How gelatin-based composite hydrogels influence the stability and release of reactive species generated in biopolymer solutions by non-thermal plasma treatment
In recent years, the applications of non-thermal plasma have been brought to light and have proven to be very promising in the biomedical field. Indeed, a plasma is composed of a large variety of reactive species, among them reactive oxygen and nitrogen species (RONS), that can act on a given sample...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/367555 |
| Acceso en línea: | https://hdl.handle.net/2117/367555 |
| Access Level: | acceso abierto |
| Palabra clave: | Biopolymers -- Biotechnology Plasma technology, Non-thermal Plasma Gelaton-based composite hydrogels Plasma Biopolímers -- Biotecnologia Hidrogel Biopolímers Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials |
| Sumario: | In recent years, the applications of non-thermal plasma have been brought to light and have proven to be very promising in the biomedical field. Indeed, a plasma is composed of a large variety of reactive species, among them reactive oxygen and nitrogen species (RONS), that can act on a given sample. One of the possibilities involves the delivery of plasma-derived reactive species for cancer treatments to obtain a less invasive procedure and avoid damaging the healthy cells. This study focusses focuses on how gelatin-based composite hydrogels influence the stability and release of reactive species generated in biopolymer solutions by non-thermal plasma treatment. The biopolymer solutions composed of Dermatan and Hyaluronic acid are treated to increase the amount of RONS compared to Plasma Treated Liquids (PTL). The use of gelatin hydrogels is justified to create easy-to-handle implants, while avoiding the biopolymer solution to be washed out by the body fluids after implantation. All components are from biological sources, biocompatible and biodegradable to avoid undesired interactions with the human body. The investigation of the biopolymer storage capacity will target H2O2, NO2 - and NO3 - , generated by a cold atmospheric plasma fed with a mixture of He + 0.3 % of synthetic air. In addition, to evaluate the behavior of the gelatin hydrogels, degradation tests were performed. Then the evaluation of RONS generation and a stability study were achieved. The results were compared according to the biopolymer used to store the plasma along with the detection methods employed. During the experiments, an increase of the RONS generated in Dermatan and Hyaluronic acid solution was observed, compared to the ones generated in PBS. We could advance a potential continued release of the RONS from the hydrogels after 45 minutes in physiological conditions. |
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