Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells

Background: In physiological conditions, it is postulated that neurons control microglial reactivity through a series of inhibitory mechanisms, involving either cell contact-dependent, soluble-factor-dependent or neurotransmitter-associated pathways. In the current study, we focus on CD200R1, a micr...

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Autores: Dentesano, Guido, Straccia, Marco, Ejarque Ortiz, Aroa, Tusell Puigbert, José Ma., Serratosa i Serdà, Joan, Saura Martí, Josep, Solà i Subirana, Carme
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/101598
Acceso en línea:https://hdl.handle.net/2445/101598
Access Level:acceso abierto
Palabra clave:Neurociències
Neurobiologia
Neuròglia
Teixit nerviós
Cultiu de teixits
Neurosciences
Neurobiology
Neuroglia
Nerve tissue
Tissue culture
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network_name_str España
repository_id_str
spelling Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cellsDentesano, GuidoStraccia, MarcoEjarque Ortiz, AroaTusell Puigbert, José Ma.Serratosa i Serdà, JoanSaura Martí, JosepSolà i Subirana, CarmeNeurociènciesNeurobiologiaNeurògliaTeixit nerviósCultiu de teixitsNeurosciencesNeurobiologyNeurogliaNerve tissueTissue cultureBackground: In physiological conditions, it is postulated that neurons control microglial reactivity through a series of inhibitory mechanisms, involving either cell contact-dependent, soluble-factor-dependent or neurotransmitter-associated pathways. In the current study, we focus on CD200R1, a microglial receptor involved in one of these cell contact-dependent mechanisms. CD200R1 activation by its ligand, CD200 (mainly expressed by neurons in the central nervous system),is postulated to inhibit the pro-inflammatory phenotype of microglial cells, while alterations in CD200-CD200R1 signalling potentiate this phenotype. Little is known about the regulation of CD200R1 expression in microglia or possible alterations in the presence of pro-inflammatory stimuli. Methods: Murine primary microglial cultures, mixed glial cultures from wild-type and CCAAT/enhancer binding protein β (C/EBPβ)-deficient mice, and the BV2 murine cell line overexpressing C/EBPβ were used to study the involvement of C/EBPβ transcription factor in the regulation of CD200R1 expression in response to a proinflammatory stimulus (lipopolysaccharide (LPS)). Binding of C/EBPβ to the CD200R1 promoter was determined by quantitative chromatin immunoprecipitation (qChIP). The involvement of histone deacetylase 1 in the control of CD200R1 expression by C/EBPβ was also determined by co-immunoprecipitation and qChIP. Results: LPS treatment induced a decrease in CD200R1 mRNA and protein expression in microglial cells, an effect that was not observed in the absence of C/EBPβ. C/EBPβ overexpression in BV2 cells resulted in a decrease in basal CD200R1 mRNA and protein expression. In addition, C/EBPβ binding to the CD200R1 promoter was observed in LPS-treated but not in control glial cells, and also in control BV2 cells overexpressing C/EBPβ. Finally, we observed that histone deacetylase 1 co-immunoprecipitated with C/EBPβ and showed binding to a C/EBPβ consensus sequence of the CD200R1 promoter in LPS-treated glial cells. Moreover, histone deacetylase 1 inhibitors reversed the decrease in CD200R1 expression induced by LPS treatment. Conclusions: CD200R1 expression decreases in microglial cells in the presence of a pro-inflammatory stimulus, an effect that is regulated, at least in part, by C/EBPβ. Histone deacetylase 1 may mediate C/EBPβ inhibition of CD200R1 expression, through a direct effect on C/EBPβ transcriptional activity and/or on chromatin structure.BioMed Central2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/101598Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/1742-2094-9-165Journal of Neuroinflammation, 2012, vol. 9, num. 165http://dx.doi.org/10.1186/1742-2094-9-165cc-by (c) Dentesano, Guido et al., 2012http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1015982026-05-27T06:46:51Z
dc.title.none.fl_str_mv Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
title Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
spellingShingle Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
Dentesano, Guido
Neurociències
Neurobiologia
Neuròglia
Teixit nerviós
Cultiu de teixits
Neurosciences
Neurobiology
Neuroglia
Nerve tissue
Tissue culture
title_short Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
title_full Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
title_fullStr Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
title_full_unstemmed Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
title_sort Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
dc.creator.none.fl_str_mv Dentesano, Guido
Straccia, Marco
Ejarque Ortiz, Aroa
Tusell Puigbert, José Ma.
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
author Dentesano, Guido
author_facet Dentesano, Guido
Straccia, Marco
Ejarque Ortiz, Aroa
Tusell Puigbert, José Ma.
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
author_role author
author2 Straccia, Marco
Ejarque Ortiz, Aroa
Tusell Puigbert, José Ma.
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurociències
Neurobiologia
Neuròglia
Teixit nerviós
Cultiu de teixits
Neurosciences
Neurobiology
Neuroglia
Nerve tissue
Tissue culture
topic Neurociències
Neurobiologia
Neuròglia
Teixit nerviós
Cultiu de teixits
Neurosciences
Neurobiology
Neuroglia
Nerve tissue
Tissue culture
description Background: In physiological conditions, it is postulated that neurons control microglial reactivity through a series of inhibitory mechanisms, involving either cell contact-dependent, soluble-factor-dependent or neurotransmitter-associated pathways. In the current study, we focus on CD200R1, a microglial receptor involved in one of these cell contact-dependent mechanisms. CD200R1 activation by its ligand, CD200 (mainly expressed by neurons in the central nervous system),is postulated to inhibit the pro-inflammatory phenotype of microglial cells, while alterations in CD200-CD200R1 signalling potentiate this phenotype. Little is known about the regulation of CD200R1 expression in microglia or possible alterations in the presence of pro-inflammatory stimuli. Methods: Murine primary microglial cultures, mixed glial cultures from wild-type and CCAAT/enhancer binding protein β (C/EBPβ)-deficient mice, and the BV2 murine cell line overexpressing C/EBPβ were used to study the involvement of C/EBPβ transcription factor in the regulation of CD200R1 expression in response to a proinflammatory stimulus (lipopolysaccharide (LPS)). Binding of C/EBPβ to the CD200R1 promoter was determined by quantitative chromatin immunoprecipitation (qChIP). The involvement of histone deacetylase 1 in the control of CD200R1 expression by C/EBPβ was also determined by co-immunoprecipitation and qChIP. Results: LPS treatment induced a decrease in CD200R1 mRNA and protein expression in microglial cells, an effect that was not observed in the absence of C/EBPβ. C/EBPβ overexpression in BV2 cells resulted in a decrease in basal CD200R1 mRNA and protein expression. In addition, C/EBPβ binding to the CD200R1 promoter was observed in LPS-treated but not in control glial cells, and also in control BV2 cells overexpressing C/EBPβ. Finally, we observed that histone deacetylase 1 co-immunoprecipitated with C/EBPβ and showed binding to a C/EBPβ consensus sequence of the CD200R1 promoter in LPS-treated glial cells. Moreover, histone deacetylase 1 inhibitors reversed the decrease in CD200R1 expression induced by LPS treatment. Conclusions: CD200R1 expression decreases in microglial cells in the presence of a pro-inflammatory stimulus, an effect that is regulated, at least in part, by C/EBPβ. Histone deacetylase 1 may mediate C/EBPβ inhibition of CD200R1 expression, through a direct effect on C/EBPβ transcriptional activity and/or on chromatin structure.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/101598
url https://hdl.handle.net/2445/101598
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/1742-2094-9-165
Journal of Neuroinflammation, 2012, vol. 9, num. 165
http://dx.doi.org/10.1186/1742-2094-9-165
dc.rights.none.fl_str_mv cc-by (c) Dentesano, Guido et al., 2012
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Dentesano, Guido et al., 2012
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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