The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization

13 Pág.

Detalles Bibliográficos
Autores: Gonzalez-Klein, Zulema, Cuevas-Zuviría, Bruno, Wangorsch, Andrea, Hernandez-Ramirez, Guadalupe, Pazos-Castro, Diego, Oeo-Santos, Carmen, Romero-Sahagun, Alejandro, Pacios, Luis F., Tome-Amat, Jaime, Scheurer, Stephan, Díaz-Perales, Araceli, Garrido-Arandia, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372475
Acceso en línea:http://hdl.handle.net/10261/372475
https://api.elsevier.com/content/abstract/scopus_id/85105307795
Access Level:acceso abierto
Palabra clave:Food allergy
Ligand
Lipid transfer protein
Phytosphingosine
id ES_a85bce2d9d40cdfb5457ad8fb5869841
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
title The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
spellingShingle The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
Gonzalez-Klein, Zulema
Food allergy
Ligand
Lipid transfer protein
Phytosphingosine
title_short The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
title_full The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
title_fullStr The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
title_full_unstemmed The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
title_sort The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization
dc.creator.none.fl_str_mv Gonzalez-Klein, Zulema
Cuevas-Zuviría, Bruno
Wangorsch, Andrea
Hernandez-Ramirez, Guadalupe
Pazos-Castro, Diego
Oeo-Santos, Carmen
Romero-Sahagun, Alejandro
Pacios, Luis F.
Tome-Amat, Jaime
Scheurer, Stephan
Díaz-Perales, Araceli
Garrido-Arandia, María
author Gonzalez-Klein, Zulema
author_facet Gonzalez-Klein, Zulema
Cuevas-Zuviría, Bruno
Wangorsch, Andrea
Hernandez-Ramirez, Guadalupe
Pazos-Castro, Diego
Oeo-Santos, Carmen
Romero-Sahagun, Alejandro
Pacios, Luis F.
Tome-Amat, Jaime
Scheurer, Stephan
Díaz-Perales, Araceli
Garrido-Arandia, María
author_role author
author2 Cuevas-Zuviría, Bruno
Wangorsch, Andrea
Hernandez-Ramirez, Guadalupe
Pazos-Castro, Diego
Oeo-Santos, Carmen
Romero-Sahagun, Alejandro
Pacios, Luis F.
Tome-Amat, Jaime
Scheurer, Stephan
Díaz-Perales, Araceli
Garrido-Arandia, María
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Economía y Competitividad (España)
Instituto de Salud Carlos III
European Commission
Comunidad de Madrid
Universidad Politécnica de Madrid
Gonzalez-Klein, Zulema [0000-0003-3762-3781]
Cuevas-Zuviría, Bruno [0000-0003-1479-9442]
Wangorsch, Andrea [0000-0002-5184-5455]
Hernandez-Ramirez, Guadalupe [0000-0003-3031-6754]
Pazos-Castro, Diego [0000-0002-0686-5131]
Oeo-Santos, Carmen [0000-0002-2431-4499]
Romero-Sahagun, Alejandro [0000-0002-9417-8293]
Pacios, Luis F. [0000-0002-0585-4289]
Tome-Amat, Jaime [0000-0003-4442-3649]
Scheurer, Stephan [0000-0002-2859-562X]
Díaz-Perales, Araceli [0000-0002-1093-3627]
Garrido-Arandia, María [0000-0001-6114-5754]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Food allergy
Ligand
Lipid transfer protein
Phytosphingosine
topic Food allergy
Ligand
Lipid transfer protein
Phytosphingosine
description 13 Pág.
publishDate 2021
dc.date.none.fl_str_mv 2021
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372475
https://api.elsevier.com/content/abstract/scopus_id/85105307795
url http://hdl.handle.net/10261/372475
https://api.elsevier.com/content/abstract/scopus_id/85105307795
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-84548-R
S2018/BAAA-4574
info:eu-repo/grantAgreement/EC/H2020/768641
Centro de Biotecnología y Genómica de Plantas (CBGP)
publisher
https://doi.org/10.1016/j.bbalip.2021.158928
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415872564559872
spelling The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterizationGonzalez-Klein, ZulemaCuevas-Zuviría, BrunoWangorsch, AndreaHernandez-Ramirez, GuadalupePazos-Castro, DiegoOeo-Santos, CarmenRomero-Sahagun, AlejandroPacios, Luis F.Tome-Amat, JaimeScheurer, StephanDíaz-Perales, AraceliGarrido-Arandia, MaríaFood allergyLigandLipid transfer proteinPhytosphingosine13 Pág.Plant lipid transfer proteins are a large family that can be found in all land plants. They have a hydrophobic cavity that allows them to harbor lipids and facilitates their traffic between membranes. However, in humans, this plant protein family is responsible for the main food allergies in the Mediterranean area. Nevertheless, not only the protein itself but also its ligand is relevant for allergic sensitization. The main aim of the present work is to analyse the natural ligands carried by four allergenic LTPs (Tri a 14, Art v 3, Par j 2, and Ole e 7), compared with the previously identified ligand of Pru p 3 (CPT-PHS ligand), and clarify their role within the immunological reactions. Results showed that the ligands of the LTPs studied shared a chemical identity, in which the presence of a polar head was essential to the protein-ligand binding. This ligand was transported through a skin cellular model, and phosphorylated phytosphingosine could be detected as result of cell metabolism. Since sphingosine kinase 1 was overexpressed in keratinocytes incubated with the LTP-ligand complex, this enzyme might be responsible for the phosphorylation of the phytosphingosine fraction of the CPT-PHS ligand. This way, phytosphingosine-1-phosphate could be mimicking the role of the human inflammatory mediator sphingosine-1-phosphate, explaining why LTPs are associated with more severe allergic responses. In conclusion, this work contributes to the understanding of the chemical nature and behavior of lipid ligands carried by allergens, which would help to gain insight into their role during allergic sensitization.This research was funded by the Spanish Government (MINECO, grant BIO2017-84548- R); Instituto de Salud Carlos III (ISCIII) co-founded by FEDER Thematic Networks and Cooperative Research Centers: ARADYAL (RD16/0006/0003), the Community of Madrid (FOODAL-CM_S2018/BAAA-4574), European Commission (H2020-NMBP-X-KET-2017 - 768641 – AllerScreening) and Universidad Politécnica de Madrid and Banco Santander for a predoctoral Programa Propio grant.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Economía y Competitividad (España)Instituto de Salud Carlos IIIEuropean CommissionComunidad de MadridUniversidad Politécnica de MadridGonzalez-Klein, Zulema [0000-0003-3762-3781]Cuevas-Zuviría, Bruno [0000-0003-1479-9442]Wangorsch, Andrea [0000-0002-5184-5455]Hernandez-Ramirez, Guadalupe [0000-0003-3031-6754]Pazos-Castro, Diego [0000-0002-0686-5131]Oeo-Santos, Carmen [0000-0002-2431-4499]Romero-Sahagun, Alejandro [0000-0002-9417-8293]Pacios, Luis F. [0000-0002-0585-4289]Tome-Amat, Jaime [0000-0003-4442-3649]Scheurer, Stephan [0000-0002-2859-562X]Díaz-Perales, Araceli [0000-0002-1093-3627]Garrido-Arandia, María [0000-0001-6114-5754]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/372475https://api.elsevier.com/content/abstract/scopus_id/85105307795reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-84548-RS2018/BAAA-4574info:eu-repo/grantAgreement/EC/H2020/768641Centro de Biotecnología y Genómica de Plantas (CBGP)publisherhttps://doi.org/10.1016/j.bbalip.2021.158928Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3724752026-05-22T06:33:51Z
score 15,198674

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