Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients

Objective To evaluate the clinical utility of the Baveno classification in predicting incident cardiovascular mortality after five years of follow-up in a clinic-based cohort of patients with obstructive sleep apnea (OSA). Materials and Methods We evaluated the reproducibility of the Baveno classifi...

Descripción completa

Detalles Bibliográficos
Autores: Burballa , Carla, Duran, Mònica, Martínez, Cristina, Ariceta ,Gema, Cantero Recasens, Gerard, Meseguer , Anna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/465679
Acceso en línea:https://doi.org/10.1016/j.nefroe.2024.01.016
https://hdl.handle.net/10459.1/465679
Access Level:acceso abierto
Palabra clave:Obstructive sleep apnea
Cardiovascular
Hypoxia
Latino
Precision medicine
Enfermedad de Dent
Exosomas
Vesículas extracelulares
DD1
CLCN5
id ES_a7aa27783988bf9b56ae0c972718fc01
oai_identifier_str oai:repositori.udl.cat:10459.1/465679
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
Aislamiento y caracterización de vesículas extracelulares enriquecidas en exosomas en pacientes españoles con enfermedad de Dent 1
title Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
spellingShingle Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
Burballa , Carla
Obstructive sleep apnea
Cardiovascular
Hypoxia
Latino
Precision medicine
Enfermedad de Dent
Exosomas
Vesículas extracelulares
DD1
CLCN5
title_short Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
title_full Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
title_fullStr Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
title_full_unstemmed Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
title_sort Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patients
dc.creator.none.fl_str_mv Burballa , Carla
Duran, Mònica
Martínez, Cristina
Ariceta ,Gema
Cantero Recasens, Gerard
Meseguer , Anna
author Burballa , Carla
author_facet Burballa , Carla
Duran, Mònica
Martínez, Cristina
Ariceta ,Gema
Cantero Recasens, Gerard
Meseguer , Anna
author_role author
author2 Duran, Mònica
Martínez, Cristina
Ariceta ,Gema
Cantero Recasens, Gerard
Meseguer , Anna
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Obstructive sleep apnea
Cardiovascular
Hypoxia
Latino
Precision medicine
Enfermedad de Dent
Exosomas
Vesículas extracelulares
DD1
CLCN5
topic Obstructive sleep apnea
Cardiovascular
Hypoxia
Latino
Precision medicine
Enfermedad de Dent
Exosomas
Vesículas extracelulares
DD1
CLCN5
description Objective To evaluate the clinical utility of the Baveno classification in predicting incident cardiovascular mortality after five years of follow-up in a clinic-based cohort of patients with obstructive sleep apnea (OSA). Materials and Methods We evaluated the reproducibility of the Baveno classification using data from the Santiago Obstructive Sleep Apnea (SantOSA) study. The groups were labeled Baveno A (minor symptoms and comorbidities), B (severe symptoms and minor comorbidities), C (minor symptoms and severe comorbidities), and D (severe symptoms and comorbidities). Within-group comparisons were performed using analysis of variance (ANOVA) and post hoc tests. The associations between groups and incident cardiovascular mortality were determined through the Mantel-Cox and Cox proportional hazard ratios (HRs) adjusted by covariables. Results A total of 1,300 OSA patients were included (Baveno A: 27.7%; B: 28%; C: 16.8%; and D: 27.5%). The follow-up was of 5.4 years. Compared to Baveno A, the fullyadjusted risk of cardiovascular mortality with Baveno B presented an HR of 1.38 (95% confidence interval [95%CI]: 0.14–13.5; p¼0.78); with Baveno C, it was of 1.71 (95%CI: 0.18–16.2; p¼0.63); and, with Baveno D, of 1.04 (95%CI: 0.12–9.2; p¼0.98). We found no interactions involving Baveno group, sex and OSA severity. Discussion Among OSA patients, the Baveno classification can describe different subgroups. However, its utility in identifying incident cardiovascular mortality is unclear. Long-term follow-up studies and the inclusion of demographic variables in the classification could improve its ability to detect a high-risk phenotype associated with cardiovascular mortality. Conclusion The Baveno classification serves as a valuable method for categorizing varying groups of patients afflicted with OSA. Nevertheless, its precision in identifying occurrence of cardiovascular mortality is still unclear.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.nefroe.2024.01.016
https://hdl.handle.net/10459.1/465679
url https://doi.org/10.1016/j.nefroe.2024.01.016
https://hdl.handle.net/10459.1/465679
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.nefroe.2024.01.016
Nefrologia, 2023, vol 43, p. 77-84
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Sociedad Española de Nefrología, 2023
Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv cc-by-nc-nd (c) Sociedad Española de Nefrología, 2023
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415797500149760
spelling Isolation and characterization of exosome-enriched urinary extracellular vesicles from Dent's disease type 1 Spanish patientsAislamiento y caracterización de vesículas extracelulares enriquecidas en exosomas en pacientes españoles con enfermedad de Dent 1Burballa , CarlaDuran, MònicaMartínez, CristinaAriceta ,GemaCantero Recasens, GerardMeseguer , AnnaObstructive sleep apneaCardiovascularHypoxiaLatinoPrecision medicineEnfermedad de DentExosomasVesículas extracelularesDD1CLCN5Objective To evaluate the clinical utility of the Baveno classification in predicting incident cardiovascular mortality after five years of follow-up in a clinic-based cohort of patients with obstructive sleep apnea (OSA). Materials and Methods We evaluated the reproducibility of the Baveno classification using data from the Santiago Obstructive Sleep Apnea (SantOSA) study. The groups were labeled Baveno A (minor symptoms and comorbidities), B (severe symptoms and minor comorbidities), C (minor symptoms and severe comorbidities), and D (severe symptoms and comorbidities). Within-group comparisons were performed using analysis of variance (ANOVA) and post hoc tests. The associations between groups and incident cardiovascular mortality were determined through the Mantel-Cox and Cox proportional hazard ratios (HRs) adjusted by covariables. Results A total of 1,300 OSA patients were included (Baveno A: 27.7%; B: 28%; C: 16.8%; and D: 27.5%). The follow-up was of 5.4 years. Compared to Baveno A, the fullyadjusted risk of cardiovascular mortality with Baveno B presented an HR of 1.38 (95% confidence interval [95%CI]: 0.14–13.5; p¼0.78); with Baveno C, it was of 1.71 (95%CI: 0.18–16.2; p¼0.63); and, with Baveno D, of 1.04 (95%CI: 0.12–9.2; p¼0.98). We found no interactions involving Baveno group, sex and OSA severity. Discussion Among OSA patients, the Baveno classification can describe different subgroups. However, its utility in identifying incident cardiovascular mortality is unclear. Long-term follow-up studies and the inclusion of demographic variables in the classification could improve its ability to detect a high-risk phenotype associated with cardiovascular mortality. Conclusion The Baveno classification serves as a valuable method for categorizing varying groups of patients afflicted with OSA. Nevertheless, its precision in identifying occurrence of cardiovascular mortality is still unclear.Antecedentes y objetivo La enfermedad de Dent tipo 1 (DD1) es una patología hereditaria rara ligada al cromosoma X causado por mutaciones en el CLCN5 que se caracteriza principalmente por una disfunción del túbulo proximal, hipercalciuria, nefrolitiasis/nefrocalcinosis, enfermedad renal crónica progresiva y proteinuria de bajo peso molecular, rasgo distintivo de la enfermedad. Actualmente, no existe un tratamiento curativo específico, únicamente sintomático y no previene la progresión de la enfermedad. En este estudio hemos aislado y caracterizado las vesículas extracelulares urinarias (uEVs) enriquecidas en exosomas que nos permitirán identificar biomarcadores asociados a la progresión de DD1 y una mejor comprensión de las bases fisiopatológicas de la misma. Materiales y métodos : A través de una convocatoria nacional de la Sociedad Española de Nefrología (SEN) y la Sociedad Española de Nefrología Pediátrica (AENP), se obtuvieron orinas de pacientes y controles de distintos hospitales españoles, las cuales se procesaron para obtener los uEVS. Los datos de estos pacientes fueron proporcionados por los respectivos nefrólogos y/o extraídos del registro RENALTUBE. Los uEVs se aislaron mediante ultracentrifugación, fueron caracterizados morfológicamente y se extrajo su contenido de proteína y microRNA. Resultados: Se reclutaron 25 pacientes y 10 controles, de los cuales se procesaron las orinas para aislar los uEVs. Nuestros resultados mostraron que la concentración relativa de uEVs/mL es menor en pacientes comparado con controles (0,26 × 106 uEVs/mL vs 1,19 × 106 uEVs/mL, p < 0.01). Además, se vio que los uEVs de los pacientes eran significativamente más grandes que los de los sujetos control (diámetro medio: 187,8 nm vs 143,6 nm, p < 0.01). Finalmente, nuestros datos demostraron que se había extraído correctamente RNA tanto de los exosomas de pacientes como de controles. Conclusiones: En este trabajo describimos el aislamiento y caracterización de uEVs de pacientes de la enfermedad de Dent 1 y controles sanos, útiles para el posterior estudio de moléculas cargo diferencialmente expresadas en esta patología.Elsevier2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1016/j.nefroe.2024.01.016https://hdl.handle.net/10459.1/465679reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.1016/j.nefroe.2024.01.016Nefrologia, 2023, vol 43, p. 77-84cc-by-nc-nd (c) Sociedad Española de Nefrología, 2023Attribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:repositori.udl.cat:10459.1/4656792026-06-24T12:42:17Z
score 15.812429

Similares en LA Referencia