring International
dc.source.none.fl_str_mv Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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