Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells

During insect oogenesis, the follicular epithelium undergoes both cell proliferation and apoptosis, thus modulating ovarian follicle growth. The Hippo pathway is key in these processes, and has been thoroughly studied in the meroistic ovaries of Drosophila melanogaster. However, nothing is known abo...

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Detalles Bibliográficos
Autores: Irles, Paula, Piulachs, Maria-Dolors
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/115747
Acceso en línea:http://hdl.handle.net/10261/115747
Access Level:acceso abierto
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spelling Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cellsIrles, PaulaPiulachs, Maria-DolorsDuring insect oogenesis, the follicular epithelium undergoes both cell proliferation and apoptosis, thus modulating ovarian follicle growth. The Hippo pathway is key in these processes, and has been thoroughly studied in the meroistic ovaries of Drosophila melanogaster. However, nothing is known about the role of the Hippo pathway in primitive panoistic ovaries. This work examines the mRNA expression levels of the main components of the Hippo pathway in the panoistic ovary of the basal insect species Blattella germanica, and demonstrates the function of Hippo through RNAi. In Hippo-depleted specimens, the follicular cells of the basal ovarian follicles proliferate without arresting cytokinesis; the epithelium therefore becomes bilayered, impairing ovarian follicle growth. This phenotype is accompanied by long stalks between the ovarian follicles. In D. melanogaster loss of function of Notch determines that the stalk is not developed. With this in mind, we tested whether Hippo and Notch pathways are related in B. germanica. In Notch (only)-depleted females, no stalks were formed between the ovarian follicles. Simultaneous depletion of Hippo and Notch rescued partially the stalk to wild-type. Unlike in the meroistic ovaries of D. melanogaster, in panoistic ovaries the Hippo pathway appears to regulate follicular cell proliferation by acting as a repressor of Notch, triggering the switch from mitosis to the endocycle in the follicular cells. The phylogenetically basal position of B. germanica suggests that this might be the ancestral function of Hippo in insect ovaries. Copyright: © 2014 Irles, Piulachs.Peer reviewedPublic Library of ScienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/115747reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1371/journal.pone.0113850Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1157472026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
title Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
spellingShingle Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
Irles, Paula
title_short Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
title_full Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
title_fullStr Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
title_full_unstemmed Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
title_sort Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
dc.creator.none.fl_str_mv Irles, Paula
Piulachs, Maria-Dolors
author Irles, Paula
author_facet Irles, Paula
Piulachs, Maria-Dolors
author_role author
author2 Piulachs, Maria-Dolors
author2_role author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description During insect oogenesis, the follicular epithelium undergoes both cell proliferation and apoptosis, thus modulating ovarian follicle growth. The Hippo pathway is key in these processes, and has been thoroughly studied in the meroistic ovaries of Drosophila melanogaster. However, nothing is known about the role of the Hippo pathway in primitive panoistic ovaries. This work examines the mRNA expression levels of the main components of the Hippo pathway in the panoistic ovary of the basal insect species Blattella germanica, and demonstrates the function of Hippo through RNAi. In Hippo-depleted specimens, the follicular cells of the basal ovarian follicles proliferate without arresting cytokinesis; the epithelium therefore becomes bilayered, impairing ovarian follicle growth. This phenotype is accompanied by long stalks between the ovarian follicles. In D. melanogaster loss of function of Notch determines that the stalk is not developed. With this in mind, we tested whether Hippo and Notch pathways are related in B. germanica. In Notch (only)-depleted females, no stalks were formed between the ovarian follicles. Simultaneous depletion of Hippo and Notch rescued partially the stalk to wild-type. Unlike in the meroistic ovaries of D. melanogaster, in panoistic ovaries the Hippo pathway appears to regulate follicular cell proliferation by acting as a repressor of Notch, triggering the switch from mitosis to the endocycle in the follicular cells. The phylogenetically basal position of B. germanica suggests that this might be the ancestral function of Hippo in insect ovaries. Copyright: © 2014 Irles, Piulachs.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/115747
url http://hdl.handle.net/10261/115747
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0113850
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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