Pneumocystis jirovecii among patients with cystic fibrosis and their household members

We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10...

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Autores: Morilla Romero de la Osa, R., Medrano, Francisco Javier, Calzada Valle, A., Quintana Gallego, María Esther, Campano Cuevas, E., Friaza Patiño, Vicente, Calderón, Enrique J., Horra, Carmen de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/269699
Acceso en línea:http://hdl.handle.net/10261/269699
Access Level:acceso abierto
Palabra clave:Pneumocystis jirovecii
Disease transmission
Cystic fibrosis
Polymerase chain reaction
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spelling Pneumocystis jirovecii among patients with cystic fibrosis and their household membersMorilla Romero de la Osa, R.Medrano, Francisco JavierCalzada Valle, A.Quintana Gallego, María EstherCampano Cuevas, E.Friaza Patiño, VicenteCalderón, Enrique J.Horra, Carmen de laPneumocystis jiroveciiDisease transmissionCystic fibrosisPolymerase chain reactionWe conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection.The study was supported, in part, by the Red Iberoamericana sobre Pneumocystosis in the framework of The Ibero-American Programme for Science, Technology and Development (grant CYTED 212RT0450).Oxford University PressRed Iberoamericana sobre PneumocystosisPrograma Iberoamericano de Ciencia y Tecnología para el DesarrolloConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/269699reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/mmy/myab010Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2696992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Pneumocystis jirovecii among patients with cystic fibrosis and their household members
title Pneumocystis jirovecii among patients with cystic fibrosis and their household members
spellingShingle Pneumocystis jirovecii among patients with cystic fibrosis and their household members
Morilla Romero de la Osa, R.
Pneumocystis jirovecii
Disease transmission
Cystic fibrosis
Polymerase chain reaction
title_short Pneumocystis jirovecii among patients with cystic fibrosis and their household members
title_full Pneumocystis jirovecii among patients with cystic fibrosis and their household members
title_fullStr Pneumocystis jirovecii among patients with cystic fibrosis and their household members
title_full_unstemmed Pneumocystis jirovecii among patients with cystic fibrosis and their household members
title_sort Pneumocystis jirovecii among patients with cystic fibrosis and their household members
dc.creator.none.fl_str_mv Morilla Romero de la Osa, R.
Medrano, Francisco Javier
Calzada Valle, A.
Quintana Gallego, María Esther
Campano Cuevas, E.
Friaza Patiño, Vicente
Calderón, Enrique J.
Horra, Carmen de la
author Morilla Romero de la Osa, R.
author_facet Morilla Romero de la Osa, R.
Medrano, Francisco Javier
Calzada Valle, A.
Quintana Gallego, María Esther
Campano Cuevas, E.
Friaza Patiño, Vicente
Calderón, Enrique J.
Horra, Carmen de la
author_role author
author2 Medrano, Francisco Javier
Calzada Valle, A.
Quintana Gallego, María Esther
Campano Cuevas, E.
Friaza Patiño, Vicente
Calderón, Enrique J.
Horra, Carmen de la
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Red Iberoamericana sobre Pneumocystosis
Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pneumocystis jirovecii
Disease transmission
Cystic fibrosis
Polymerase chain reaction
topic Pneumocystis jirovecii
Disease transmission
Cystic fibrosis
Polymerase chain reaction
description We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/269699
url http://hdl.handle.net/10261/269699
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/mmy/myab010
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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