The early development of the inner ear: the role of notch and fgf pathways in aerly otic neural versus non-neural patterning

Otic neuronal precursors are specified in the otic placode but interestingly do so only in the anterior domain of the otic placode, the proneural domain. In the present study, we have explored why only this territory has the competence to undergo neurogenesis, this means the early events of otic pro...

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Detalles Bibliográficos
Autor: Abelló Sumpsi, Gina
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/7121
Acceso en línea:http://www.tdx.cat/TDX-0401109-114317
http://hdl.handle.net/10803/7121
Access Level:acceso abierto
Palabra clave:ratolí
peix
pollet
embrió
cultius d'embrió
electroporació
marcatge cellular
Sox3
resctricció de llinatges
Notch
FGF
territori no-neural
neurona
territori proneural
regionalització
oída
Desenvolupament
lineage restrictions
embryo culturing
cell labelling
electroporation
DiI
mouse
zebrafish
chick
embryo
otic placode
neurogenesis
DAPT
patterning
signaling
proneural
Development
612
Descripción
Sumario:Otic neuronal precursors are specified in the otic placode but interestingly do so only in the anterior domain of the otic placode, the proneural domain. In the present study, we have explored why only this territory has the competence to undergo neurogenesis, this means the early events of otic proneural regionalization and neural commitment. The proneural and non-neural domains presented complementary gene expression patterns of transcription factors and members of the Notch pathway. Overall, we propose that proneural character is acquired in the anterior territory by the action of localized ectodermal FGF8-FGF10 signaling that enhances Sox3 function. FGF signals through Sox3 activity would be essential for the specification of the proneural domain versus a non-neural territory, while Notch would be involved in refining this early regionalization.