Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes

The plasma membrane aquaporin-7 (AQP7) has been shown to be expressed in adipose tissue and its role in glycerol release/uptake in adipocytes has been postulated and correlated with obesity onset. However, some studies have contradicted this view. Based on this situation, we have re-assessed the pre...

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Autores: Madeira, Ana, Camps Camprubí, Marta, Zorzano Olarte, Antonio, Moura, Teresa F., Soveral, Graça
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/124629
Acceso en línea:https://hdl.handle.net/2445/124629
Access Level:acceso abierto
Palabra clave:Cèl·lules animals
Glicerina
Teixit adipós
Animal cells
Glycerin
Adipose tissues
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spelling Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytesMadeira, AnaCamps Camprubí, MartaZorzano Olarte, AntonioMoura, Teresa F.Soveral, GraçaCèl·lules animalsGlicerinaTeixit adipósAnimal cellsGlycerinAdipose tissuesThe plasma membrane aquaporin-7 (AQP7) has been shown to be expressed in adipose tissue and its role in glycerol release/uptake in adipocytes has been postulated and correlated with obesity onset. However, some studies have contradicted this view. Based on this situation, we have re-assessed the precise localization of AQP7 in adipose tissue and analyzed its function as a water and/or glycerol channel in adipose cells. Fractionation of mice adipose tissue revealed that AQP7 is located in both adipose and stromal vascular fractions. Moreover, AQP7 was the only aquaglyceroporin expressed in adipose tissue and in 3T3-L1 adipocytes. By overexpressing the human AQP7 in 3T3-L1 adipocytes it was possible to ascertain its role as a water and glycerol channel in a gain-of-function scenario. AQP7 expression had no effect in equilibrium cell volume but AQP7 loss of function correlated with higher triglyceride content. Furthermore it is also reported for the first time a negative correlation between water permeability and the cell non-osmotic volume supporting the observation that AQP7 depleted cells are more prone to lipid accumulation. Additionally, the strong positive correlation between the rates of water and glycerol transport highlights the role of AQP7 as both a water and a glycerol channel and reflects its expression levels in cells. In all, our results clearly document a direct involvement of AQP7 in water and glycerol transport, as well as in triglyceride content in adipocytes.Public Library of Science (PLoS)2018201820132018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/124629Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0083442PLoS One, 2013, vol. 8, num. 12, p. 1-10https://doi.org/10.1371/journal.pone.0083442cc-by (c) Madeira, Ana et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1246292026-05-29T05:05:01Z
dc.title.none.fl_str_mv Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
title Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
spellingShingle Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
Madeira, Ana
Cèl·lules animals
Glicerina
Teixit adipós
Animal cells
Glycerin
Adipose tissues
title_short Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
title_full Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
title_fullStr Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
title_full_unstemmed Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
title_sort Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
dc.creator.none.fl_str_mv Madeira, Ana
Camps Camprubí, Marta
Zorzano Olarte, Antonio
Moura, Teresa F.
Soveral, Graça
author Madeira, Ana
author_facet Madeira, Ana
Camps Camprubí, Marta
Zorzano Olarte, Antonio
Moura, Teresa F.
Soveral, Graça
author_role author
author2 Camps Camprubí, Marta
Zorzano Olarte, Antonio
Moura, Teresa F.
Soveral, Graça
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules animals
Glicerina
Teixit adipós
Animal cells
Glycerin
Adipose tissues
topic Cèl·lules animals
Glicerina
Teixit adipós
Animal cells
Glycerin
Adipose tissues
description The plasma membrane aquaporin-7 (AQP7) has been shown to be expressed in adipose tissue and its role in glycerol release/uptake in adipocytes has been postulated and correlated with obesity onset. However, some studies have contradicted this view. Based on this situation, we have re-assessed the precise localization of AQP7 in adipose tissue and analyzed its function as a water and/or glycerol channel in adipose cells. Fractionation of mice adipose tissue revealed that AQP7 is located in both adipose and stromal vascular fractions. Moreover, AQP7 was the only aquaglyceroporin expressed in adipose tissue and in 3T3-L1 adipocytes. By overexpressing the human AQP7 in 3T3-L1 adipocytes it was possible to ascertain its role as a water and glycerol channel in a gain-of-function scenario. AQP7 expression had no effect in equilibrium cell volume but AQP7 loss of function correlated with higher triglyceride content. Furthermore it is also reported for the first time a negative correlation between water permeability and the cell non-osmotic volume supporting the observation that AQP7 depleted cells are more prone to lipid accumulation. Additionally, the strong positive correlation between the rates of water and glycerol transport highlights the role of AQP7 as both a water and a glycerol channel and reflects its expression levels in cells. In all, our results clearly document a direct involvement of AQP7 in water and glycerol transport, as well as in triglyceride content in adipocytes.
publishDate 2013
dc.date.none.fl_str_mv 2013
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/124629
url https://hdl.handle.net/2445/124629
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0083442
PLoS One, 2013, vol. 8, num. 12, p. 1-10
https://doi.org/10.1371/journal.pone.0083442
dc.rights.none.fl_str_mv cc-by (c) Madeira, Ana et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Madeira, Ana et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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