Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo

To interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation in three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration and focusing as provided by infrared light and mu...

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Detalles Bibliográficos
Autores: Sortino, Rosalba, Cunquero, Marina, Castro Olvera, Gustavo, Gelabert, Ricardo, Moreno, Miquel, Riefolo, Fabio, Matera, Carlo, Fernàndez Castillo, Noèlia, Agnetta, Luca, Decker, Michael, Lluch, José M., Hernando, Jordi, Loza Álvarez, Pablo, Gorostiza Langa, Pablo Ignacio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/203764
Acceso en línea:https://hdl.handle.net/2445/203764
Access Level:acceso abierto
Palabra clave:Fotofarmacologia
Optoelectrònica
Circuit neuronal
Photopharmacology
Neural circuitry
Optoelectronics
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spelling Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in VivoSortino, RosalbaCunquero, MarinaCastro Olvera, GustavoGelabert, RicardoMoreno, MiquelRiefolo, FabioMatera, CarloFernàndez Castillo, NoèliaAgnetta, LucaDecker, MichaelLluch, José M.Hernando, JordiLoza Álvarez, PabloGorostiza Langa, Pablo IgnacioFotofarmacologiaOptoelectrònicaCircuit neuronalPhotopharmacologyNeural circuitryOptoelectronicsTo interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation in three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration and focusing as provided by infrared light and multiphoton excitation, and has promoted two-photon photopharmacology and optogenetics. However, three-photon brain stimulation in vivo remains to be demonstrated. We report the regulation of neuronal activity in zebrafish larvae by three-photon excitation of a photoswitchable muscarinic agonist at 50 pM, a billion-fold lower concentration than used for uncaging, and with mid-infrared light of 1560 nm, the longest reported photoswitch wavelength. Robust, physiologically relevant photoresponses allow modulating brain activity in wild-type animals with spatiotemporal and pharmacological precision. Computational calculations predict that azobenzene-based ligands have high three-photon absorption cross-section and can be used directly with pulsed infrared light. The expansion of three-photon pharmacology will deeply impact basic neurobiology and neuromodulation phototherapiesWiley-VCH2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/203764Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1002/anie.202311181Angewandte Chemie, 2023, vol. 62, num. 51https://doi.org/10.1002/anie.202311181cc by-nc (c) Sortino, Rosalba et al, 2023http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2037642026-05-27T06:46:51Z
dc.title.none.fl_str_mv Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
title Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
spellingShingle Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
Sortino, Rosalba
Fotofarmacologia
Optoelectrònica
Circuit neuronal
Photopharmacology
Neural circuitry
Optoelectronics
title_short Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
title_full Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
title_fullStr Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
title_full_unstemmed Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
title_sort Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo
dc.creator.none.fl_str_mv Sortino, Rosalba
Cunquero, Marina
Castro Olvera, Gustavo
Gelabert, Ricardo
Moreno, Miquel
Riefolo, Fabio
Matera, Carlo
Fernàndez Castillo, Noèlia
Agnetta, Luca
Decker, Michael
Lluch, José M.
Hernando, Jordi
Loza Álvarez, Pablo
Gorostiza Langa, Pablo Ignacio
author Sortino, Rosalba
author_facet Sortino, Rosalba
Cunquero, Marina
Castro Olvera, Gustavo
Gelabert, Ricardo
Moreno, Miquel
Riefolo, Fabio
Matera, Carlo
Fernàndez Castillo, Noèlia
Agnetta, Luca
Decker, Michael
Lluch, José M.
Hernando, Jordi
Loza Álvarez, Pablo
Gorostiza Langa, Pablo Ignacio
author_role author
author2 Cunquero, Marina
Castro Olvera, Gustavo
Gelabert, Ricardo
Moreno, Miquel
Riefolo, Fabio
Matera, Carlo
Fernàndez Castillo, Noèlia
Agnetta, Luca
Decker, Michael
Lluch, José M.
Hernando, Jordi
Loza Álvarez, Pablo
Gorostiza Langa, Pablo Ignacio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fotofarmacologia
Optoelectrònica
Circuit neuronal
Photopharmacology
Neural circuitry
Optoelectronics
topic Fotofarmacologia
Optoelectrònica
Circuit neuronal
Photopharmacology
Neural circuitry
Optoelectronics
description To interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation in three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration and focusing as provided by infrared light and multiphoton excitation, and has promoted two-photon photopharmacology and optogenetics. However, three-photon brain stimulation in vivo remains to be demonstrated. We report the regulation of neuronal activity in zebrafish larvae by three-photon excitation of a photoswitchable muscarinic agonist at 50 pM, a billion-fold lower concentration than used for uncaging, and with mid-infrared light of 1560 nm, the longest reported photoswitch wavelength. Robust, physiologically relevant photoresponses allow modulating brain activity in wild-type animals with spatiotemporal and pharmacological precision. Computational calculations predict that azobenzene-based ligands have high three-photon absorption cross-section and can be used directly with pulsed infrared light. The expansion of three-photon pharmacology will deeply impact basic neurobiology and neuromodulation phototherapies
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/203764
url https://hdl.handle.net/2445/203764
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/anie.202311181
Angewandte Chemie, 2023, vol. 62, num. 51
https://doi.org/10.1002/anie.202311181
dc.rights.none.fl_str_mv cc by-nc (c) Sortino, Rosalba et al, 2023
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc (c) Sortino, Rosalba et al, 2023
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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