Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.

Five bacterial (facultatively) anaerobic strains, namely Buttiauxella sp. MASE-IM-9, Clostridium sp. MASE-IM-4, Halanaerobium sp. MASE-BB-1, Trichococcus sp. MASE-IM-5, and Yersinia intermedia MASE-LG-1 isolated from different extreme natural environments were subjected to Mars relevant environmenta...

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Autores: Beblo Vranesevic, K., Bohmeier, M., Schleumer, S., Rabbow, Elke, Perras, A. K., Moissl-Eichinger, Christine, Schwendner, P., Cockell, Charles S., Vannier, P., Marteinsson, V. T., Monaghan, E. P., Riedo, A., Ehrenfreund, P., García Descalzo, L., Gómez, Felipe, Malki, M., Amils Pibernat, R., Gaboyer, F., Hickman-Lewis, K., Westall, Frances, Cabezas, Patricia, Walter, N., Rettberg, P.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Instituto Nacional de Técnica Aeroespacial (INTA)
Repositorio:DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
OAI Identifier:oai:digital.inta.es:20.500.12666/208
Acceso en línea:https://www.caister.com/cimb/abstracts/v38/103.html
http://hdl.handle.net/20.500.12666/208
Access Level:acceso abierto
Palabra clave:Martian conditions
Mars
Anaerobic Bacterial
Simulated
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spelling Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.Beblo Vranesevic, K.Bohmeier, M. Schleumer, S.Rabbow, ElkePerras, A. K.Moissl-Eichinger, ChristineSchwendner, P.Cockell, Charles S.Vannier, P.Marteinsson, V. T.Monaghan, E. P.Riedo, A.Ehrenfreund, P.García Descalzo, L.Gómez, FelipeMalki, M.Amils Pibernat, R.Gaboyer, F.Hickman-Lewis, K.Westall, FrancesCabezas, PatriciaWalter, N.Rettberg, P.Martian conditionsMarsAnaerobic BacterialSimulatedFive bacterial (facultatively) anaerobic strains, namely Buttiauxella sp. MASE-IM-9, Clostridium sp. MASE-IM-4, Halanaerobium sp. MASE-BB-1, Trichococcus sp. MASE-IM-5, and Yersinia intermedia MASE-LG-1 isolated from different extreme natural environments were subjected to Mars relevant environmental stress factors in the laboratory under controlled conditions. These stress factors encompassed low water activity, oxidizing compounds, and ionizing radiation. Stress tests were performed under permanently anoxic conditions. The survival rate after addition of sodium perchlorate (Na-perchlorate) was found to be species-specific. The inter-comparison of the five microorganisms revealed that Clostridium sp. MASE-IM-4 was the most sensitive strain (D-10-value (15 min, NaClO4) = 0.6 M). The most tolerant microorganism was Trichococcus sp. MASE-IM-5 with a calculated D-10-value (15 min, NaClO4) of 1.9 M. Cultivation in the presence of Na-perchlorate in Martian relevant concentrations up to 1 wt% led to the observation of chains of cells in all strains. Exposure to Na-perchlorate led to a lowering of the survival rate after desiccation. Consecutive exposure to desiccating conditions and ionizing radiation led to additive effects. Moreover, in a desiccated state, an enhanced radiation tolerance could be observed for the strains Clostridium sp. MASE-IM-4 and Trichococcus sp. MASE-IM-5. These data show that anaerobic micro-organisms from Mars analogue environments can resist a variety of Martian-simulated stresses either individually or in combination. However, responses were species-specific and some Mars-simulated extremes killed certain organisms. Thus, although Martian stresses would be expected to act differentially on microorganisms, none of the expected extremes tested here and found on Mars prevent the growth of anaerobic microorganisms.Peer reviewMultidisciplinary Digital Publishing Institute (MDPI)Rettberg, P. [0000-0003-4439-2395]García Descalzo, L. [0000-0002-0083-6786]Cabezas, P. [0000-0002-6336-4093]Marteinsson, V. [0000-0001-8340-821X]202120212020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501application/pdfhttps://www.caister.com/cimb/abstracts/v38/103.htmlhttp://hdl.handle.net/20.500.12666/208reponame:DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacialinstname:Instituto Nacional de Técnica Aeroespacial (INTA)InglésAttribution-NonCommercial-NoDerivatives 4.0 InternationalCaister Academic Presshttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:digital.inta.es:20.500.12666/2082026-06-23T12:46:37Z
dc.title.none.fl_str_mv Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
title Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
spellingShingle Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
Beblo Vranesevic, K.
Martian conditions
Mars
Anaerobic Bacterial
Simulated
title_short Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
title_full Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
title_fullStr Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
title_full_unstemmed Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
title_sort Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites.
dc.creator.none.fl_str_mv Beblo Vranesevic, K.
Bohmeier, M.
Schleumer, S.
Rabbow, Elke
Perras, A. K.
Moissl-Eichinger, Christine
Schwendner, P.
Cockell, Charles S.
Vannier, P.
Marteinsson, V. T.
Monaghan, E. P.
Riedo, A.
Ehrenfreund, P.
García Descalzo, L.
Gómez, Felipe
Malki, M.
Amils Pibernat, R.
Gaboyer, F.
Hickman-Lewis, K.
Westall, Frances
Cabezas, Patricia
Walter, N.
Rettberg, P.
author Beblo Vranesevic, K.
author_facet Beblo Vranesevic, K.
Bohmeier, M.
Schleumer, S.
Rabbow, Elke
Perras, A. K.
Moissl-Eichinger, Christine
Schwendner, P.
Cockell, Charles S.
Vannier, P.
Marteinsson, V. T.
Monaghan, E. P.
Riedo, A.
Ehrenfreund, P.
García Descalzo, L.
Gómez, Felipe
Malki, M.
Amils Pibernat, R.
Gaboyer, F.
Hickman-Lewis, K.
Westall, Frances
Cabezas, Patricia
Walter, N.
Rettberg, P.
author_role author
author2 Bohmeier, M.
Schleumer, S.
Rabbow, Elke
Perras, A. K.
Moissl-Eichinger, Christine
Schwendner, P.
Cockell, Charles S.
Vannier, P.
Marteinsson, V. T.
Monaghan, E. P.
Riedo, A.
Ehrenfreund, P.
García Descalzo, L.
Gómez, Felipe
Malki, M.
Amils Pibernat, R.
Gaboyer, F.
Hickman-Lewis, K.
Westall, Frances
Cabezas, Patricia
Walter, N.
Rettberg, P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Rettberg, P. [0000-0003-4439-2395]
García Descalzo, L. [0000-0002-0083-6786]
Cabezas, P. [0000-0002-6336-4093]
Marteinsson, V. [0000-0001-8340-821X]
dc.subject.none.fl_str_mv Martian conditions
Mars
Anaerobic Bacterial
Simulated
topic Martian conditions
Mars
Anaerobic Bacterial
Simulated
description Five bacterial (facultatively) anaerobic strains, namely Buttiauxella sp. MASE-IM-9, Clostridium sp. MASE-IM-4, Halanaerobium sp. MASE-BB-1, Trichococcus sp. MASE-IM-5, and Yersinia intermedia MASE-LG-1 isolated from different extreme natural environments were subjected to Mars relevant environmental stress factors in the laboratory under controlled conditions. These stress factors encompassed low water activity, oxidizing compounds, and ionizing radiation. Stress tests were performed under permanently anoxic conditions. The survival rate after addition of sodium perchlorate (Na-perchlorate) was found to be species-specific. The inter-comparison of the five microorganisms revealed that Clostridium sp. MASE-IM-4 was the most sensitive strain (D-10-value (15 min, NaClO4) = 0.6 M). The most tolerant microorganism was Trichococcus sp. MASE-IM-5 with a calculated D-10-value (15 min, NaClO4) of 1.9 M. Cultivation in the presence of Na-perchlorate in Martian relevant concentrations up to 1 wt% led to the observation of chains of cells in all strains. Exposure to Na-perchlorate led to a lowering of the survival rate after desiccation. Consecutive exposure to desiccating conditions and ionizing radiation led to additive effects. Moreover, in a desiccated state, an enhanced radiation tolerance could be observed for the strains Clostridium sp. MASE-IM-4 and Trichococcus sp. MASE-IM-5. These data show that anaerobic micro-organisms from Mars analogue environments can resist a variety of Martian-simulated stresses either individually or in combination. However, responses were species-specific and some Mars-simulated extremes killed certain organisms. Thus, although Martian stresses would be expected to act differentially on microorganisms, none of the expected extremes tested here and found on Mars prevent the growth of anaerobic microorganisms.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.caister.com/cimb/abstracts/v38/103.html
http://hdl.handle.net/20.500.12666/208
url https://www.caister.com/cimb/abstracts/v38/103.html
http://hdl.handle.net/20.500.12666/208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
Caister Academic Press
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
Caister Academic Press
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
instname:Instituto Nacional de Técnica Aeroespacial (INTA)
instname_str Instituto Nacional de Técnica Aeroespacial (INTA)
reponame_str DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
collection DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
repository.name.fl_str_mv
repository.mail.fl_str_mv
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