Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms

The control of infectious diseases is a major current challenge in intensive aquaculture. Most commercial vaccines are based on live attenuated or inactivated pathogens that are usually combined with adjuvants, oil emulsions being as the most widely used for vaccination in aquaculture. Although effe...

Descripción completa

Detalles Bibliográficos
Autores: Torrealba, Débora, Seras-Franzoso, Joaquin, Mamat, Uwe, Wilke, Kathleen, Villaverde, Antonio, Roher, Nerea, Garcia-Fruitos, Elena
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1802
Acceso en línea:http://hdl.handle.net/20.500.12327/1802
https://doi.org/10.1371/journal.pone.0164073
Access Level:acceso abierto
Palabra clave:619
id ES_a871c485bbc289c2d287c172c81b5ebf
oai_identifier_str oai:recercat.cat:20.500.12327/1802
network_acronym_str ES
network_name_str España
repository_id_str
spelling Complex Particulate Biomaterials as Immunostimulant-Delivery PlatformsTorrealba, DéboraSeras-Franzoso, JoaquinMamat, UweWilke, KathleenVillaverde, AntonioRoher, NereaGarcia-Fruitos, Elena619The control of infectious diseases is a major current challenge in intensive aquaculture. Most commercial vaccines are based on live attenuated or inactivated pathogens that are usually combined with adjuvants, oil emulsions being as the most widely used for vaccination in aquaculture. Although effective, the use of these oil emulsions is plagued with important side effects. Thus, the development of alternative safer and cost-effective immunostimulants and adjuvants is highly desirable. Here we have explored the capacity of inclusion bodies produced in bacteria to immunostimulate and protect fish against bacterial infections. Bacterial inclusion bodies are highly stable, non-toxic protein-based biomaterials produced through fully scalable and low-cost bio-production processes. The present study shows that the composition and structured organization of inclusion body components (protein, lipopolysaccharide, peptidoglycan, DNA and RNA) make these protein biomaterials excellent immunomodulators able to generically protect fish against otherwise lethal bacterial challenges. The results obtained in this work provide evidence that their inherent nature makes bacterial inclusion bodies exceptionally attractive as immunostimulants and this opens the door to the future exploration of this biomaterial as an alternative adjuvant for vaccination purposes in veterinary.info:eu-repo/semantics/publishedVersionPublic Library of ScienceProducció AnimalProducció de Remugants2016info:eu-repo/semantics/article17http://hdl.handle.net/20.500.12327/1802https://doi.org/10.1371/journal.pone.0164073reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS ONEMINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-33877/ES/DESARROLLO DE NANOLIPOSOMAS COMO VEHICULOS DE INMUNOESTIMULANTES-VACUNAS EN ESPECIES DE INTERES PARA LA ACUICULTURA/MINECOPrograma Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2010-06210/ES/ /Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/18022026-05-29T05:05:01Z
dc.title.none.fl_str_mv Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
title Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
spellingShingle Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
Torrealba, Débora
619
title_short Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
title_full Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
title_fullStr Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
title_full_unstemmed Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
title_sort Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
dc.creator.none.fl_str_mv Torrealba, Débora
Seras-Franzoso, Joaquin
Mamat, Uwe
Wilke, Kathleen
Villaverde, Antonio
Roher, Nerea
Garcia-Fruitos, Elena
author Torrealba, Débora
author_facet Torrealba, Débora
Seras-Franzoso, Joaquin
Mamat, Uwe
Wilke, Kathleen
Villaverde, Antonio
Roher, Nerea
Garcia-Fruitos, Elena
author_role author
author2 Seras-Franzoso, Joaquin
Mamat, Uwe
Wilke, Kathleen
Villaverde, Antonio
Roher, Nerea
Garcia-Fruitos, Elena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Producció de Remugants
dc.subject.none.fl_str_mv 619
topic 619
description The control of infectious diseases is a major current challenge in intensive aquaculture. Most commercial vaccines are based on live attenuated or inactivated pathogens that are usually combined with adjuvants, oil emulsions being as the most widely used for vaccination in aquaculture. Although effective, the use of these oil emulsions is plagued with important side effects. Thus, the development of alternative safer and cost-effective immunostimulants and adjuvants is highly desirable. Here we have explored the capacity of inclusion bodies produced in bacteria to immunostimulate and protect fish against bacterial infections. Bacterial inclusion bodies are highly stable, non-toxic protein-based biomaterials produced through fully scalable and low-cost bio-production processes. The present study shows that the composition and structured organization of inclusion body components (protein, lipopolysaccharide, peptidoglycan, DNA and RNA) make these protein biomaterials excellent immunomodulators able to generically protect fish against otherwise lethal bacterial challenges. The results obtained in this work provide evidence that their inherent nature makes bacterial inclusion bodies exceptionally attractive as immunostimulants and this opens the door to the future exploration of this biomaterial as an alternative adjuvant for vaccination purposes in veterinary.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1802
https://doi.org/10.1371/journal.pone.0164073
url http://hdl.handle.net/20.500.12327/1802
https://doi.org/10.1371/journal.pone.0164073
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-33877/ES/DESARROLLO DE NANOLIPOSOMAS COMO VEHICULOS DE INMUNOESTIMULANTES-VACUNAS EN ESPECIES DE INTERES PARA LA ACUICULTURA/
MINECOPrograma Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/
MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2010-06210/ES/ /
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415879905640448
score 15,812455

Similares en LA Referencia