Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread

The contamination of adenovirus (Ad) stocks with adeno-associated viruses (AAV) is usually unnoticed, and it has been associated with lower Ad yields upon large-scale production. During Ad propagation, AAV contamination needs to be detected routinely by polymerase chain reaction without symptomatic...

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Detalles Bibliográficos
Autores: Laborda, Eduardo, Puig Saus, Cristina, Cascallò, Manel, Chillón, Miguel, Alemany Bonastre, Ramon
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/123876
Acceso en línea:https://hdl.handle.net/2445/123876
Access Level:acceso abierto
Palabra clave:Adenovirus
Malalties víriques
Adenoviruses
Virus diseases
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spelling Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus SpreadLaborda, EduardoPuig Saus, CristinaCascallò, ManelChillón, MiguelAlemany Bonastre, RamonAdenovirusMalalties víriquesAdenovirusesVirus diseasesThe contamination of adenovirus (Ad) stocks with adeno-associated viruses (AAV) is usually unnoticed, and it has been associated with lower Ad yields upon large-scale production. During Ad propagation, AAV contamination needs to be detected routinely by polymerase chain reaction without symptomatic suspicion. In this study, we describe that the coinfection of either Ad wild type 5 or oncolytic Ad with AAV results in a large-plaque phenotype associated with an accelerated release of Ad from coinfected cells. This accelerated release was accompanied with the expected decrease in Ad yields in two out of three cell lines tested. Despite this lower Ad yield, coinfection with AAV accelerated cell death and enhanced the cytotoxicity mediated by Ad propagation. Intratumoral coinjection of Ad and AAV in two xenograft tumor models improved antitumor activity and mouse survival. Therefore, we conclude that accidental or intentional AAV coinfection has important implications for Ad-mediated virotherapy.Mary Ann Liebert2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/123876Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1089/hgtb.2013.124Human Gene Therapy Methods, 2013, vol. 24, núm. 6, p. 372-380http://dx.doi.org/10.1089/hgtb.2013.124(c) Mary Ann Liebert, 2013info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1238762026-05-27T06:46:51Z
dc.title.none.fl_str_mv Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
title Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
spellingShingle Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
Laborda, Eduardo
Adenovirus
Malalties víriques
Adenoviruses
Virus diseases
title_short Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
title_full Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
title_fullStr Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
title_full_unstemmed Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
title_sort Adeno-associated Virus Enhances Wild-type And Oncolytic Adenovirus Spread
dc.creator.none.fl_str_mv Laborda, Eduardo
Puig Saus, Cristina
Cascallò, Manel
Chillón, Miguel
Alemany Bonastre, Ramon
author Laborda, Eduardo
author_facet Laborda, Eduardo
Puig Saus, Cristina
Cascallò, Manel
Chillón, Miguel
Alemany Bonastre, Ramon
author_role author
author2 Puig Saus, Cristina
Cascallò, Manel
Chillón, Miguel
Alemany Bonastre, Ramon
author2_role author
author
author
author
dc.subject.none.fl_str_mv Adenovirus
Malalties víriques
Adenoviruses
Virus diseases
topic Adenovirus
Malalties víriques
Adenoviruses
Virus diseases
description The contamination of adenovirus (Ad) stocks with adeno-associated viruses (AAV) is usually unnoticed, and it has been associated with lower Ad yields upon large-scale production. During Ad propagation, AAV contamination needs to be detected routinely by polymerase chain reaction without symptomatic suspicion. In this study, we describe that the coinfection of either Ad wild type 5 or oncolytic Ad with AAV results in a large-plaque phenotype associated with an accelerated release of Ad from coinfected cells. This accelerated release was accompanied with the expected decrease in Ad yields in two out of three cell lines tested. Despite this lower Ad yield, coinfection with AAV accelerated cell death and enhanced the cytotoxicity mediated by Ad propagation. Intratumoral coinjection of Ad and AAV in two xenograft tumor models improved antitumor activity and mouse survival. Therefore, we conclude that accidental or intentional AAV coinfection has important implications for Ad-mediated virotherapy.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/123876
url https://hdl.handle.net/2445/123876
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1089/hgtb.2013.124
Human Gene Therapy Methods, 2013, vol. 24, núm. 6, p. 372-380
http://dx.doi.org/10.1089/hgtb.2013.124
dc.rights.none.fl_str_mv (c) Mary Ann Liebert, 2013
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Mary Ann Liebert, 2013
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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