In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model

13 pages, 8 figures.-- Under a Creative Commons license

Detalles Bibliográficos
Autores: Diogo, Gabriela S., Permuy, María, Marques, Catarina F., González Sotelo, Carmen, Pérez Martín, Ricardo Isaac, Serra, Julia, González, Pio, Muñoz, Fernando, Pirraco, Rogério P., Reis, Rui L., Silva, Tiago H.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/350682
Acceso en línea:http://hdl.handle.net/10261/350682
Access Level:acceso abierto
Palabra clave:Fish collagen
Apatite
Blue shark
Bone regeneration
New Zealand rabbit
Marine biomaterials
http://aims.fao.org/aos/agrovoc/c_1755
http://aims.fao.org/aos/agrovoc/c_5bbf486b
http://aims.fao.org/aos/agrovoc/c_7028
collagen
apatite
sharks
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oai_identifier_str oai:digital.csic.es:10261/350682
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spelling In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit modelDiogo, Gabriela S.Permuy, MaríaMarques, Catarina F.González Sotelo, CarmenPérez Martín, Ricardo IsaacSerra, JuliaGonzález, PioMuñoz, FernandoPirraco, Rogério P.Reis, Rui L.Silva, Tiago H.Fish collagenApatiteBlue sharkBone regenerationNew Zealand rabbitMarine biomaterialshttp://aims.fao.org/aos/agrovoc/c_1755http://aims.fao.org/aos/agrovoc/c_5bbf486bhttp://aims.fao.org/aos/agrovoc/c_7028collagenapatitesharks13 pages, 8 figures.-- Under a Creative Commons licenseThe ability of human tissues to self-repair is limited, which motivates the scientific community to explore new and better therapeutic approaches to tissue regeneration. The present manuscript provides a comparative study between a marine-based composite biomaterial, and another composed of well-established counterparts for bone tissue regeneration. Blue shark skin collagen was combined with bioapatite obtained from blue shark's teeth (mColl:BAp), while bovine collagen was combined with synthetic hydroxyapatite (bColl:Ap) to produce 3D composite scaffolds by freeze-drying. Collagens showed similar profiles, while apatite particles differed in their composition, being the marine bioapatite a fluoride-enriched ceramic. The marine-sourced biomaterials presented higher porosities, improved mechanical properties, and slower degradation rates when compared to synthetic apatite-reinforced bovine collagen. The in vivo performance regarding bone tissue regeneration was evaluated in defects created in femoral condyles in New Zealand rabbits twelve weeks post-surgery. Micro-CT results showed that mColl:BAp implanted condyles had a slower degradation and an higher tissue formation (17.9 ± 6.9 %) when compared with bColl:Ap implanted ones (12.9 ± 7.6 %). The histomorphometry analysis provided supporting evidence, confirming the observed trend by quantifying 13.1 ± 7.9 % of new tissue formation for mColl:BAp composites and 10.4 ± 3.2 % for bColl:Ap composites, suggesting the potential use of marine biomaterials for bone regenerationThis study was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the BiogenInk project (M-ERA-NET2/0022/2016), by European Regional Development Fund through INTERREG Spain-Portugal Programme, under the scope of 0245_IBEROS_1_E project, through INTERREG Atlantic Area Programme, under the scope of BLUEHUMAN (EAPA_151/2016) project and through Northern Portugal Regional Operational Program (NORTE 2020), under the scope of Structured project NORTE-01-0145-FEDER-000021. The Doctoral Programme NORTE-08-5369-FSE-000044 supported by NORTE 2020, under the PORTUGAL 2020 Partnership Agreement, through the European Social Fund, is also greatly acknowledged by the PhD fellowship of GSD. RP and CM thank FCT for the research contracts IF/00347/2015 and CEECIND/04687/2017, respectivelyPeer reviewedElsevierFundação para a Ciência e a Tecnologia (Portugal)European CommissionDiogo, Gabriela S. [0000-0002-5696-631X]Permuy, María [0000-0002-3708-4821]González Sotelo, Carmen [0000-0002-0726-4370]Pérez Martín, Ricardo Isaac [0000-0002-4918-9399]Muñoz, Fernando [0000-0002-4130-1526]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/350682reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésNo data was used for the research described in the articlehttps://doi.org/10.1016/j.bioadv.2024.213813Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3506822026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
title In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
spellingShingle In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
Diogo, Gabriela S.
Fish collagen
Apatite
Blue shark
Bone regeneration
New Zealand rabbit
Marine biomaterials
http://aims.fao.org/aos/agrovoc/c_1755
http://aims.fao.org/aos/agrovoc/c_5bbf486b
http://aims.fao.org/aos/agrovoc/c_7028
collagen
apatite
sharks
title_short In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
title_full In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
title_fullStr In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
title_full_unstemmed In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
title_sort In vivo assessment of marine vs bovine origin collagen-based composite scaffolds promoting bone regeneration in a New Zealand rabbit model
dc.creator.none.fl_str_mv Diogo, Gabriela S.
Permuy, María
Marques, Catarina F.
González Sotelo, Carmen
Pérez Martín, Ricardo Isaac
Serra, Julia
González, Pio
Muñoz, Fernando
Pirraco, Rogério P.
Reis, Rui L.
Silva, Tiago H.
author Diogo, Gabriela S.
author_facet Diogo, Gabriela S.
Permuy, María
Marques, Catarina F.
González Sotelo, Carmen
Pérez Martín, Ricardo Isaac
Serra, Julia
González, Pio
Muñoz, Fernando
Pirraco, Rogério P.
Reis, Rui L.
Silva, Tiago H.
author_role author
author2 Permuy, María
Marques, Catarina F.
González Sotelo, Carmen
Pérez Martín, Ricardo Isaac
Serra, Julia
González, Pio
Muñoz, Fernando
Pirraco, Rogério P.
Reis, Rui L.
Silva, Tiago H.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
Diogo, Gabriela S. [0000-0002-5696-631X]
Permuy, María [0000-0002-3708-4821]
González Sotelo, Carmen [0000-0002-0726-4370]
Pérez Martín, Ricardo Isaac [0000-0002-4918-9399]
Muñoz, Fernando [0000-0002-4130-1526]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fish collagen
Apatite
Blue shark
Bone regeneration
New Zealand rabbit
Marine biomaterials
http://aims.fao.org/aos/agrovoc/c_1755
http://aims.fao.org/aos/agrovoc/c_5bbf486b
http://aims.fao.org/aos/agrovoc/c_7028
collagen
apatite
sharks
topic Fish collagen
Apatite
Blue shark
Bone regeneration
New Zealand rabbit
Marine biomaterials
http://aims.fao.org/aos/agrovoc/c_1755
http://aims.fao.org/aos/agrovoc/c_5bbf486b
http://aims.fao.org/aos/agrovoc/c_7028
collagen
apatite
sharks
description 13 pages, 8 figures.-- Under a Creative Commons license
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/350682
url http://hdl.handle.net/10261/350682
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv No data was used for the research described in the article
https://doi.org/10.1016/j.bioadv.2024.213813

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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