Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif
21 p.- 7 fig.- 4 tab.-2 fig. supl.-1 tab. supl.
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/52343 |
| Acceso en línea: | http://hdl.handle.net/10261/52343 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacteriophage Biophysics Circular dichroism Computer modeling Ultracentrifugation CW-7 motif Cpl-7 endolysin structure SAXS Streptococcus pneumoniae Cell wall hydrolases |
| id |
ES_a94d5dc286ba3e06c4ee5b9089efdda7 |
|---|---|
| oai_identifier_str |
oai:digital.csic.es:10261/52343 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding MotifBustamante, NoemíCampillo, Nuria E.García, ErnestoGallego-Páramo, CristinaPera, BenetDiakun, Gregory P.Sáiz, José LuisGarcía González, PedroDíaz, José FernandoMenéndez, MargaritaBacteriophageBiophysicsCircular dichroismComputer modelingUltracentrifugationCW-7 motifCpl-7 endolysin structureSAXSStreptococcus pneumoniaeCell wall hydrolases21 p.- 7 fig.- 4 tab.-2 fig. supl.-1 tab. supl.Bacteriophage endolysins include a group of new antibacterials reluctant to development of resistance. We present here the first structural study of the Cpl-7 endolysin, encoded by pneumococcal bacteriophage Cp-7. It contains an N-terminal catalytic module (CM) belonging to the GH25 family of glycosyl hydrolases and a C-terminal region encompassing three identical repeats of 42 amino acids (CW_7 repeats). These repeats are unrelated to choline-targeting motifs present in other cell wall hydrolases produced by Streptococcus pneumoniae and its bacteriophages, and are responsible for the protein attachment to the cell wall. By combining different biophysical techniques and molecular modeling, a three-dimensional model of the overall protein structure is proposed, consistent with circular dichroism and sequence-based secondary structure prediction, small angle x-ray scattering data, and Cpl-7 hydrodynamic behavior. Cpl-7 is an ∼115-Å long molecule with two well differentiated regions, corresponding to the CM and the cell wall binding region (CWBR), arranged in a lateral disposition. The CM displays the (βα)5β3 barrel topology characteristic of the GH25 family, and the impact of sequence differences with the CM of the Cpl-1 lysozyme in substrate binding is discussed. The CWBR is organized in three tandemly assembled three-helical bundles whose dispositions remind us of a super-helical structure. Its approximate dimensions are 60 × 20 × 20 Å and presents a concave face that might constitute the functional region involved in bacterial surface recognition. The distribution of CW_7 repeats in the sequences deposited in the Entrez Database have been examined, and the results drastically expanded the antimicrobial potential of the Cpl-7 endolysinThis work was supported by Grants BFU2006-10288, SAF2009-10824, BIO2007-61336, and BFU2009-10052 from Ministerio de Ciencia e Innovación, Grant BIPPED-CM from the Comunidad de Madrid, the CIBER de Enfermedades Respiratorias (CIBERES), an initiative of the ISCIII, Glycodynamics Network Grant FP6-UE MCTN-CT-2005-019561, and European Community beam time Proposal 48086Peer reviewedAmerican Society for Biochemistry and Molecular Biology2010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/52343reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1074/jbc.M110.154559info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/523432026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| title |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| spellingShingle |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif Bustamante, Noemí Bacteriophage Biophysics Circular dichroism Computer modeling Ultracentrifugation CW-7 motif Cpl-7 endolysin structure SAXS Streptococcus pneumoniae Cell wall hydrolases |
| title_short |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| title_full |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| title_fullStr |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| title_full_unstemmed |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| title_sort |
Cpl-7, a Lysozyme Encoded by a Pneumococcal Bacteriophage with a Novel Cell Wall-Binding Motif |
| dc.creator.none.fl_str_mv |
Bustamante, Noemí Campillo, Nuria E. García, Ernesto Gallego-Páramo, Cristina Pera, Benet Diakun, Gregory P. Sáiz, José Luis García González, Pedro Díaz, José Fernando Menéndez, Margarita |
| author |
Bustamante, Noemí |
| author_facet |
Bustamante, Noemí Campillo, Nuria E. García, Ernesto Gallego-Páramo, Cristina Pera, Benet Diakun, Gregory P. Sáiz, José Luis García González, Pedro Díaz, José Fernando Menéndez, Margarita |
| author_role |
author |
| author2 |
Campillo, Nuria E. García, Ernesto Gallego-Páramo, Cristina Pera, Benet Diakun, Gregory P. Sáiz, José Luis García González, Pedro Díaz, José Fernando Menéndez, Margarita |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bacteriophage Biophysics Circular dichroism Computer modeling Ultracentrifugation CW-7 motif Cpl-7 endolysin structure SAXS Streptococcus pneumoniae Cell wall hydrolases |
| topic |
Bacteriophage Biophysics Circular dichroism Computer modeling Ultracentrifugation CW-7 motif Cpl-7 endolysin structure SAXS Streptococcus pneumoniae Cell wall hydrolases |
| description |
21 p.- 7 fig.- 4 tab.-2 fig. supl.-1 tab. supl. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/52343 |
| url |
http://hdl.handle.net/10261/52343 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://dx.doi.org/10.1074/jbc.M110.154559 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
American Society for Biochemistry and Molecular Biology |
| publisher.none.fl_str_mv |
American Society for Biochemistry and Molecular Biology |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869416009192964096 |
| score |
15,812429 |