Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco

BACKGROUND: Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites' transmission cycles. The present study investigates the role of rodent species as potential reservoirs o...

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Autores: Echchakery, Mohamed, Chicharro, Carmen, Boussaa, Samia, Nieto Martinez, Francisco Javier, Carrillo, Eugenia, Ortega, Sheila, Moreno, Javier, Boumezzough, Ali
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/6832
Acceso en línea:http://hdl.handle.net/20.500.12105/6832
Access Level:acceso abierto
Palabra clave:Animals
DNA, Protozoan
Disease Reservoirs
Endemic Diseases
Female
Gerbillinae
Humans
Leishmania infantum
Leishmania tropica
Leishmaniasis, Cutaneous
Leishmaniasis, Visceral
Male
Mice
Morocco
Rats
Rodentia
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spelling Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in MoroccoEchchakery, MohamedChicharro, CarmenBoussaa, SamiaNieto Martinez, Francisco JavierCarrillo, EugeniaOrtega, SheilaMoreno, JavierBoumezzough, AliAnimalsDNA, ProtozoanDisease ReservoirsEndemic DiseasesFemaleGerbillinaeHumansLeishmania infantumLeishmania tropicaLeishmaniasis, CutaneousLeishmaniasis, VisceralMaleMiceMoroccoRatsRodentiaBACKGROUND: Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites' transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. METHODS: Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. RESULTS: A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11 M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). CONCLUSIONS: To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco.BioMed Central (BMC)20182018-12-1220172017-10-0220172017-10-02journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/6832reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2CC0 1.0 Universalhttps://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/68322026-06-12T12:43:37Z
dc.title.none.fl_str_mv Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
title Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
spellingShingle Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
Echchakery, Mohamed
Animals
DNA, Protozoan
Disease Reservoirs
Endemic Diseases
Female
Gerbillinae
Humans
Leishmania infantum
Leishmania tropica
Leishmaniasis, Cutaneous
Leishmaniasis, Visceral
Male
Mice
Morocco
Rats
Rodentia
title_short Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
title_full Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
title_fullStr Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
title_full_unstemmed Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
title_sort Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
dc.creator.none.fl_str_mv Echchakery, Mohamed
Chicharro, Carmen
Boussaa, Samia
Nieto Martinez, Francisco Javier
Carrillo, Eugenia
Ortega, Sheila
Moreno, Javier
Boumezzough, Ali
author Echchakery, Mohamed
author_facet Echchakery, Mohamed
Chicharro, Carmen
Boussaa, Samia
Nieto Martinez, Francisco Javier
Carrillo, Eugenia
Ortega, Sheila
Moreno, Javier
Boumezzough, Ali
author_role author
author2 Chicharro, Carmen
Boussaa, Samia
Nieto Martinez, Francisco Javier
Carrillo, Eugenia
Ortega, Sheila
Moreno, Javier
Boumezzough, Ali
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Animals
DNA, Protozoan
Disease Reservoirs
Endemic Diseases
Female
Gerbillinae
Humans
Leishmania infantum
Leishmania tropica
Leishmaniasis, Cutaneous
Leishmaniasis, Visceral
Male
Mice
Morocco
Rats
Rodentia
topic Animals
DNA, Protozoan
Disease Reservoirs
Endemic Diseases
Female
Gerbillinae
Humans
Leishmania infantum
Leishmania tropica
Leishmaniasis, Cutaneous
Leishmaniasis, Visceral
Male
Mice
Morocco
Rats
Rodentia
description BACKGROUND: Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites' transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. METHODS: Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. RESULTS: A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11 M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). CONCLUSIONS: To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-10-02
2017
2017-10-02
2018
2018-12-12
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/6832
url http://hdl.handle.net/20.500.12105/6832
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC0 1.0 Universal
https://creativecommons.org/publicdomain/zero/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC0 1.0 Universal
https://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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