A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells

In bovine chromaffin cells fast-superfused with Krebs-HEPES solution containing 1–2 mM Ca2+, 5 s pulses of choline (1–10 mM), elicited catecholamine secretory responses that were only ~10% of those evoked by ACh (0.01–0.1 mM). However, in high-Ca2+ solutions (10–20 mM) the size of the choline secret...

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Autores: Fuentealba, Jorge, Olivares, Román, Alés, Eva, Tapia, Laura, Rojo, Jonathan, Arroyo, Gloria, Aldea, Marcos, Criado Herrero, Manuel, Gandía, Luis, García, Antonio G.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2004
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/288387
Acceso en línea:http://hdl.handle.net/10261/288387
Access Level:acceso abierto
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spelling A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cellsFuentealba, JorgeOlivares, RománAlés, EvaTapia, LauraRojo, JonathanArroyo, GloriaAldea, MarcosCriado Herrero, ManuelGandía, LuisGarcía, Antonio G.In bovine chromaffin cells fast-superfused with Krebs-HEPES solution containing 1–2 mM Ca2+, 5 s pulses of choline (1–10 mM), elicited catecholamine secretory responses that were only ~10% of those evoked by ACh (0.01–0.1 mM). However, in high-Ca2+ solutions (10–20 mM) the size of the choline secretory responses approached those of ACh. The choline responses (10 mM choline in 20 mM Ca2+, 10Cho/20Ca2+) tended to decline upon repetitive pulsing, whereas those of ACh were well maintained. The confocal [Ca2+]c increases evoked by 10Cho/20Ca2+ were similar to those of ACh. Whereas 10Cho/20Ca2+ caused mostly hyperpolarization of chromaffin cells, 0.1ACh/20 Ca2+ caused first depolarization and then hyperpolarization; in regular solutions (2 mM Ca2+), the hyperpolarizing responses did not show up. In Xenopus oocytes injected with mRNA for bovine α7 nicotinic receptors (nAChRs), 10Cho/20 Ca2+ fully activated an inward current; in oocytes expressing α3β4, however, the inward current elicited by choline amounted to only 4% of the size of α7 current. Our results suggest that choline activates the entry of Ca2+ through α7 nAChRs; this leads to a cytosolic concentration of calcium ([Ca2+]c) rise that causes the activation of nearby Ca2+-dependent K+ channels and the hyperpolarization of the chromaffin cell. This response, which could be unmasked provided that cells were stimulated with high-Ca2+ solutions, may be the underlying mechanism through which choline exerts a modulatory effect on the electrical activity of the chromaffin cell and on neurotransmitter release at cholinergic synapses.This study has been supported by grants to A.G.G. from Programa III PRICIT grupos estratégicos de la Comunidad de Madrid/UAM; DGICYT Nº PM99-0005 and PM99-0004; FIS No. 01/0183, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo; MCYT BFI 2003- 02722; Cartonajes La Huerta, Molina de Segura, Murcia; Spain; and Fundación La Caixa, Barcelona, Spain. M.C. was supported by grants from the Spanish Ministry of Science and Technology, BMC2002-00972, and Generalitat Valenciana, CTIDIB/2002/138 and GRUPOS03/038. J.F is a fellow of MECD, Spain, and is also supported by Fundación Teófilo Hernando, Spain.Peer reviewedFederation of American Societies for Experimental BiologyJohn Wiley & SonsDirección General de Investigación Científica y Técnica, DGICT (España)Instituto de Salud Carlos IIIComunidad de MadridMinisterio de Ciencia y Tecnología (España)Ministerio de Sanidad y Consumo (España)Fundación la CaixaGeneralitat ValencianaMinisterio de Educación, Cultura y Deporte (España)Instituto Fundación Teófilo HernandoConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232004info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/288387reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésFASEB Journalhttps://doi.org/10.1096/fj.04-1828fjeSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2883872026-05-22T06:33:51Z
dc.title.none.fl_str_mv A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
title A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
spellingShingle A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
Fuentealba, Jorge
title_short A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
title_full A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
title_fullStr A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
title_full_unstemmed A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
title_sort A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells
dc.creator.none.fl_str_mv Fuentealba, Jorge
Olivares, Román
Alés, Eva
Tapia, Laura
Rojo, Jonathan
Arroyo, Gloria
Aldea, Marcos
Criado Herrero, Manuel
Gandía, Luis
García, Antonio G.
author Fuentealba, Jorge
author_facet Fuentealba, Jorge
Olivares, Román
Alés, Eva
Tapia, Laura
Rojo, Jonathan
Arroyo, Gloria
Aldea, Marcos
Criado Herrero, Manuel
Gandía, Luis
García, Antonio G.
author_role author
author2 Olivares, Román
Alés, Eva
Tapia, Laura
Rojo, Jonathan
Arroyo, Gloria
Aldea, Marcos
Criado Herrero, Manuel
Gandía, Luis
García, Antonio G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dirección General de Investigación Científica y Técnica, DGICT (España)
Instituto de Salud Carlos III
Comunidad de Madrid
Ministerio de Ciencia y Tecnología (España)
Ministerio de Sanidad y Consumo (España)
Fundación la Caixa
Generalitat Valenciana
Ministerio de Educación, Cultura y Deporte (España)
Instituto Fundación Teófilo Hernando
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description In bovine chromaffin cells fast-superfused with Krebs-HEPES solution containing 1–2 mM Ca2+, 5 s pulses of choline (1–10 mM), elicited catecholamine secretory responses that were only ~10% of those evoked by ACh (0.01–0.1 mM). However, in high-Ca2+ solutions (10–20 mM) the size of the choline secretory responses approached those of ACh. The choline responses (10 mM choline in 20 mM Ca2+, 10Cho/20Ca2+) tended to decline upon repetitive pulsing, whereas those of ACh were well maintained. The confocal [Ca2+]c increases evoked by 10Cho/20Ca2+ were similar to those of ACh. Whereas 10Cho/20Ca2+ caused mostly hyperpolarization of chromaffin cells, 0.1ACh/20 Ca2+ caused first depolarization and then hyperpolarization; in regular solutions (2 mM Ca2+), the hyperpolarizing responses did not show up. In Xenopus oocytes injected with mRNA for bovine α7 nicotinic receptors (nAChRs), 10Cho/20 Ca2+ fully activated an inward current; in oocytes expressing α3β4, however, the inward current elicited by choline amounted to only 4% of the size of α7 current. Our results suggest that choline activates the entry of Ca2+ through α7 nAChRs; this leads to a cytosolic concentration of calcium ([Ca2+]c) rise that causes the activation of nearby Ca2+-dependent K+ channels and the hyperpolarization of the chromaffin cell. This response, which could be unmasked provided that cells were stimulated with high-Ca2+ solutions, may be the underlying mechanism through which choline exerts a modulatory effect on the electrical activity of the chromaffin cell and on neurotransmitter release at cholinergic synapses.
publishDate 2004
dc.date.none.fl_str_mv 2004
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/288387
url http://hdl.handle.net/10261/288387
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv FASEB Journal
https://doi.org/10.1096/fj.04-1828fje
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
John Wiley & Sons
publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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