L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression

Background and objective: altered adhesive interaction between bone marrow (BM) stroma and progenitors in chronic myeloid leukemia (CML) may be in part caused by abnormal expression of cell adhesion molecules (CAMs) on malignant progenitor cells. Treatment of CML with interferon-a (IFN-a) re-establi...

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Detalhes bibliográficos
Autores: Martin-Henao, Gregorio, Quiroga, Regina, Sureda, Anna, González Ruiz, Juan Ramón, Moreno Aguado, Víctor, García, Juan
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/172476
Acesso em linha:https://hdl.handle.net/2445/172476
Access Level:acceso abierto
Palavra-chave:Farmacologia
Hematopoesi
Metabolisme
Interferó
Biosíntesi
Pharmacology
Hematopoiesis
Metabolism
Interferon
Biosynthesis
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repository_id_str
spelling L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expressionMartin-Henao, GregorioQuiroga, ReginaSureda, AnnaGonzález Ruiz, Juan RamónMoreno Aguado, VíctorGarcía, JuanFarmacologiaHematopoesiMetabolismeInterferóBiosíntesiPharmacologyHematopoiesisMetabolismInterferonBiosynthesisBackground and objective: altered adhesive interaction between bone marrow (BM) stroma and progenitors in chronic myeloid leukemia (CML) may be in part caused by abnormal expression of cell adhesion molecules (CAMs) on malignant progenitor cells. Treatment of CML with interferon-a (IFN-a) re-establishes normal hemopoiesis in some patients in part by restoring normal adhesive interactions between CML progenitors and BM microenvironment, which may in turn be mediated by correcting CAM expression on progenitors. Design and methods: we investigated the expression of CAMs (L-selectin, b((2))-integrin, LFA-3, ICAM-1, ICAM-3, NCAM) on purified BM CD34(+) cells from CML patients (n= 34) and healthy adults (n= 15) by flow cytometry. Modulation of L-selectin expression on CD34(+) cells from CML after in vitro treatment with IFN-a was also investigated. RESULTS: The mean percentage of CD34(+ )cells expressing L-selectin was significantly lower in CML patients (25.4+/-12.8%) than in normal controls (68.7+/-8.3%, n=15). CD34(+)/HLA-DR(-/low) and CD34(+)/ CD38(-/low) co-expressing L-selectin were also significantly lower in untreated CML (27.4+/-21.5% and 39.8+/-26.7%, respectively, n=8) than in controls (61+/-17% and 83.7+/-10%, respectively, n=7). In vitro treatment with IFN-a of purified CD34(+) BM cells from untreated CML patients (n=8) induced a significant, dose and time-dependent increase in the L-selectin expression as indicated by FACS analysis. Interpretation and conclusions: we hypothesize that this L-selectin deficiency reflects a cell surface adhesion defect of progenitors from CML that is partially restored by in vitro IFN-a treatment. These data may help to explain the adhesive abnormalities of CML progenitors to the BM microenvironment and the in vitro restoration of adhesion capacity after IFN-a treatment.Ferrata Storti Foundation2020202020002020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/172476Articles publicats en revistes (Ciències Clíniques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://haematologica.org/issue/view/66Haematologica, 2000, vol. 85, num. 2, p. 139-146(c) Ferrata Storti Foundation, 2000info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1724762026-05-29T05:05:01Z
dc.title.none.fl_str_mv L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
title L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
spellingShingle L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
Martin-Henao, Gregorio
Farmacologia
Hematopoesi
Metabolisme
Interferó
Biosíntesi
Pharmacology
Hematopoiesis
Metabolism
Interferon
Biosynthesis
title_short L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
title_full L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
title_fullStr L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
title_full_unstemmed L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
title_sort L-selectin expression is low on CD34 + cells from patients with chronic myeloid leukemia and interferon-a up-regulates this expression
dc.creator.none.fl_str_mv Martin-Henao, Gregorio
Quiroga, Regina
Sureda, Anna
González Ruiz, Juan Ramón
Moreno Aguado, Víctor
García, Juan
author Martin-Henao, Gregorio
author_facet Martin-Henao, Gregorio
Quiroga, Regina
Sureda, Anna
González Ruiz, Juan Ramón
Moreno Aguado, Víctor
García, Juan
author_role author
author2 Quiroga, Regina
Sureda, Anna
González Ruiz, Juan Ramón
Moreno Aguado, Víctor
García, Juan
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Farmacologia
Hematopoesi
Metabolisme
Interferó
Biosíntesi
Pharmacology
Hematopoiesis
Metabolism
Interferon
Biosynthesis
topic Farmacologia
Hematopoesi
Metabolisme
Interferó
Biosíntesi
Pharmacology
Hematopoiesis
Metabolism
Interferon
Biosynthesis
description Background and objective: altered adhesive interaction between bone marrow (BM) stroma and progenitors in chronic myeloid leukemia (CML) may be in part caused by abnormal expression of cell adhesion molecules (CAMs) on malignant progenitor cells. Treatment of CML with interferon-a (IFN-a) re-establishes normal hemopoiesis in some patients in part by restoring normal adhesive interactions between CML progenitors and BM microenvironment, which may in turn be mediated by correcting CAM expression on progenitors. Design and methods: we investigated the expression of CAMs (L-selectin, b((2))-integrin, LFA-3, ICAM-1, ICAM-3, NCAM) on purified BM CD34(+) cells from CML patients (n= 34) and healthy adults (n= 15) by flow cytometry. Modulation of L-selectin expression on CD34(+) cells from CML after in vitro treatment with IFN-a was also investigated. RESULTS: The mean percentage of CD34(+ )cells expressing L-selectin was significantly lower in CML patients (25.4+/-12.8%) than in normal controls (68.7+/-8.3%, n=15). CD34(+)/HLA-DR(-/low) and CD34(+)/ CD38(-/low) co-expressing L-selectin were also significantly lower in untreated CML (27.4+/-21.5% and 39.8+/-26.7%, respectively, n=8) than in controls (61+/-17% and 83.7+/-10%, respectively, n=7). In vitro treatment with IFN-a of purified CD34(+) BM cells from untreated CML patients (n=8) induced a significant, dose and time-dependent increase in the L-selectin expression as indicated by FACS analysis. Interpretation and conclusions: we hypothesize that this L-selectin deficiency reflects a cell surface adhesion defect of progenitors from CML that is partially restored by in vitro IFN-a treatment. These data may help to explain the adhesive abnormalities of CML progenitors to the BM microenvironment and the in vitro restoration of adhesion capacity after IFN-a treatment.
publishDate 2000
dc.date.none.fl_str_mv 2000
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/172476
url https://hdl.handle.net/2445/172476
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://haematologica.org/issue/view/66
Haematologica, 2000, vol. 85, num. 2, p. 139-146
dc.rights.none.fl_str_mv (c) Ferrata Storti Foundation, 2000
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Ferrata Storti Foundation, 2000
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Ferrata Storti Foundation
publisher.none.fl_str_mv Ferrata Storti Foundation
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416195649699840
score 15,812429

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