Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)

[Background] Marine species have colonized extreme environments during evolution such as freshwater habitats. The amphidromous teleost fish, Galaxias maculatus is found mainly migrating between estuaries and rivers, but some landlocked populations have been described in lakes formed during the last...

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Autores: Ruiz-Jarabo, Ignacio, González-Wevar, Claudio A., Oyarzún, Ricardo, Fuentes, Juan, Poulin, Elie, Bertrán, C., Vargas-Chacoff, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/343963
Acceso en línea:http://hdl.handle.net/10261/343963
Access Level:acceso abierto
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spelling Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)Ruiz-Jarabo, IgnacioGonzález-Wevar, Claudio A.Oyarzún, RicardoFuentes, JuanPoulin, ElieBertrán, C.Vargas-Chacoff, Luis[Background] Marine species have colonized extreme environments during evolution such as freshwater habitats. The amphidromous teleost fish, Galaxias maculatus is found mainly migrating between estuaries and rivers, but some landlocked populations have been described in lakes formed during the last deglaciation process in the Andes. In the present study we use mtDNA sequences to reconstruct the historical scenario of colonization of such a lake and evaluated the osmoregulatory shift associated to changes in habitat and life cycle between amphidromous and landlocked populations.[Results] Standard diversity indices including the average number of nucleotide differences (Π) and the haplotype diversity index (H) indicated that both populations were, as expected, genetically distinctive, being the landlocked population less diverse than the diadromous one. Similarly, pairwise GST and NST comparison detected statistically significant differences between both populations, while genealogy of haplotypes evidenced a recent founder effect from the diadromous stock, followed by an expansion process in the lake. To test for physiological differences, individuals of both populations were challenged with a range of salinities from 0 to 30 ppt for 8 days following a period of progressive acclimation. The results showed that the landlocked population had a surprisingly wider tolerance to salinity, as landlocked fish survival was 100% from 0 to 20 ppt, whereas diadromous fish survival was 100% only from 10 to 15 ppt. The activity of ATPase enzymes, including Na+/K+-ATPase (NKA), and H+-ATPase (HA) was measured in gills and intestine. Activity differences were detected between the populations at the lowest salinities, including differences in ATPases other than NKA and HA. Population differences in mortality are not reflected in enzyme activity differences, suggesting divergence in other processes.[Conclusions] These results clearly demonstrate the striking adaptive changes of G. maculatus osmoregulatory system, especially at hyposmotic environments, associated to a drastic shift in habitat and life cycle at a scale of a few thousand years.This work was partially supported by a Ph. D. scholarship from the University of Cadiz (UCA 2009-074-FPI) to I. R-J. It was also partly supported by the grants Fondecyt 1151375, 1160877 and Fondap-IDEAL 15150003 (Chile) to L.V-C, the post doctorate fellowship (Fondecyt 3120075) and Initiation Project 11140087 to C.A.G-W. We also thank Instituto de Ecología y Biodiversidad (IEB) projects P05-002 ICM and PFB 023 to E.P. and C.A. G-WPeer reviewedPublic Library of ScienceUniversidad de CádizFondo Nacional de Desarrollo Científico y Tecnológico (Chile)Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (Chile)Centro de Investigación Dinámica de Ecosistemas Marinos de Altas Latitudes (Chile)Instituto de Ecología y Biodiversidad (Chile)202420242016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/343963reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1371/journal.pone.0154766Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3439632026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
title Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
spellingShingle Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
Ruiz-Jarabo, Ignacio
title_short Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
title_full Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
title_fullStr Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
title_full_unstemmed Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
title_sort Isolation Driven Divergence in Osmoregulation in Galaxias maculatus (Jenyns, 1848) (Actinopterygii: Osmeriformes)
dc.creator.none.fl_str_mv Ruiz-Jarabo, Ignacio
González-Wevar, Claudio A.
Oyarzún, Ricardo
Fuentes, Juan
Poulin, Elie
Bertrán, C.
Vargas-Chacoff, Luis
author Ruiz-Jarabo, Ignacio
author_facet Ruiz-Jarabo, Ignacio
González-Wevar, Claudio A.
Oyarzún, Ricardo
Fuentes, Juan
Poulin, Elie
Bertrán, C.
Vargas-Chacoff, Luis
author_role author
author2 González-Wevar, Claudio A.
Oyarzún, Ricardo
Fuentes, Juan
Poulin, Elie
Bertrán, C.
Vargas-Chacoff, Luis
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cádiz
Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (Chile)
Centro de Investigación Dinámica de Ecosistemas Marinos de Altas Latitudes (Chile)
Instituto de Ecología y Biodiversidad (Chile)
description [Background] Marine species have colonized extreme environments during evolution such as freshwater habitats. The amphidromous teleost fish, Galaxias maculatus is found mainly migrating between estuaries and rivers, but some landlocked populations have been described in lakes formed during the last deglaciation process in the Andes. In the present study we use mtDNA sequences to reconstruct the historical scenario of colonization of such a lake and evaluated the osmoregulatory shift associated to changes in habitat and life cycle between amphidromous and landlocked populations.
publishDate 2016
dc.date.none.fl_str_mv 2016
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/343963
url http://hdl.handle.net/10261/343963
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1371/journal.pone.0154766
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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