Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte...

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Detalhes bibliográficos
Autores: Silva, Sonia, Altmannova, Veronika, Luke Glaser, Sarah, Henriksen, Peter, Gallina, Irene, Yang, Xuejiao, Choudhary, Chunaram, Luke, Brian, Krejci, Lumir, Lisby, Michael
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/81686
Acesso em linha:https://hdl.handle.net/11441/81686
https://doi.org/10.1101/gad.276204.115
Access Level:acceso abierto
Palavra-chave:Homologous recombination
Telomere maintenance
Genome integrity
DNA repair
Mph1
Mte1
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repository_id_str
spelling Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenanceSilva, SoniaAltmannova, VeronikaLuke Glaser, SarahHenriksen, PeterGallina, IreneYang, XuejiaoChoudhary, ChunaramLuke, BrianKrejci, LumirLisby, MichaelHomologous recombinationTelomere maintenanceGenome integrityDNA repairMph1Mte1Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize atDNAdamage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.Cold Spring Harbor Laboratory Press2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/81686https://doi.org/10.1101/gad.276204.115reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésGenes and Development, 30, 700-717.https://doi.org/10.1101/gad.276204.115info:eu-repo/semantics/openAccessoai:idus.us.es:11441/816862026-06-17T12:51:07Z
dc.title.none.fl_str_mv Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
title Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
spellingShingle Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
Silva, Sonia
Homologous recombination
Telomere maintenance
Genome integrity
DNA repair
Mph1
Mte1
title_short Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
title_full Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
title_fullStr Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
title_full_unstemmed Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
title_sort Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
dc.creator.none.fl_str_mv Silva, Sonia
Altmannova, Veronika
Luke Glaser, Sarah
Henriksen, Peter
Gallina, Irene
Yang, Xuejiao
Choudhary, Chunaram
Luke, Brian
Krejci, Lumir
Lisby, Michael
author Silva, Sonia
author_facet Silva, Sonia
Altmannova, Veronika
Luke Glaser, Sarah
Henriksen, Peter
Gallina, Irene
Yang, Xuejiao
Choudhary, Chunaram
Luke, Brian
Krejci, Lumir
Lisby, Michael
author_role author
author2 Altmannova, Veronika
Luke Glaser, Sarah
Henriksen, Peter
Gallina, Irene
Yang, Xuejiao
Choudhary, Chunaram
Luke, Brian
Krejci, Lumir
Lisby, Michael
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Homologous recombination
Telomere maintenance
Genome integrity
DNA repair
Mph1
Mte1
topic Homologous recombination
Telomere maintenance
Genome integrity
DNA repair
Mph1
Mte1
description Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize atDNAdamage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/81686
https://doi.org/10.1101/gad.276204.115
url https://hdl.handle.net/11441/81686
https://doi.org/10.1101/gad.276204.115
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Genes and Development, 30, 700-717.
https://doi.org/10.1101/gad.276204.115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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