Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)

A mapping F2 population from the cross ‘Piel de Sapo’ × PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Sh...

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Autores: Díaz, Aurora, Zarouri, Belkacem, Fergany, Mohamed, Eduardo, Iban, Álvarez, José María, Picó-Silvent, Belén, Monforte, Antonio J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/249217
Acceso en línea:http://hdl.handle.net/10261/249217
Access Level:acceso abierto
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spelling Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)Díaz, AuroraZarouri, BelkacemFergany, MohamedEduardo, IbanÁlvarez, José MaríaPicó-Silvent, BelénMonforte, Antonio J.A mapping F2 population from the cross ‘Piel de Sapo’ × PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in ‘Piel de Sapo’ background which yields round melons.This work was supported by grants AGL2009-12698-C02-02 and AGL2012-40130-C02-02 from the Spanish Ministry of Economy and Competitiveness to AJM. AD was supported by a JAE-Doc contract from CSIC, MF by a Postdoctoral contract from GRAG, IE by a fellowship from the former Spanish Ministry of Education and BZ by a fellowship from Instituto Agronómico Mediterráneo de Zaragoza (IAMZ), Spain.Peer reviewedPublic Library of ScienceMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Instituto Agronómico Mediterráneo de ZaragozaMinisterio de Educación (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/249217reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1371/journal.pone.0104188Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2492172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
title Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
spellingShingle Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
Díaz, Aurora
title_short Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
title_full Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
title_fullStr Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
title_full_unstemmed Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
title_sort Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucucumis melo L.)
dc.creator.none.fl_str_mv Díaz, Aurora
Zarouri, Belkacem
Fergany, Mohamed
Eduardo, Iban
Álvarez, José María
Picó-Silvent, Belén
Monforte, Antonio J.
author Díaz, Aurora
author_facet Díaz, Aurora
Zarouri, Belkacem
Fergany, Mohamed
Eduardo, Iban
Álvarez, José María
Picó-Silvent, Belén
Monforte, Antonio J.
author_role author
author2 Zarouri, Belkacem
Fergany, Mohamed
Eduardo, Iban
Álvarez, José María
Picó-Silvent, Belén
Monforte, Antonio J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Instituto Agronómico Mediterráneo de Zaragoza
Ministerio de Educación (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description A mapping F2 population from the cross ‘Piel de Sapo’ × PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in ‘Piel de Sapo’ background which yields round melons.
publishDate 2014
dc.date.none.fl_str_mv 2014
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/249217
url http://hdl.handle.net/10261/249217
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1371/journal.pone.0104188
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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