Microrheometer for Biofluidic Analysis

The motivation for this study was to develop a microdevice for the precise rheological characterization of biofluids, especially blood. The method presented was based on the principles of rheometry and fluid mechanics at the microscale. Traditional rheometers require a considerable amount of space,...

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Detalles Bibliográficos
Autores: Méndez-Mora, Lourdes|||0000-0001-9637-459X, Cabello-Fusarés, Maria, Ferré-Torres, Josep, Riera-Llobet, Carla|||0000-0002-9037-1539, López, Samantha, Trejo-Soto, Claudia|||0000-0003-0267-3359, Alarcón Cor, Tomás|||0000-0002-8566-3676, Hernández-Machado, Aurora|||0000-0002-0397-5255
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:247499
Acceso en línea:https://ddd.uab.cat/record/247499
https://dx.doi.org/urn:doi:10.3390/mi12060726
Access Level:acceso abierto
Palabra clave:Rheometer
Microrheometer
Rheology
Hemorheology
Viscosity
Blood
Plasma
Descripción
Sumario:The motivation for this study was to develop a microdevice for the precise rheological characterization of biofluids, especially blood. The method presented was based on the principles of rheometry and fluid mechanics at the microscale. Traditional rheometers require a considerable amount of space, are expensive, and require a large volume of sample. A mathematical model was developed that, combined with a proper experimental model, allowed us to characterize the viscosity of Newtonian and non-Newtonian fluids at different shear rates. The technology presented here is the basis of a point-of-care device capable of describing the nonlinear rheology of biofluids by the fluid/air interface front velocity characterization through a microchannel. The proposed microrheometer uses a small amount of sample to deliver fast and accurate results, without needing a large laboratory space. Blood samples from healthy donors at distinct hematocrit percentages were the non-Newtonian fluid selected for the study. Water and plasma were employed as testing Newtonian fluids for validation of the system. The viscosity results obtained for the Newtonian and non-Newtonian fluids were consistent with pertinent studies cited in this paper. In addition, the results achieved using the proposed method allowed distinguishing between blood samples with different characteristics.