Frequently asked questions about chlorophyll fluorescence, the sequel

[EN] Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122: 121-158, 2014a) addressed...

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Detalhes bibliográficos
Autores: Kalaji, Hazem, Schansker, Gert, Brestic, M., Bussotti, Filippo, Calatayud, Angeles, Ferroni, Lorenzo, Goltsev, V., Guidi, Lucia, Jajoo, Anjana, Li, P.M., Losciale, P., Mishra, V., Misra, Amarendra, Pancaldi, S., Penella-Casañ, Consuelo, Pollastrini, M., Suresh, K., Tambussi, E., Yanniccari, M., Zivcak, M., Cetner, M., Zamborska, I.A., Stirbet, A., Olsovska, K., Kunderlikova, K., Shelonzek, H., Rusinowski, S., Baba, W., Nebauer, Sergio G.|||0000-0001-7978-6680
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/135015
Acesso em linha:https://riunet.upv.es/handle/10251/135015
Access Level:acceso abierto
Palavra-chave:Chl a fluorescence
Delayed fluorescence
Photochemical quenching
Energy partitioning
Area
FISIOLOGIA VEGETAL
Descrição
Resumo:[EN] Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122: 121-158, 2014a) addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additionalChl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of F-V/F-M values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, probing the donor side of PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters in QTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answers draw on knowledge fromdifferent Chl a fluorescence analysis domains, yielding in several cases new insights.

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