Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition

12 Pág.

Detalles Bibliográficos
Autores: Witzell, Johanna, Decker, Vicki Huizu Guo, Agostinelli, Marta, Romeralo, Carmen, Cleary, M., Albrectsen, Benedicte R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345446
Acceso en línea:http://hdl.handle.net/10261/345446
https://api.elsevier.com/content/abstract/scopus_id/85127982365
Access Level:acceso abierto
Palabra clave:Chrysomela tremula
Populus tremula
Condensed tannins
Fungal endophytes
Heterogeneity-diversity relationship hypothesis
Phenolics
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spelling Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen AdditionWitzell, JohannaDecker, Vicki Huizu GuoAgostinelli, MartaRomeralo, CarmenCleary, M.Albrectsen, Benedicte R.Chrysomela tremulaPopulus tremulaCondensed tanninsFungal endophytesHeterogeneity-diversity relationship hypothesisPhenolics12 Pág.Abiotic and biotic factors may shape the mycobiome communities in plants directly but also indirectly by modifying the quality of host plants as a substrate. We hypothesized that nitrogen fertilization (N) would determine the quality of aspen (Populus tremula) leaves as a substrate for the endophytic fungi, and that by subjecting the plants to N, we could manipulate the concentrations of positive (nutritious) and negative (antifungal) chemicals in leaves, thus changing the internal "chemical landscape" for the fungi. We expected that this would lead to changes in the fungal community composition, in line with the predictions of heterogeneity-diversity relationship and resource availability hypotheses. To test this, we conducted a greenhouse study where aspen plants were subjected to N treatment. The chemical status of the leaves was confirmed using GC/MS (114 metabolites, including amino acids and sugars), LC/MS (11 phenolics), and UV-spectrometry (antifungal condensed tannins, CTs), and the endophytic communities were characterized using culture-dependent sequencing. We found that N treatment reduced foliar concentrations of CT precursor catechin but not that of CTs. Nitrogen treatment also increased the concentrations of the amino acids and reduced the concentration of some sugars. We introduced beetle herbivores (H) as a second treatment but found no rapid changes in chemical traits nor strong effect on the diversity of endophytes induced by herbivores. A few rare fungi were associated with and potentially vectored by the beetle herbivores. Our findings indicate that in a controlled environment, the externally induced changes did not strongly alter endophyte diversity in aspen leaves.This research was supported by FORMAS, a Swedish Research Council for Sustainable Development (2012-01358 and 2016-00907) to JW and MA, The Royal Swedish Academy of Agriculture and Forestry to MA, MSCA-IF from the EU’s Horizon 2020 Research and Innovation Program (grant agreement No. 845419) to CR, and Swedish Strategic Research to BA. The work was further supported by the Swedish Metabolomics Centre and by the Berzelii Center at UPSC, Umeå.Peer reviewedFrontiers MediaSwedish Research Council for Sustainable DevelopmentRoyal Swedish Academy of SciencesEuropean CommissionSwedish Metabolomics CentreWitzell, Johanna [0000-0003-1741-443X]Decker, Vicki Huizu Guo [0000-0001-8007-2685]Agostinelli, Marta [0000-0001-9211-6518]Romeralo, Carmen [0000-0002-8510-9915]Albrectsen, Benedicte R. [0000-0002-9337-4540]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/345446https://api.elsevier.com/content/abstract/scopus_id/85127982365reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/845419Instituto de Ciencias Forestales (ICIFOR)https://doi.org/10.3389/fmicb.2022.846208Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3454462026-05-22T06:33:51Z
dc.title.none.fl_str_mv Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
title Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
spellingShingle Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
Witzell, Johanna
Chrysomela tremula
Populus tremula
Condensed tannins
Fungal endophytes
Heterogeneity-diversity relationship hypothesis
Phenolics
title_short Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
title_full Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
title_fullStr Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
title_full_unstemmed Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
title_sort Aspen Leaves as a "Chemical Landscape" for Fungal Endophyte Diversity-Effects of Nitrogen Addition
dc.creator.none.fl_str_mv Witzell, Johanna
Decker, Vicki Huizu Guo
Agostinelli, Marta
Romeralo, Carmen
Cleary, M.
Albrectsen, Benedicte R.
author Witzell, Johanna
author_facet Witzell, Johanna
Decker, Vicki Huizu Guo
Agostinelli, Marta
Romeralo, Carmen
Cleary, M.
Albrectsen, Benedicte R.
author_role author
author2 Decker, Vicki Huizu Guo
Agostinelli, Marta
Romeralo, Carmen
Cleary, M.
Albrectsen, Benedicte R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Swedish Research Council for Sustainable Development
Royal Swedish Academy of Sciences
European Commission
Swedish Metabolomics Centre
Witzell, Johanna [0000-0003-1741-443X]
Decker, Vicki Huizu Guo [0000-0001-8007-2685]
Agostinelli, Marta [0000-0001-9211-6518]
Romeralo, Carmen [0000-0002-8510-9915]
Albrectsen, Benedicte R. [0000-0002-9337-4540]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chrysomela tremula
Populus tremula
Condensed tannins
Fungal endophytes
Heterogeneity-diversity relationship hypothesis
Phenolics
topic Chrysomela tremula
Populus tremula
Condensed tannins
Fungal endophytes
Heterogeneity-diversity relationship hypothesis
Phenolics
description 12 Pág.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345446
https://api.elsevier.com/content/abstract/scopus_id/85127982365
url http://hdl.handle.net/10261/345446
https://api.elsevier.com/content/abstract/scopus_id/85127982365
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/845419
Instituto de Ciencias Forestales (ICIFOR)
https://doi.org/10.3389/fmicb.2022.846208
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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