Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species

Dendritic spines have diverse morphologies, with a wide range of head and neck sizes, and these morphologic differences likely generate different functional properties. To explore how this morphologic diversity differs across species and ages we analyzed 3D confocal reconstructions of;8000 human spi...

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Detalles Bibliográficos
Autores: Ofer, N., Benavides-Piccione, Ruth, DeFelipe, Javier, Yuste, Rafael
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::dab0bfaddcab2ff52a882e8a307e01ed
Acceso en línea:http://hdl.handle.net/10261/279510
Access Level:acceso abierto
Palabra clave:3D confocal reconstructions
cerebral cortex
plasticity
pyramidal cells.
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spelling Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and SpeciesOfer, N.Benavides-Piccione, RuthDeFelipe, JavierYuste, Rafael3D confocal reconstructionscerebral cortexplasticitypyramidal cells.Dendritic spines have diverse morphologies, with a wide range of head and neck sizes, and these morphologic differences likely generate different functional properties. To explore how this morphologic diversity differs across species and ages we analyzed 3D confocal reconstructions of;8000 human spines and;1700 mouse spines, labeled by intracellular injections in fixed tissue. Using unsupervised algorithms, we computa-tionally separated spine heads and necks and systematically measured morphologic features of spines in apical and basal dendrites from cortical pyramidal cells. Human spines had unimodal distributions of parameters, without any evidence of morphologic subtypes. Their spine necks were longer and thinner in apical than in basal spines, and spine head volumes of an 85-year-old individual were larger than those of a 40-year-old in-dividual. Human spines had longer and thicker necks and larger head volumes than mouse spines. Our results indicate that human spines form part of a continuum, are larger and longer than those of mice, and become larger with increasing adult age. These morphologic differences in spines across species could generate functional differences in biochemical and electrical spine compartmentalization, or in synaptic properties, across species and ages.This work was supported by National Institute of Neurological Disorders and Stroke Grants R01NS110422 and R34NS116740 and the National Institute of Mental Health Grant R01MH115900.Society for NeuroscienceNational Institute of Neurological Disorders and Stroke (US)National Institute of Mental Health (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/279510reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1523/ENEURO.0039-22.2022Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::dab0bfaddcab2ff52a882e8a307e01ed2026-05-22T06:33:51Z
dc.title.none.fl_str_mv Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
title Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
spellingShingle Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
Ofer, N.
3D confocal reconstructions
cerebral cortex
plasticity
pyramidal cells.
title_short Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
title_full Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
title_fullStr Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
title_full_unstemmed Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
title_sort Structural Analysis of Human and Mouse Dendritic Spines Reveals a Morphological Continuum and Differences across Ages and Species
dc.creator.none.fl_str_mv Ofer, N.
Benavides-Piccione, Ruth
DeFelipe, Javier
Yuste, Rafael
author Ofer, N.
author_facet Ofer, N.
Benavides-Piccione, Ruth
DeFelipe, Javier
Yuste, Rafael
author_role author
author2 Benavides-Piccione, Ruth
DeFelipe, Javier
Yuste, Rafael
author2_role author
author
author
dc.contributor.none.fl_str_mv National Institute of Neurological Disorders and Stroke (US)
National Institute of Mental Health (US)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 3D confocal reconstructions
cerebral cortex
plasticity
pyramidal cells.
topic 3D confocal reconstructions
cerebral cortex
plasticity
pyramidal cells.
description Dendritic spines have diverse morphologies, with a wide range of head and neck sizes, and these morphologic differences likely generate different functional properties. To explore how this morphologic diversity differs across species and ages we analyzed 3D confocal reconstructions of;8000 human spines and;1700 mouse spines, labeled by intracellular injections in fixed tissue. Using unsupervised algorithms, we computa-tionally separated spine heads and necks and systematically measured morphologic features of spines in apical and basal dendrites from cortical pyramidal cells. Human spines had unimodal distributions of parameters, without any evidence of morphologic subtypes. Their spine necks were longer and thinner in apical than in basal spines, and spine head volumes of an 85-year-old individual were larger than those of a 40-year-old in-dividual. Human spines had longer and thicker necks and larger head volumes than mouse spines. Our results indicate that human spines form part of a continuum, are larger and longer than those of mice, and become larger with increasing adult age. These morphologic differences in spines across species could generate functional differences in biochemical and electrical spine compartmentalization, or in synaptic properties, across species and ages.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/279510
url http://hdl.handle.net/10261/279510
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1523/ENEURO.0039-22.2022
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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