Quantitative comparison of bacterial communities in two Mediterranean sponges

5 páginas.

Detalles Bibliográficos
Autores: Noyer, Charlotte, Hamilton, A., Sacristán-Soriano, Oriol, Becerro, Mikel
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/56422
Acceso en línea:http://hdl.handle.net/10261/56422
Access Level:acceso abierto
Palabra clave:Aplysina aerophoba
Symbiosis
Porifera
Real time PCR
Sponge-associated bacteria
Spongia agaricina-lamella
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spelling Quantitative comparison of bacterial communities in two Mediterranean spongesNoyer, CharlotteHamilton, A.Sacristán-Soriano, OriolBecerro, MikelAplysina aerophobaSymbiosisPoriferaReal time PCRSponge-associated bacteriaSpongia agaricina-lamella5 páginas.Marine sponges can host in their tissues abundant and diverse bacterial communities. Lack of truly quantitative data on bacterial abundance and dynamics limits our understanding of the organization and functioning of these endobiotic communities. In this technical note, we describe a quantitative polymerase chain reaction approach to quantify the relative abundance of multiple clades of three major sponge-associated bacterial phyla: Chloroflexi, Acidobacteria, and Actinobacteria. To test our approach we used the Mediterranean sponges Spongia lamella and Aplysina aerophoba. We designed five out of the six primer sets used in our study. We tested the new primer sets for specificity and optimized their conditions. Our preliminary data showed that Spongia lamella had larger bacterial abundance than Aplysina aerophoba, except for one clade of Chloroflexi. The two Chloroflexi clades investigated in our study amplified a fraction of the Chloroflexi present in Spongia lamella and most of what is present in Aplysina aerophoba, suggesting a more diverse Chloroflexi population in Spongia lamella than in Aplysina aerophoba. This quantitative technique has a great potential to provide a rapid and robust assessment of sponge microbial target and could contribute to deciphering the complexity of these largely unknown host-symbiont interactions.Peer reviewedSpringer Nature201220122010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/56422reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s13199-010-0082-2info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/564222026-05-22T06:33:51Z
dc.title.none.fl_str_mv Quantitative comparison of bacterial communities in two Mediterranean sponges
title Quantitative comparison of bacterial communities in two Mediterranean sponges
spellingShingle Quantitative comparison of bacterial communities in two Mediterranean sponges
Noyer, Charlotte
Aplysina aerophoba
Symbiosis
Porifera
Real time PCR
Sponge-associated bacteria
Spongia agaricina-lamella
title_short Quantitative comparison of bacterial communities in two Mediterranean sponges
title_full Quantitative comparison of bacterial communities in two Mediterranean sponges
title_fullStr Quantitative comparison of bacterial communities in two Mediterranean sponges
title_full_unstemmed Quantitative comparison of bacterial communities in two Mediterranean sponges
title_sort Quantitative comparison of bacterial communities in two Mediterranean sponges
dc.creator.none.fl_str_mv Noyer, Charlotte
Hamilton, A.
Sacristán-Soriano, Oriol
Becerro, Mikel
author Noyer, Charlotte
author_facet Noyer, Charlotte
Hamilton, A.
Sacristán-Soriano, Oriol
Becerro, Mikel
author_role author
author2 Hamilton, A.
Sacristán-Soriano, Oriol
Becerro, Mikel
author2_role author
author
author
dc.subject.none.fl_str_mv Aplysina aerophoba
Symbiosis
Porifera
Real time PCR
Sponge-associated bacteria
Spongia agaricina-lamella
topic Aplysina aerophoba
Symbiosis
Porifera
Real time PCR
Sponge-associated bacteria
Spongia agaricina-lamella
description 5 páginas.
publishDate 2010
dc.date.none.fl_str_mv 2010
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/56422
url http://hdl.handle.net/10261/56422
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s13199-010-0082-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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