Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis

Oenococcus oeni is usually the main lactic acid bacteria (LAB) responsible for conducting malolactic fermentation (MLF) in wines. Pulsed Field Gel Electrophoresis (PFGE) is one of the most common methods used to identify different genotypes among the wine LAB populations. Although PFGE is a powerful...

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Detalhes bibliográficos
Autores: González-Arenzana, L. [0000-0001-5181-3173], Santamaría, P., López, R., López-Alfaro, I. [0000-0002-6438-423X]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc69dcb750603269e822a6
Acesso em linha:https://investigacion.unirioja.es/documentos/5bbc69dcb750603269e822a6
Access Level:acceso abierto
Palavra-chave:MLST
Oenococcus oeni
PFGE
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spelling Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysisGonzález-Arenzana, L. [0000-0001-5181-3173]Santamaría, P.López, R.López-Alfaro, I. [0000-0002-6438-423X]MLSTOenococcus oeniPFGEOenococcus oeni is usually the main lactic acid bacteria (LAB) responsible for conducting malolactic fermentation (MLF) in wines. Pulsed Field Gel Electrophoresis (PFGE) is one of the most common methods used to identify different genotypes among the wine LAB populations. Although PFGE is a powerful typing tool, it is time-consuming and its results are not easily exchangeable between laboratories so typing methods such as Multilocus Sequence Typing (MLST) have been developed. In this study, thirty O. oeni isolates from Rioja Tempranillo wines were characterized performing SfiI and ApaI PFGE and MLST with eight housekeeping genes. Using the latter technique, six new alleles have been described for five genes. PFGE was slightly more efficient than MLST because of the number of genotypes and of the index of diversity (ID) that each technique discriminated. This has been the first time that PFGE and MLST results have been combined to shape a unique dendrogram. Thus, the combination of results from both typing methods allowed the discrimination of twenty-two PFGE-ST genotypes showing the highest ID of these research (0.947). According to these results, the future application of the combination of PFGE and MLST results could be successful for reliable O. oeni strain typification. © 2013 Elsevier Ltd. All rights reserved.2014info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc69dcb750603269e822a6reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/J.FM.2013.07.014info:eu-repo/semantics/altIdentifier/wos/WOS:000328660400036info:eu-repo/semantics/altIdentifier/pissn/0740-0020Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis, 2014, vol. 38, pág. 295-302info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc69dcb750603269e822a62026-06-14T12:47:17Z
dc.title.none.fl_str_mv Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
title Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
spellingShingle Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
González-Arenzana, L. [0000-0001-5181-3173]
MLST
Oenococcus oeni
PFGE
title_short Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
title_full Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
title_fullStr Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
title_full_unstemmed Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
title_sort Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis
dc.creator.none.fl_str_mv González-Arenzana, L. [0000-0001-5181-3173]
Santamaría, P.
López, R.
López-Alfaro, I. [0000-0002-6438-423X]
author González-Arenzana, L. [0000-0001-5181-3173]
author_facet González-Arenzana, L. [0000-0001-5181-3173]
Santamaría, P.
López, R.
López-Alfaro, I. [0000-0002-6438-423X]
author_role author
author2 Santamaría, P.
López, R.
López-Alfaro, I. [0000-0002-6438-423X]
author2_role author
author
author
dc.subject.none.fl_str_mv MLST
Oenococcus oeni
PFGE
topic MLST
Oenococcus oeni
PFGE
description Oenococcus oeni is usually the main lactic acid bacteria (LAB) responsible for conducting malolactic fermentation (MLF) in wines. Pulsed Field Gel Electrophoresis (PFGE) is one of the most common methods used to identify different genotypes among the wine LAB populations. Although PFGE is a powerful typing tool, it is time-consuming and its results are not easily exchangeable between laboratories so typing methods such as Multilocus Sequence Typing (MLST) have been developed. In this study, thirty O. oeni isolates from Rioja Tempranillo wines were characterized performing SfiI and ApaI PFGE and MLST with eight housekeeping genes. Using the latter technique, six new alleles have been described for five genes. PFGE was slightly more efficient than MLST because of the number of genotypes and of the index of diversity (ID) that each technique discriminated. This has been the first time that PFGE and MLST results have been combined to shape a unique dendrogram. Thus, the combination of results from both typing methods allowed the discrimination of twenty-two PFGE-ST genotypes showing the highest ID of these research (0.947). According to these results, the future application of the combination of PFGE and MLST results could be successful for reliable O. oeni strain typification. © 2013 Elsevier Ltd. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc69dcb750603269e822a6
url https://investigacion.unirioja.es/documentos/5bbc69dcb750603269e822a6
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/J.FM.2013.07.014
info:eu-repo/semantics/altIdentifier/wos/WOS:000328660400036
info:eu-repo/semantics/altIdentifier/pissn/0740-0020
Oenococcus oeni strain typification by combination of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis analysis, 2014, vol. 38, pág. 295-302
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
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