Neonatal exposure to androgens dynamically alters gut microbiota architecture

Gonadal steroids strongly contribute to the metabolic programming that shapes the susceptibility to the manifestation of diseases later in life, and the effect is often sexually dimorphic. Microbiome signatures, together with metabolic traits and sex steroid levels, were analyzed at adulthood in neo...

ver descrição completa

Detalhes bibliográficos
Autores: Barroso, Alexia, Santos Marcos, José A., Perdices López, Cecilia, Vega Rojas, Ana, Sánchez Garrido, Miguel Ángel, Krylova, Yelizabeta, Molina Abril, Helena, Ohlsson, Claes, Pérez Martínez, Pablo, Poutanen, Matti, López Miranda, José, Tena Sempere, Manuel, Camargo, Antonio
Formato: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2020
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/114715
Acesso em linha:https://hdl.handle.net/11441/114715
https://doi.org/10.1530/JOE-20-0277
Access Level:acceso abierto
Palavra-chave:Gut microbiota
Hormones
Sex steroids
Metabolic diseases
Obesity
id ES_a80fa44dc167bf168eda7b36a1430f13
oai_identifier_str oai:idus.us.es:11441/114715
network_acronym_str ES
network_name_str España
repository_id_str
spelling Neonatal exposure to androgens dynamically alters gut microbiota architectureBarroso, AlexiaSantos Marcos, José A.Perdices López, CeciliaVega Rojas, AnaSánchez Garrido, Miguel ÁngelKrylova, YelizabetaMolina Abril, HelenaOhlsson, ClaesPérez Martínez, PabloPoutanen, MattiLópez Miranda, JoséTena Sempere, ManuelCamargo, AntonioGut microbiotaHormonesSex steroidsMetabolic diseasesObesityGonadal steroids strongly contribute to the metabolic programming that shapes the susceptibility to the manifestation of diseases later in life, and the effect is often sexually dimorphic. Microbiome signatures, together with metabolic traits and sex steroid levels, were analyzed at adulthood in neonatally androgenized female rats, and compared with those of control male and female rats. Exposure of female rats to high doses of androgens on early postnatal life resulted in persistent altera tions of the sex steroid profile later on life, namely lower progesterone and higher estr adiol and estrone levels, with no effect on endogenous androgens. Neonatally androgenized females were heavier (10% at early adulthood and 26% at adulthood) than controls and had impaired glucose homeostasis observed by higher AUC of glucose in GTT and ITT when subjected to obesogenic manipulations. Androgenized female displayed overt alterations in gut microbiota, indicated especially by higher Bacteroidetes and lower Firmicutes abundance at early adulthood, which disappeared when animals were concurrently overfed at adulthood. Notably, these changes in gut microbiota were related with the intestinal expression of several miRNAs, such as miR-27a-3p, miR-29a-5p, a nd miR-100-3p. Our results suggest that nutritional and hormonal disruption at early developmental periods not only alters the metabolic programming of the individual later in life but also perturbs the architecture of gut microbiota, which may interact with the host by a cross-talk mediated by intestinal miRNAs; phenomena that may contribute to amplify the metabolic derangement caused by obesity, as seen in neonatally androgenized female rats.BioScientificaMatemática Aplicada I2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/114715https://doi.org/10.1530/JOE-20-0277reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Endocrinology, 247 (1), 69-85.https://joe.bioscientifica.com/view/journals/joe/247/1/JOE-20-0277.xmlinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1147152026-06-17T12:51:07Z
dc.title.none.fl_str_mv Neonatal exposure to androgens dynamically alters gut microbiota architecture
title Neonatal exposure to androgens dynamically alters gut microbiota architecture
spellingShingle Neonatal exposure to androgens dynamically alters gut microbiota architecture
Barroso, Alexia
Gut microbiota
Hormones
Sex steroids
Metabolic diseases
Obesity
title_short Neonatal exposure to androgens dynamically alters gut microbiota architecture
title_full Neonatal exposure to androgens dynamically alters gut microbiota architecture
title_fullStr Neonatal exposure to androgens dynamically alters gut microbiota architecture
title_full_unstemmed Neonatal exposure to androgens dynamically alters gut microbiota architecture
title_sort Neonatal exposure to androgens dynamically alters gut microbiota architecture
dc.creator.none.fl_str_mv Barroso, Alexia
Santos Marcos, José A.
Perdices López, Cecilia
Vega Rojas, Ana
Sánchez Garrido, Miguel Ángel
Krylova, Yelizabeta
Molina Abril, Helena
Ohlsson, Claes
Pérez Martínez, Pablo
Poutanen, Matti
López Miranda, José
Tena Sempere, Manuel
Camargo, Antonio
author Barroso, Alexia
author_facet Barroso, Alexia
Santos Marcos, José A.
Perdices López, Cecilia
Vega Rojas, Ana
Sánchez Garrido, Miguel Ángel
Krylova, Yelizabeta
Molina Abril, Helena
Ohlsson, Claes
Pérez Martínez, Pablo
Poutanen, Matti
López Miranda, José
Tena Sempere, Manuel
Camargo, Antonio
author_role author
author2 Santos Marcos, José A.
Perdices López, Cecilia
Vega Rojas, Ana
Sánchez Garrido, Miguel Ángel
Krylova, Yelizabeta
Molina Abril, Helena
Ohlsson, Claes
Pérez Martínez, Pablo
Poutanen, Matti
López Miranda, José
Tena Sempere, Manuel
Camargo, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Matemática Aplicada I
dc.subject.none.fl_str_mv Gut microbiota
Hormones
Sex steroids
Metabolic diseases
Obesity
topic Gut microbiota
Hormones
Sex steroids
Metabolic diseases
Obesity
description Gonadal steroids strongly contribute to the metabolic programming that shapes the susceptibility to the manifestation of diseases later in life, and the effect is often sexually dimorphic. Microbiome signatures, together with metabolic traits and sex steroid levels, were analyzed at adulthood in neonatally androgenized female rats, and compared with those of control male and female rats. Exposure of female rats to high doses of androgens on early postnatal life resulted in persistent altera tions of the sex steroid profile later on life, namely lower progesterone and higher estr adiol and estrone levels, with no effect on endogenous androgens. Neonatally androgenized females were heavier (10% at early adulthood and 26% at adulthood) than controls and had impaired glucose homeostasis observed by higher AUC of glucose in GTT and ITT when subjected to obesogenic manipulations. Androgenized female displayed overt alterations in gut microbiota, indicated especially by higher Bacteroidetes and lower Firmicutes abundance at early adulthood, which disappeared when animals were concurrently overfed at adulthood. Notably, these changes in gut microbiota were related with the intestinal expression of several miRNAs, such as miR-27a-3p, miR-29a-5p, a nd miR-100-3p. Our results suggest that nutritional and hormonal disruption at early developmental periods not only alters the metabolic programming of the individual later in life but also perturbs the architecture of gut microbiota, which may interact with the host by a cross-talk mediated by intestinal miRNAs; phenomena that may contribute to amplify the metabolic derangement caused by obesity, as seen in neonatally androgenized female rats.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/114715
https://doi.org/10.1530/JOE-20-0277
url https://hdl.handle.net/11441/114715
https://doi.org/10.1530/JOE-20-0277
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Endocrinology, 247 (1), 69-85.
https://joe.bioscientifica.com/view/journals/joe/247/1/JOE-20-0277.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415847718551552
score 15,300719

Registos relacionados