Novel methodology for identification and quantification of microplastics in biological samples

Currently, the evidence of the ingestion of microplastics (MPs) by organisms or the accumulation in different environmental compartments has been achieved using several methodological procedures. However, its uses have not been standardized across studies. In this study, we aim to assess and validat...

ver descrição completa

Detalhes bibliográficos
Autores: Malafaia, Guilherme, Luz, Thiarlem Marinho da, Araújo, Amanda Pereira da Costa, Ahmed, Mohamed Ahmed Ibrahim, Rocha-Santos, Teresa, Barceló, Damià
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/309538
Acesso em linha:http://hdl.handle.net/10261/309538
https://api.elsevier.com/content/abstract/scopus_id/85118898599
Access Level:acceso abierto
Palavra-chave:Plastic pollution
Experimental protocol
Methods
Neubauer chamber
Plastic particles
http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
id ES_ab2cc9f02c4f355941e40cb4cc01d628
oai_identifier_str oai:digital.csic.es:10261/309538
network_acronym_str ES
network_name_str España
repository_id_str
spelling Novel methodology for identification and quantification of microplastics in biological samplesMalafaia, GuilhermeLuz, Thiarlem Marinho daAraújo, Amanda Pereira da CostaAhmed, Mohamed Ahmed IbrahimRocha-Santos, TeresaBarceló, DamiàPlastic pollutionExperimental protocolMethodsNeubauer chamberPlastic particleshttp://metadata.un.org/sdg/6Ensure availability and sustainable management of water and sanitation for allCurrently, the evidence of the ingestion of microplastics (MPs) by organisms or the accumulation in different environmental compartments has been achieved using several methodological procedures. However, its uses have not been standardized across studies. In this study, we aim to assess and validate a protocol that can be useful for optimizing the identification and quantification procedures of polyethylene microplastics (PE MPs) in biological samples. Initially, considering that numerous studies filter samples previously digested in cellulosic membranes for isolation and analysis of MPs, we evaluated whether washing these membranes with different reagents could contribute to the complete detachment of particles, as well as to their dispersion in the obtained solutions. However, none of the tested reagents (dimethyl sulfoxide, acetone, ethyl alcohol and chloroform), including purified water, was able to completely remove the MPs adhered to the membranes or facilitate their dispersion in the solutions. On the other hand, we observed that the digestion of the membranes by acetonitrile constituted a procedure that prevents the loss of particles due to adherence, in addition to promoting good dispersion of MPs. Subsequently, we evaluated the use of Neubauer chambers for the quantification of MPs, having observed a good recovery rate (>92%) and results with insignificant variation, in PE MPs solutions with different concentrations (0.15; 0.075 and 0.0375 mg/mL). Ultimately, the validation of the proposed procedures took place from the evaluation of the accumulation of PE MPs in Astyanax spp. juveniles, having demonstrated the efficiency and sensitivity of the method proposed for this purpose. Subsequently, our study provides a methodological alternative that can optimize MPs quantifications in biological samples and reduce the generation of biased or unreliable results.The authors are grateful to the Brazilian National Research Council (CNPq) (proc. N. 426531/2018–3) and to Goiano Federal Institute for the financial support. Malafaia G. holds productivity scholarship granted by CNPq (Proc. N. 307743/2018–7).Peer reviewedElsevier0000-0003-4773-75980000-0002-6968-32390000-0002-8873-0491Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/309538https://api.elsevier.com/content/abstract/scopus_id/85118898599reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésEnvironmental pollution (Barking, Essex : 1987)https://doi.org/10.1016/j.envpol.2021.118466Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3095382026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel methodology for identification and quantification of microplastics in biological samples
title Novel methodology for identification and quantification of microplastics in biological samples
spellingShingle Novel methodology for identification and quantification of microplastics in biological samples
Malafaia, Guilherme
Plastic pollution
Experimental protocol
Methods
Neubauer chamber
Plastic particles
http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
title_short Novel methodology for identification and quantification of microplastics in biological samples
title_full Novel methodology for identification and quantification of microplastics in biological samples
title_fullStr Novel methodology for identification and quantification of microplastics in biological samples
title_full_unstemmed Novel methodology for identification and quantification of microplastics in biological samples
title_sort Novel methodology for identification and quantification of microplastics in biological samples
dc.creator.none.fl_str_mv Malafaia, Guilherme
Luz, Thiarlem Marinho da
Araújo, Amanda Pereira da Costa
Ahmed, Mohamed Ahmed Ibrahim
Rocha-Santos, Teresa
Barceló, Damià
author Malafaia, Guilherme
author_facet Malafaia, Guilherme
Luz, Thiarlem Marinho da
Araújo, Amanda Pereira da Costa
Ahmed, Mohamed Ahmed Ibrahim
Rocha-Santos, Teresa
Barceló, Damià
author_role author
author2 Luz, Thiarlem Marinho da
Araújo, Amanda Pereira da Costa
Ahmed, Mohamed Ahmed Ibrahim
Rocha-Santos, Teresa
Barceló, Damià
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv 0000-0003-4773-7598
0000-0002-6968-3239
0000-0002-8873-0491
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Plastic pollution
Experimental protocol
Methods
Neubauer chamber
Plastic particles
http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
topic Plastic pollution
Experimental protocol
Methods
Neubauer chamber
Plastic particles
http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
description Currently, the evidence of the ingestion of microplastics (MPs) by organisms or the accumulation in different environmental compartments has been achieved using several methodological procedures. However, its uses have not been standardized across studies. In this study, we aim to assess and validate a protocol that can be useful for optimizing the identification and quantification procedures of polyethylene microplastics (PE MPs) in biological samples. Initially, considering that numerous studies filter samples previously digested in cellulosic membranes for isolation and analysis of MPs, we evaluated whether washing these membranes with different reagents could contribute to the complete detachment of particles, as well as to their dispersion in the obtained solutions. However, none of the tested reagents (dimethyl sulfoxide, acetone, ethyl alcohol and chloroform), including purified water, was able to completely remove the MPs adhered to the membranes or facilitate their dispersion in the solutions. On the other hand, we observed that the digestion of the membranes by acetonitrile constituted a procedure that prevents the loss of particles due to adherence, in addition to promoting good dispersion of MPs. Subsequently, we evaluated the use of Neubauer chambers for the quantification of MPs, having observed a good recovery rate (>92%) and results with insignificant variation, in PE MPs solutions with different concentrations (0.15; 0.075 and 0.0375 mg/mL). Ultimately, the validation of the proposed procedures took place from the evaluation of the accumulation of PE MPs in Astyanax spp. juveniles, having demonstrated the efficiency and sensitivity of the method proposed for this purpose. Subsequently, our study provides a methodological alternative that can optimize MPs quantifications in biological samples and reduce the generation of biased or unreliable results.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/309538
https://api.elsevier.com/content/abstract/scopus_id/85118898599
url http://hdl.handle.net/10261/309538
https://api.elsevier.com/content/abstract/scopus_id/85118898599
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Environmental pollution (Barking, Essex : 1987)
https://doi.org/10.1016/j.envpol.2021.118466
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416245981347840
score 15,811543

Registos relacionados