Detection and quantification of zearalenone and its modified forms in enzymatically treated oat and wheat flour
An analytical method for the analysis of the mycotoxin zearalenone (ZEN) and its modifed forms was developed. Sample preparation was performed based on a modifed QuEChERS method combined with liquid chromatography coupled to a triple quadrupole mass spectrometry detection. The method was tested for...
| Autores: | , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/463077 |
| Acesso em linha: | https://doi.org/10.1007/s13197-023-05683-6 https://hdl.handle.net/10459.1/463077 |
| Access Level: | acceso abierto |
| Palavra-chave: | Amiloïdosi MS/MS QuEChERS Zearalenol |
| Resumo: | An analytical method for the analysis of the mycotoxin zearalenone (ZEN) and its modifed forms was developed. Sample preparation was performed based on a modifed QuEChERS method combined with liquid chromatography coupled to a triple quadrupole mass spectrometry detection. The method was tested for linearity, precision, limits of detection and quantifcation and recoveries. The evaluation of the above-mentioned parameters was performed on oat four. The method was applied to oat and wheat fours that were submitted to an amylolytic treatment (α-amylase and amyloglucosidase), similar to the one used in the cereal-based baby food production process. A decrease in β-zearalenol (β-ZEL) and β-ZEL-14-sulfate of approximately 40% after 90 min incubation was observed, the other analytes did not show any signifcant changes. To our knowledge, this is the frst method that approaches the identifcation and assessment of ZEN-sulfate derivates in a cereal matrix. |
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