Modeling survival curves of Anisakis L3 after isothermal heat treatments at lethal temperatures
Even though anisakiasis is considered, nowadays, a significant threat to public health all over the world, no attempt has been made up to date to mathematically describe the thermal susceptibility of Anisakis larvae in the third stage (L3). To fill this gap, in this paper, more than 10,000 free (non...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/334653 |
| Acceso en línea: | http://hdl.handle.net/10261/334653 |
| Access Level: | acceso abierto |
| Palabra clave: | Anisakis larvae Isothermal survival curves Thermal inactivation Modeling Fish cooking Food safety |
| Sumario: | Even though anisakiasis is considered, nowadays, a significant threat to public health all over the world, no attempt has been made up to date to mathematically describe the thermal susceptibility of Anisakis larvae in the third stage (L3). To fill this gap, in this paper, more than 10,000 free (non-encysted) Anisakis L3 were individually heat treated in a thermal cycler at temperatures between 44 °C and 61 °C for different exposure times. After heat exposition, viability was assessed in each larva, survival curves at isothermal conditions were derived, and the effectiveness of four kinetic models (fundamental kinetic model, Mafart model, and probit and logit models) in describing these curves was tested. Evaluation of larvae viability after heat exposition revealed sigmoidal survival curves that increased their steepness with temperature. Of the four models tested, the Mafart model was the one that best fitted the data only differing from the observed survival ratios by 0.12 units on average. Validation experiments performed at temperatures different to those used to create the model corroborated its predictive capacity. Future efforts should be focused in predicting larvae viability at non-isothermal conditions as those occurring during fish cooking. |
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