Ebola virus-like particles as potential vaccine candidates

Ebola virus causes outbreaks with high case-fatality rates, and current vaccines face limitations such as dependence on viral vectors, weak cellular immunity, and cold-chain requirements. Virus-like particles and extracellular vesicles are promising vaccine platforms, but downstream processing chall...

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Detalhes bibliográficos
Autor: Mellid Carballal, Rocío
Formato: tesis doctoral
Fecha de publicación:2026
País:España
Recursos:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:dnet:minerva_____::fe5ee9a57aa99cb4fe84f88181a99603
Acesso em linha:https://hdl.handle.net/10347/46410
Access Level:acceso abierto
Palavra-chave:Ebola virus
virus-like particles
extracellular vesicles
vaccines
purification
330290 Ingeniería bioquímica
241210 Vacunas
Descrição
Resumo:Ebola virus causes outbreaks with high case-fatality rates, and current vaccines face limitations such as dependence on viral vectors, weak cellular immunity, and cold-chain requirements. Virus-like particles and extracellular vesicles are promising vaccine platforms, but downstream processing challenges limit their clinical translation. This thesis focuses on the production, purification, and immunological evaluation of Ebola VP40-derived virus-like particles (eVLPs) and extracellular vesicles (eEVs), demonstrating their distinct ability to induce dendritic cell maturation. Additionally, two strategies are explored to enhance the immunogenicity of eVLPs. Overall, this work underscores the importance of optimizing purification processes and highlights the potential of eVLPs as vaccine candidates against Ebola virus.