Heme oxygenase-1 inducer hemin does not inhibit SARS-CoV-2 virus infection

Antiviral agents with different mechanisms of action could induce synergistic effects against SARS-CoV-2 infection. Some reports suggest the therapeutic potential of the heme oxygenase-1 (HO-1) enzyme against virus infection. Given that hemin is a natural inducer of the HO-1 gene, the aim of this st...

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Detalhes bibliográficos
Autores: Maestro, S. (Sheila)|||/items/0cab4b65-d17d-4a9b-b718-41aed6636ec3, Córdoba, K.M. (Karol M.)|||/items/6251ff41-a22c-4162-9aea-fe6320d7d03c, Olagüe, C. (Cristina)|||/items/741bf268-0262-472c-bba3-5d9c1c380c44, Argemí, J. (Josepmaria)|||/items/cc37a6af-13a2-4fe0-846c-4725cca0ae72, Avila, M.A. (Matías Antonio)|||/items/3ad9abbb-c18d-445b-86cf-cb76be15419f, González-Aseguinolaza, G. (Gloria)|||/items/cb28732d-02bf-4339-ab60-ff738ee191ac, Smerdou-Picazo, C. (Cristian)|||/items/48fd1bd4-6483-45c9-9951-8a1b04873227, Fontanellas-Romá, A. (Antonio)|||/items/4ca7871d-480e-4841-b383-6a0da85f3a81
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universidad de Navarra
Repositório:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglês
OAI Identifier:oai:dadun.unav.edu:10171/115008
Acesso em linha:https://hdl.handle.net/10171/115008
Access Level:Acceso aberto
Palavra-chave:Antiviral effect
Heme oxygenase-1 induction
Hemin
SARS-CoV-2 infection
Descrição
Resumo:Antiviral agents with different mechanisms of action could induce synergistic effects against SARS-CoV-2 infection. Some reports suggest the therapeutic potential of the heme oxygenase-1 (HO-1) enzyme against virus infection. Given that hemin is a natural inducer of the HO-1 gene, the aim of this study was to develop an in vitro assay to analyze the antiviral potency of hemin against SARS-CoV-2 infection. A SARS-CoV-2 infectivity assay was conducted in Vero-E6 and Calu-3 epithelial cell lines. The antiviral effect of hemin, and chloroquine as a control, against SARS-CoV-2 virus infection was quantified by RT-qPCR using specific oligonucleotides for the N gene. Chloroquine induced a marked reduction of viral genome copies in kidney epithelial Vero-E6 cells but not in lung cancer Calu-3 cells. Hemin administration to the culture medium induced a high induction in the expression of the HO-1 gene that was stronger in Vero-E6 macaque-derived cells than in the human Calu-3 cell line. However, hemin treatment did not modify SARS-CoV-2 replication, as measured by viral genome quantification 48 h post-infection for Vero-E6 and 72 h post-infection for the Calu-3 lineages. In conclusion, although exposure to hemin induced strong HO-1 up-regulation, this effect was unable to inhibit or delay the progression of SARS-CoV-2 infection in two epithelial cell lines susceptible to infection.

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