Effect of temperature and flow rate on the cell-free area in the microfluidic channel

Blood cell manipulation in microdevices is an interesting task for the separation of particles, by their size, density, or to remove them from the buffer, in which they are suspended, for further analysis, and more. This study highlights the cell-free area (CFA) widening based on experimental result...

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Detalhes bibliográficos
Autores: Rodríguez-Villarreal, Ivón, Carmona Flores, Manuel, Colomer i Farrarons, Jordi
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176380
Acesso em linha:https://hdl.handle.net/2445/176380
Access Level:acceso abierto
Palavra-chave:Microfluídica
Plasma sanguini
Circulació sanguínia
Microfluidics
Blood plasma
Circulation of the blood
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spelling Effect of temperature and flow rate on the cell-free area in the microfluidic channelRodríguez-Villarreal, IvónCarmona Flores, ManuelColomer i Farrarons, JordiMicrofluídicaPlasma sanguiniCirculació sanguíniaMicrofluidicsBlood plasmaCirculation of the bloodBlood cell manipulation in microdevices is an interesting task for the separation of particles, by their size, density, or to remove them from the buffer, in which they are suspended, for further analysis, and more. This study highlights the cell-free area (CFA) widening based on experimental results of red blood cell (RBC) flow, suspended in a microfluidic device, while temperature and flow rate incrementally modify RBC response within the microflow. Studies of human red blood cell flow, at a concentration of 20%, suspended in its autologous plasma and phosphate-buffered saline (PBS) buffer, were carried out at a wide flow rate, varying between 10 and 230 µL/min and a temperature range of 23 ◦C to 50 ◦C. The plotted measures show an increment in a CFA near the channel wall due to cell flow inertia after a constricted channel, which becomes more significant as temperature and flow rate increase. The temperature increment widened the CFA up to three times. In comparison, flow rate increment increased the CFA up to 20 times in PBS and 11 times in plasma.MDPI2021202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/176380Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/membranes11020109Membranes, 2021, vol. 11, num. 2, p. 109-122https://doi.org/10.3390/membranes11020109cc-by (c) Rodríguez-Villarreal, Ivón et al., 2021http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1763802026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effect of temperature and flow rate on the cell-free area in the microfluidic channel
title Effect of temperature and flow rate on the cell-free area in the microfluidic channel
spellingShingle Effect of temperature and flow rate on the cell-free area in the microfluidic channel
Rodríguez-Villarreal, Ivón
Microfluídica
Plasma sanguini
Circulació sanguínia
Microfluidics
Blood plasma
Circulation of the blood
title_short Effect of temperature and flow rate on the cell-free area in the microfluidic channel
title_full Effect of temperature and flow rate on the cell-free area in the microfluidic channel
title_fullStr Effect of temperature and flow rate on the cell-free area in the microfluidic channel
title_full_unstemmed Effect of temperature and flow rate on the cell-free area in the microfluidic channel
title_sort Effect of temperature and flow rate on the cell-free area in the microfluidic channel
dc.creator.none.fl_str_mv Rodríguez-Villarreal, Ivón
Carmona Flores, Manuel
Colomer i Farrarons, Jordi
author Rodríguez-Villarreal, Ivón
author_facet Rodríguez-Villarreal, Ivón
Carmona Flores, Manuel
Colomer i Farrarons, Jordi
author_role author
author2 Carmona Flores, Manuel
Colomer i Farrarons, Jordi
author2_role author
author
dc.subject.none.fl_str_mv Microfluídica
Plasma sanguini
Circulació sanguínia
Microfluidics
Blood plasma
Circulation of the blood
topic Microfluídica
Plasma sanguini
Circulació sanguínia
Microfluidics
Blood plasma
Circulation of the blood
description Blood cell manipulation in microdevices is an interesting task for the separation of particles, by their size, density, or to remove them from the buffer, in which they are suspended, for further analysis, and more. This study highlights the cell-free area (CFA) widening based on experimental results of red blood cell (RBC) flow, suspended in a microfluidic device, while temperature and flow rate incrementally modify RBC response within the microflow. Studies of human red blood cell flow, at a concentration of 20%, suspended in its autologous plasma and phosphate-buffered saline (PBS) buffer, were carried out at a wide flow rate, varying between 10 and 230 µL/min and a temperature range of 23 ◦C to 50 ◦C. The plotted measures show an increment in a CFA near the channel wall due to cell flow inertia after a constricted channel, which becomes more significant as temperature and flow rate increase. The temperature increment widened the CFA up to three times. In comparison, flow rate increment increased the CFA up to 20 times in PBS and 11 times in plasma.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176380
url https://hdl.handle.net/2445/176380
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/membranes11020109
Membranes, 2021, vol. 11, num. 2, p. 109-122
https://doi.org/10.3390/membranes11020109
dc.rights.none.fl_str_mv cc-by (c) Rodríguez-Villarreal, Ivón et al., 2021
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Rodríguez-Villarreal, Ivón et al., 2021
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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