Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula

The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations re...

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Autores: Vázquez-García, José G., Rojano-Delgado, Antonia M., Alcántara-de la Cruz, Ricardo, Torra Farré, Joel, Dellaferrera, Ignacio, Portugal, João M., Prado, Rafael de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/70739
Acceso en línea:https://doi.org/10.3389/fpls.2021.617040
http://hdl.handle.net/10459.1/70739
Access Level:acceso abierto
Palabra clave:Barnyard grass
Enhanced metabolism
Glyphosate
Non-target-site resistance (NTSR)
Resistance mechanisms
Target-site resistance (TSR)
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spelling Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian PeninsulaVázquez-García, José G.Rojano-Delgado, Antonia M.Alcántara-de la Cruz, RicardoTorra Farré, JoelDellaferrera, IgnacioPortugal, João M.Prado, Rafael deBarnyard grassEnhanced metabolismGlyphosateNon-target-site resistance (NTSR)Resistance mechanismsTarget-site resistance (TSR)The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.This work was funded by the Asociacion de Agroquímicos y Medioambiente and by national funds through the Fundação para a Ciência e Tecnologia, I.P. by the project UIDB/05064/2020 (VALORIZA).Frontiers Media2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3389/fpls.2021.617040http://hdl.handle.net/10459.1/70739reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.3389/fpls.2021.617040Frontiers in Plant Science, 2021, vol. 12, article 617040cc-by (c) Vázquez-García et al., 2021info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/707392026-06-24T12:42:17Z
dc.title.none.fl_str_mv Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
spellingShingle Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
Vázquez-García, José G.
Barnyard grass
Enhanced metabolism
Glyphosate
Non-target-site resistance (NTSR)
Resistance mechanisms
Target-site resistance (TSR)
title_short Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_full Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_fullStr Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_full_unstemmed Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
title_sort Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
dc.creator.none.fl_str_mv Vázquez-García, José G.
Rojano-Delgado, Antonia M.
Alcántara-de la Cruz, Ricardo
Torra Farré, Joel
Dellaferrera, Ignacio
Portugal, João M.
Prado, Rafael de
author Vázquez-García, José G.
author_facet Vázquez-García, José G.
Rojano-Delgado, Antonia M.
Alcántara-de la Cruz, Ricardo
Torra Farré, Joel
Dellaferrera, Ignacio
Portugal, João M.
Prado, Rafael de
author_role author
author2 Rojano-Delgado, Antonia M.
Alcántara-de la Cruz, Ricardo
Torra Farré, Joel
Dellaferrera, Ignacio
Portugal, João M.
Prado, Rafael de
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Barnyard grass
Enhanced metabolism
Glyphosate
Non-target-site resistance (NTSR)
Resistance mechanisms
Target-site resistance (TSR)
topic Barnyard grass
Enhanced metabolism
Glyphosate
Non-target-site resistance (NTSR)
Resistance mechanisms
Target-site resistance (TSR)
description The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fpls.2021.617040
http://hdl.handle.net/10459.1/70739
url https://doi.org/10.3389/fpls.2021.617040
http://hdl.handle.net/10459.1/70739
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fpls.2021.617040
Frontiers in Plant Science, 2021, vol. 12, article 617040
dc.rights.none.fl_str_mv cc-by (c) Vázquez-García et al., 2021
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Vázquez-García et al., 2021
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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