Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors

The large-scale continuous production of niosomes remains challenging. The inherent drawbacks of batch processes such as large particle polydispersity and reduced batch-to-batch reproducibility are here overcome by using commercially available microfluidic reactors. Compared to the traditional batch...

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Authors: Garcia-Salinas, S., Himawan, E., Mendoza, G., Arruebo, M., Sebastian, V.
Format: article
Status:Versión aceptada para publicación
Publication Date:2018
Country:España
Institution:Universidad de Zaragoza
Repository:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:79329
Online Access:http://zaguan.unizar.es/record/79329
Access Level:Open access
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spelling Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow ReactorsGarcia-Salinas, S.Himawan, E.Mendoza, G.Arruebo, M.Sebastian, V.The large-scale continuous production of niosomes remains challenging. The inherent drawbacks of batch processes such as large particle polydispersity and reduced batch-to-batch reproducibility are here overcome by using commercially available microfluidic reactors. Compared to the traditional batch-based film hydration method, herein, we demonstrate that it is possible to carry out the homogeneous, large-scale (up to 120 mg/min) production of niosomes using two different synthesis techniques (the thin film hydration method and the emulsification technique). Niosomes particle size can be controlled depending on the need by varying the synthesis temperature. The high cytocompatibility of the resulting niosomes was also demonstrated in this work on three different somatic cell lines. For the first time, the structure of the niosome multilamellar shell was also elucidated using high-resolution transmission electron microscopy (HR-STEM) as well as their colloidal stability over time (6 weeks) under different storage conditions. The morphology of cryo-protected or as-made niosomes was also evaluated by HR-STEM after freeze-drying. Finally, the dual ability of those synthetic, nonionic, surfactant-based vesicles to carry both hydrophilic and hydrophobic molecules was also here demonstrated by using laser scanning confocal microscopy.2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://zaguan.unizar.es/record/79329reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/EC/FP7/614715info:eu-repo/semantics/openAccessoai:zaguan.unizar.es:793292026-05-29T13:59:51Z
dc.title.none.fl_str_mv Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
title Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
spellingShingle Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
Garcia-Salinas, S.
title_short Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
title_full Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
title_fullStr Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
title_full_unstemmed Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
title_sort Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
dc.creator.none.fl_str_mv Garcia-Salinas, S.
Himawan, E.
Mendoza, G.
Arruebo, M.
Sebastian, V.
author Garcia-Salinas, S.
author_facet Garcia-Salinas, S.
Himawan, E.
Mendoza, G.
Arruebo, M.
Sebastian, V.
author_role author
author2 Himawan, E.
Mendoza, G.
Arruebo, M.
Sebastian, V.
author2_role author
author
author
author
description The large-scale continuous production of niosomes remains challenging. The inherent drawbacks of batch processes such as large particle polydispersity and reduced batch-to-batch reproducibility are here overcome by using commercially available microfluidic reactors. Compared to the traditional batch-based film hydration method, herein, we demonstrate that it is possible to carry out the homogeneous, large-scale (up to 120 mg/min) production of niosomes using two different synthesis techniques (the thin film hydration method and the emulsification technique). Niosomes particle size can be controlled depending on the need by varying the synthesis temperature. The high cytocompatibility of the resulting niosomes was also demonstrated in this work on three different somatic cell lines. For the first time, the structure of the niosome multilamellar shell was also elucidated using high-resolution transmission electron microscopy (HR-STEM) as well as their colloidal stability over time (6 weeks) under different storage conditions. The morphology of cryo-protected or as-made niosomes was also evaluated by HR-STEM after freeze-drying. Finally, the dual ability of those synthetic, nonionic, surfactant-based vesicles to carry both hydrophilic and hydrophobic molecules was also here demonstrated by using laser scanning confocal microscopy.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/79329
url http://zaguan.unizar.es/record/79329
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/EC/FP7/614715
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
repository.name.fl_str_mv
repository.mail.fl_str_mv
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